14 documents found, page 1 of 2
Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endome...
Amaral, Ana; Cebola, Nélio; Zóstek-Mioduchowska, Anna; Rebordão, Maria Rosa; Kordowitzki, Paweł; Skarzynski, Dariusz; Ferreira-Dias, GraçaMyeloperoxidase is an enzyme released by neutrophils when neutrophil extracellular traps (NETs) are formed. Besides myeloperoxidase activity against pathogens, it was also linked to many diseases, including inflammatory and fibrotic ones. Endometrosis is a fibrotic disease of the mare endometrium, with a large impact on their fertility, where myeloperoxidase was shown to induce fibrosis. Noscapine is an alkaloi...
Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endome...
Amaral, Ana; Cebola, Nélio; Szóstek-Mioduchowska, Anna; Rebordão, Maria Rosa; Kordowitzki, Paweł; Skarzynski, Dariusz; Ferreira-Dias, GraçaMyeloperoxidase is an enzyme released by neutrophils when neutrophil extracellular traps (NETs) are formed. Besides myeloperoxidase activity against pathogens, it was also linked to many diseases, including inflammatory and fibrotic ones. Endometrosis is a fibrotic disease of the mare endometrium, with a large impact on their fertility, where myeloperoxidase was shown to induce fibrosis. Noscapine is an alkaloi...
Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine Endome...
Amaral, Ana; Cebola, Nélio; Szóstek-Mioduchowska, Anna; Rebordão, Maria Rosa; Kordowitzki, Paweł; Skarzynski, Dariusz; Ferreira-Dias, GraçaMyeloperoxidase is an enzyme released by neutrophils when neutrophil extracellular traps (NETs) are formed. Besides myeloperoxidase activity against pathogens, it was also linked to many diseases, including inflammatory and fibrotic ones. Endometrosis is a fibrotic disease of the mare endometrium, with a large impact on their fertility, where myeloperoxidase was shown to induce fibrosis. Noscapine is an alkaloi...
Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibr...
Alpoim-Moreira, Joana; Fernandes, Carina; Pimenta, J.; Bliebernicht, Miguel; Rebordão, Maria Rosa; Castelo-Branco, Pedro; Szostek-Mioduchowska, AnnaEndometrium type I (COL1) and III (COL3) collagen accumulation, periglandular fibrosis and mare infertility characterize endometrosis. Metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) are involved in collagen turnover. Since epigenetic changes may control fibroproliferative diseases, we hypothesized that epigenetic mechanisms could modulate equine endometrosis. ...
Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibr...
Alpoim-Moreira, Joana; Fernandes, Carina; Pimenta, Jorge; Bliebernicht, Miguel; Rebordão, Maria Rosa; Castelo-Branco, Pedro; Szóstek-Mioduchowska, AnnaEndometrium type I (COL1) and III (COL3) collagen accumulation, periglandular fibrosis and mare infertility characterize endometrosis. Metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) are involved in collagen turnover. Since epigenetic changes may control fibroproliferative diseases, we hypothesized that epigenetic mechanisms could modulate equine endometrosis. ...
Collagen and microvascularization in placentas from young and older mares
Silva, Ana Catarina Neto; Costa, Ana Luísa; Teixeira, Ana; Alpoim-moreira, Joana; Fernandes, Carina; Fradinho, M.J.; Rebordão, Maria RosaIn older mares, increasing collagen fibers (fibrosis) in the endometrium and oviduct predisposes to sub-fertility and infertility. In this study, (i) gene transcription of collagen (qPCR: COL1A1, COL1A2, COL3A1, COL5A1); (ii) total collagen protein (hydroxyproline); (iii) collagen distribution (Picrosirius red staining; polarized light microscopy); and (iv) microvascular density (Periodic acid-Schiff staining),...
Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
Alpoim-Moreira, Joana; Fernandes, Carina; Rebordão, Maria Rosa; Costa, Ana Luísa; BliebernichT, Miguel; Nunes, Telmo; Szóstek-Mioduchowska, AnnaCollagen pathological deposition in equine endometrium (endometrosis) is responsible for infertility. Kenney and Doig’s endometrial biopsy histopathological classification is the gold standard method for endometrosis evaluation, whereby blood biomarkers identification would be less invasive and could provide additional information regarding endometrosis diagnosis and fertility prognosis. This study aimed to ide...
Enzymes present in neutrophil extracellular traps may stimulate the fibrogenic ...
Rebordão, Maria Rosa; Amaral, Ana; Fernandes, Carina; Silva, E; Lukasik, Karolina; Szóstek-Mioduchowska, Anna; Bravo, Pedro; Galvão, AntónioEndometrosis is a fibrotic disease in mare endometrium whose pathological mechanisms remain obscure. Prostaglandin (PG)F2α, despite modulating reproductive physiological processes, may also provoke local pathological collagen deposition (fibrogenesis). Neutrophil extracellular traps (NETs) released during inflammation have been linked to fibrogenesis in several tissues. We have previously shown that enzymes fou...
The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen...
Amaral, Ana; Fernandes, Carina; Szostek-Mioduchowska, Anna; Lukasik, Karolina; Rebordão, Maria Rosa; Pinto-Bravo, Pedro; Skarzynski, Dariusz JanABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and a...
Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen de...
Amaral, Ana; Fernandes, Carina; Szóstek-Mioduchowska, Anna; Rebordão, Maria Rosa; Skarzynski, Dariusz Jan; Ferreira-Dias, GraçaABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the...