Este projecto compreende 3 actividades principais: a) Avaliação do status quo do pomar e a incidência de estenfiliose. A avaliação das práticas culturais efetuadas pelos diferentes produtores nos últimos 5 anos, com recurso a inquéritos, a avaliação da flora existente nos pomares e o acompanhamento dos pomares durante o período de execução do projeto contribuirá para se estabelecerem possíveis relações entre as...
Grapevine is regarded as a highly profitable culture, being well spread worldwide and mostly directed to the wine-producing industry. Practices to maintain the vineyard in healthy conditions are tenuous and are exacerbated due to abiotic and biotic stresses, where fungal grapevine trunk diseases (GTDs) play a major role. The abolishment of chemical treatments and the intensification of several management practi...
The aroma of grapes is cultivar dependent and is influenced by terroir, vineyard practices, and abiotic and biotic stresses. Trincadeira is a non-aromatic variety associated with low phenolic content and high sugar and organic acid levels. This cultivar, widely used in Portuguese wines, presents high susceptibility to Botrytis cinerea. This work aimed to characterise the volatile profile of Trincadeira grapes a...
The biotrophic fungus Erysiphe necator causes powdery mildew (PM) in grapevine. Phytohormones are major modulators of defensive responses in plants but the analysis of the hormonome associated with grapevine tolerance and susceptibility against this pathogen has not been elucidated. In this study, changes in hormonal profiling were compared between a tolerant (Vitis rupestris × riparia cv. 101-14 Millardet et d...
Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including one...
Botryosphaeria parva, Eutypa lata and Phomopsis viticola are ascomyceteous fungi responsible for severe canker and dieback in numerous woody plants. In grapevine, these pathogens colonise the wood mainly through pruning wounds, and the diseases gradually develop, leading to partial or total vine death. In the present study, the three fungal species were grown in Czapek Dox modifi ed medium. Under these conditio...
Previous surveys conducted in commercial nurseries found that different wood fungi, namely Cylindrocarpon spp., Botryosphaeriaceae, Phomopsis viticola and Phaeomoniella chlamydospora infect grapevine cuttings. Two fi eld trials were carried out to evaluate the effectiveness of cyprodinil + fl udioxonil, pyraclostrobin + metiram, fl udioxonil and cyprodinil to prevent or reduce natural infections caused by such ...
The effect of Phaeomoniella chlamydospora infection was evaluated in three grapevine cultivars, in relation to the variation in phenolic compounds within the host. Young plants of Vitis vinifera (cv. Chardonnay, Touriga Nacional and two clones of cv. Aragonez) were infected using two strains of Pa. chlamydospora and were harvested after fi ve months from inoculation. Overall, a localised increment in the amount...
Black foot disease is a serious disease of grapevine crops in most areas where vines are grown. Mainly two species of Cylindrocarpon, C. destructans and C. macrodidymum, are associated with this disease. Recent studies have revealed a tremendous molecular variation within the former but only slight molecular variation within the latter, indicating that C. destructans presents a complex of several species The pr...
Black-foot is an important grapevine disease, caused by the fungus Cylindrocarpon destructans, which causes a darkening of the basal-end of the plant, leading to early decline and death of young grapevines. Both, C. destructans and C. obtusisporum are considered responsible for this disease, although only the former was isolated in Portugal. Neonectria radicicola (teleomorph of C. destructans) is an Ascomycota ...