11 documents found, page 1 of 2
Seasonal proteome variation in intertidal shrimps under a natural setting: conn...
Madeira, D.; Araújo, J. E.; Madeira, C.; Mendonça, V.; Vitorino, R.; Vinagre, C.; Diniz, M. S.The ability of intertidal organisms to maintain their performance via molecular and physiological adjustments under low tide, seasonal fluctuations and extreme events ultimately determines population viability. Analyzing this capacity in the wild is extremely relevant since intertidal communities are under increased climate variability owing to global changes. We addressed the seasonal proteome signatures of a ...
Tetracycline and rifampicin induced a viable but nonculturable state in Staphyl...
Carvalhais, Virgínia Maria Dinis; Perez-Cabezas, B.; Oliveira, Cátia; Vitorino, R.; Vilanova, Manuel; Cerca, NunoAIM: The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates. MATERIALS & METHODS: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumerati...
Mudas de cana-de-açúcar influenciadas pelo manejo com herbicidas
Silva, T. P.; Azania, C. A.M.; Xavier, M. A.; Perecin, D. [UNESP]; Vitorino, R.Made available in DSpace on 2018-12-11T16:52:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2019-10-09T18:29:38Z : No. of bitstreams: 1 S0100-83582018000100202.pdf: 766792 bytes, checksum: fd579ebce5cd9b5ea790021ff6b6e09d (MD5); This research aimed at studying herbicides selectivity on individuals from three sugarcane families after different chemical managements in pri...
Negative synergistic impacts of ocean warming and acidification on the survival...
Araújo, J.E.; Madeira, D.; Vitorino, R.; Repolho, T.; Rosa, R.; Diniz, M.Global change is impacting aquatic ecosystems, with high risks for food production. However, the molecular underpinnings of organismal tolerance to both ocean warming and acidification are largely unknown. Here we tested the effect of warming and acidification in a 42-day experiment on a commercial temperate fish, the gilt-head seabream Sparus aurata. Juvenile fish were exposed to control (C 18 °C pH 8), ocean ...
Tutorials in Bioinformatics for Biological Science [editorial]
Vitorino, R.; Carvalho, P.; Penque, DeborahOne of the major challenges of the past few years is the integration of Omics data to disclose its biological meaning. With the advances in Omics technologies the complexity and amount of retrieved data has increased, making difficult and time-consuming its integrative analysis. Even so, the integration of a wide spectrum of Omics data sets is essential to promote translational research. Bioinformatics tools al...
Ocean warming alters cellular metabolism and induces mortality in fish early li...
Madeira, Diana; Araújo, J.E.; Vitorino, R.; Capelo, J.L.; Vinagre, Catarina; Diniz, Mário S.Climate change has pervasive effects on marine ecosystems, altering biodiversity patterns, abundance and distribution of species, biological interactions, phenology, and organisms' physiology, performance and fitness. Fish early life stages have narrow thermal windows and are thus more vulnerable to further changes in water temperature. The aim of this study was to address the sensitivity and underlying molecul...
Dormancy within Staphylococcus epidermidis biofilms: an immunoproteomic charact...
Carvalhais, Virgínia Maria Dinis; Cerca, Nuno; Vilanova, M.; Vitorino, R.Dormant bacteria within biofilms contribute to biofilm heterogeneity. Consequently, physiological heterogeneity of biofilms may influence host immune response and tolerance to antibiotics. Recently, we described an in vitro model to modulate dormancy in S. epidermidis biofilms. Here, we present a study based on immunoproteomics, where we compared the reactive profile of S. epidermidis biofilm proteins with prev...
Mitochondria proteome profiling: a comparative analysis between gel- and gel-fr...
Ferreira, R.; Rocha, H.; Almeida, V.; Padrão, A.L.; Santa, C.; Vilarinho, L.; Amado, F.; Vitorino, R.Mitochondrial proteomics emerged aiming to disclose the dynamics of mitochondria under various pathophysiological conditions. In the present study we investigated the relative merits of gel-based (2DE and SDS-LC) and gel-free (2D-LC) protein separation approaches and protein identification algorithms (Mascot and Paragon) in the proteome profiling of mitochondria isolated from cultured fibroblasts, a sample trad...
Characterization of mitochondrial proteome in a severe case of ETF-QO deficiency
Rocha, H.; Ferreira, R.; Carvalho, J.; Vitorino, R.; Santa, C.; Lopes, L.; Gregersen, N.; Vilarinho, L.; Amado, F.Multiple acyl-CoA dehydrogenase deficiency (MADD) is a mitochondrial fatty acid oxidation disorder caused by mutations that affect electron transfer flavoprotein (ETF) or ETF:ubiquinone oxidoreductase (ETF-QO) or even due to unidentified disturbances of riboflavin metabolism. Besides all the available data on the molecular basis of FAO disorders, including MADD, the pathophysiological mechanisms underlying clin...
Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. ...
Silva, J.; Carvalho, A. S.; Ferreira, R.; Vitorino, R.; Amado, F.; Domingues, P.; Teixeira, P.; Gibbs, P. A.Aims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spraydrying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20ºC and heat treated at 57ºC. Cells grown under noncontrolled pH were more resistant to both drying and he...