10 documents found, page 1 of 1

Near-Infrared Light Activated Formulation for the Spatially Controlled Release ...

The CRISPR/Cas9 system has emerged as a promising platform for gene editing; however, the lack of an efficient and safe delivery system to introduce it into cells continues to hinder clinical translation. Here, we report a rationally designed gene-editing nanoparticle (NP) formulation for brain applications: an sgRNA:Cas9 ribonucleoprotein complex is immobilized on the NP surface by oligonucleotides that are co...

A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts

Messenger RNA (mRNA)-based therapies offer enhanced control over the production of therapeutic proteins for many diseases. Their clinical implementation warrants formulations capable of delivering them safely and effectively to target sites. Owing to their chemical versatility, polymeric nanoparticles can be designed by combinatorial synthesis of different ionizable, cationic, and aromatic moieties to modulate ...

Author Correction: Efficient spatially targeted gene editing using a near-infra...

Efficient spatially targeted gene editing using a near-infrared activatable pro...

Spatial control of gene expression is critical to modulate cellular functions and deconstruct the function of individual genes in biological processes. Light-responsive gene-editing formulations have been recently developed; however, they have shown limited applicability in vivo due to poor tissue penetration, limited cellular transfection and the difficulty in evaluating the activity of the edited cells. Here,...

Therapeutic Approaches for Stroke: A Biomaterials Perspective

Stroke is a leading cause of death worldwide and poses significant societal and healthcare challenges due to functional impairment of the brain. In order to fully restore brain function, innovative approaches have aimed to regenerate the injured tissue and to restore neuronal circuitry. In the last 5 years, stem cells have been consistently explored in clinical trials for tissue regeneration. Recent technologic...

A light-triggerable formulation to control the stability of pro-angiogenic tran...

A high-throughput screening platform to identify nanocarriers for efficient del...

The quantification of health in the practice of Traditional Chinese Medicine (T...

Is it possible to quantify a “healthy” body in Traditional Chinese Medicine (TCM)? What is the conception of the body that underlies this ancient practice (Taoism and Confucianism)? Can we draw any parallels with our pre-Socratic influence? What relevance does this theme have in the subject of health care today? These are the questions we propose to think about, in order to contribute to a reflection on the sub...

A light-triggerable nanoparticle library for the controlled release of non-codi...

RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity a...

Senior digital exclusion: life stories, generations and generational culture

The digital divide based on age is one of the main forms of digital exclusion within societies. In an increasingly aging society which is also increasingly mediated by technology it poses serious challenges both for excluded individuals and for the societies they integrate. It is important to better understand this phenomenon whose study, however, presents important challenges. One of the main challenges of stu...