4 documents found, page 1 of 1
A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transep...
Hevia, Arancha; Ruas-Madiedo, Patricia; Faria, Miguel Angelo; Petit, Valérie; Alves, Bruna; Alvito, Paula; Arranz, Elena; Bastiaan-Net, ShannaAssessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biolog...
Standardization of in vitro digestibility and DIAAS method based on the static ...
Egger, Lotti; Sousa, Raquel; Recio, Isidra; Abbühl, Lychou; Brügger, Cédric; Hernández, Marta Santos; Macierzanka, Adam; Szumala, PatrycjaBackground: The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, ...
INFOGEST static in vitro simulation of gastrointestinal food digestion
Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; Assunção, Ricardo; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, ClaireDeveloping a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on a...
The harmonized INFOGEST in vitro digestion method: From knowledge to action
Egger, Lotti; Menard, Olivia; Delgado-Andrade, Cristina; Alvito, Paula; Assunção, Ricardo; Balance, Simon; Barberá, Reyes; Brodkorb, AndreWithin the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network...