24 documents found, page 1 of 3
Comparative RNA-Seq analysis of S. epidermidis underSEP1 phage infection during...
Silva, Maria Daniela; Pinto, Graça; França, Angela; Azeredo, Joana; Melo, Luís Daniel RodriguesBacteria in nature often exist in the stationary phase, where antimicrobials, including phages, are less effective. The SEP1 phage was shown to reduce stationary S. epidermidis cells. This study analysed the response of both exponential and stationary cells to SEP1 infection. For that, RNA was extracted at 0, 5, 15, and 30 min post-infection and analysed by RNA-seq. In exponential cells, only 3 genes were upreg...
Phage-host interaction analysis using flow cytometry
Silva, Maria Daniela; Melo, Luís Daniel RodriguesBiofilm formation, a strategy of bacterial survival, is a significant concern in different areas, including health, where infectious biofilms are very difficult to combat with conventional antimicrobial therapies. Bacter-iophages, the viruses that infect bacteria, are promising agents to prevent and control biofilm-related infections. This chapter describes a series of standard procedures that can be used to st...
Phage SEP1 hijacks Staphylococcus epidermidis stationary cells metabolism to re...
Silva, Maria Daniela; Pinto, Graça; França, Ângela Maria Oliveira Sousa; Azeredo, Joana; Melo, Luís Daniel RodriguesIn nature, bacteria often survive in a stationary state with low metabolic activity. Phages use the metabolic machinery of the host cell to replicate, and, therefore, their efficacy against non-dividing cells is usually limited. Nevertheless, it was previously shown that the Staphylococcus epidermidis phage SEP1 has the remarkable capacity to actively replicate in stationary-phase cells, reducing their numbers....
Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella...
Vinayakumar, K. B.; Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna M.Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ...
Liposomes loaded with the pneumococcal endolysin MSlys: From in vitro character...
Silva, Maria Daniela; Paris, Juan Luis; Ray, Kaelin; Gama, F. M.; Silva, Bruno F. B.; Remenshneider, A. K.; Sillankorva, SannaIntroduction: The increasing antibiotic resistance triggered interest in novel antimicrobials as well as delivery systems that allow a topical and targeted delivery. However, biological barriers, such as the skin or the tympanic membrane (TM), may hinder the success of the therapy. Drug permeation has been extensively studied in the context of transdermal delivery, but only recently started to be explored for t...
Exploring endolysin-loaded liposomes for a transtympanic treatment of S. pneumo...
Silva, Maria Daniela; Paris, Juan Luis; Ray, Kaelin; Gama, F. M.; Silva, Bruno F. B.; Remenshneider, A. K.; Sillankorva, SannaOtitis media, the main reason for which antibiotics are prescribed in childhood, is often caused by Streptococcus pneumoniae. The exogenous use of recombinantly produced endolysins, peptidoglycan hydrolases encoded by bacteriophages at the end of their lytic cycle, have been shown to be very effective against this pathogen. To increase bioavailability, and consequently reduce the probability of a recurrent or c...
Ex vivo transtympanic permeation of the liposome encapsulated S. pneumoniae end...
Silva, Maria Daniela; Ray, Kaelin; Gama, F. M.; Remenschneider, Aaron K.; Sillankorva, SannaAn increase in bacterial resistance to systemic antibiotics has sparked interest into alternative antimicrobial compounds as well as methods for effective local, non-invasive drug delivery. Topical treatments, however, may be hindered by the presence of biological barriers, such as the tympanic membrane in the case of otitis media. Herein, the transtympanic permeation ability of liposomes loaded with the pneumo...
Targeted killing of ocular Streptococcus pneumoniae by the phage endolysin MSlys
Silva, Maria Daniela; André, Camille; Bispo, Paulo J. M.This study describes the translational potential of the pneumococcal endolysin MSlys as a novel approach to uniquely target and kill Streptococcus pneumoniae causing ocular infections.; This study was partially supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit. MDS was supported from a FCT doctoral fellowship, reference SF...
The clinical path to deliver encapsulated phages and lysins
Pinto, Ana; Silva, Maria Daniela; Pastrana, Lorenzo M; Bañobre-López, Manuel; Sillankorva, SannaThe global emergence of multidrug-resistant pathogens is shaping the current dogma regarding the use of antibiotherapy. Many bacteria have evolved to become resistant to conventional antibiotherapy, representing a health and economic burden for those afflicted. The search for alternative and complementary therapeutic approaches has intensified and revived phage therapy. In recent decades, the exogenous use of l...
Author Correction: Escherichia coli and Salmonella Enteritidis dual-species bio...
Milho, C.; Silva, Maria Daniela; Alves, Diana Filipa Barros; Oliveira, Hugo Alexandre Mendes; Sousa, C.; Pastrana, Lorenzo M.; Azeredo, JoanaCorrection to: Scientifc Reports https://doi.org/10.1038/s41598-019-54847-y, published online 03 December 2019; (undefined); info:eu-repo/semantics/publishedVersion