17 documents found, page 1 of 2
SuperSAGE digital expression analysis of differential growth rate in a European...
Louro, Bruno; Martins, Rute S.T.; Pinto, Patricia IS; Reinhardt, Richard; de Koning, Dirk-Jan; Canario, Adelino; Power, DeborahOne of the goals of the aquaculture industry is to understand and control growth associated traits through selective breeding. In the present study the molecular basis of growth heterogeneity in the European sea bass (Dicentrarchus labrax) was addressed. To establish growth heterogeneity in a group of hatchery bred sea bass individuals were tagged and their specific growth rates (SGR) determined at monthly inte...
Characterization and refinement of growth related quantitative trait loci in Eu...
Louro, Bruno; Kuhl, Heiner; Tine, Mbaye; de Koning, Dirk-Jan; Batargias, Costas; Volckaert, Filip A. M.; Reinhardt, Richard; Canario, AdelinoThe identification of genetic markers for traits of interest for aquaculture, such as growth, is an important step for the establishment of breeding programmes. As more genomic information becomes available the possibility of applying comparative genomics to identify and refine quantitative trait locus (QTLs) and potentially identify candidate genes responsible for the QTL effect may accelerate genetic improvem...
European sea bass genome and its variation provide insights into adaptation to ...
Tine, Mbaye; Kuhl, Heiner; Gagnaire, Pierre-Alexandre; Louro, Bruno; Desmarais, Erick; Martins, Rute S. T.; Hecht, Jochen; Knaust, FlorianThe European sea bass (Dicentrarchus labrax) is a temperate-zone euryhaline teleost of prime importance for aquaculture and fisheries. This species is subdivided into two naturally hybridizing lineages, one inhabiting the north-eastern Atlantic Ocean and the other the Mediterranean and Black seas. Here we provide a high-quality chromosome-scale assembly of its genome that shows a high degree of synteny with the...
PTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone pr...
Anjos, Liliana; Redruello, Begoña; Reinhardt, Richard; Canario, Adelino V. M.; Power, DeborahEndocrine factors play an essential role in the formation and turnover of the skeleton in vertebrates. In the present study sea bream vertebral bone transcripts for PTH1R and PTH3R were identified and the action of intermittent administration of parathyroid hormone related protein (PTHrP) on the proteome of vertebral bone was analysed. Treatment of immature sea bream (Sparus auratus, n = 6) for 5 days with homo...
mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditape...
Leite, Ricardo; Milan, Massimo; Coppe, Alessandro; Bortoluzzi, Stefania; Dos Anjos, António; Reinhardt, Richard; Saavedra, Carlos; Patarnello, T.Background: The Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have desi...
Comparative analysis of a teleost skeleton transcriptome provides insight into ...
Vieira, Florbela A.; Thorne, Michael A. S.; Stueber, K.; Darias, M.; Reinhardt, Richard; Clark, M. S.; Gisbert, Enric; Power, DeborahAn articulated endoskeleton that is calcified is a unifying innovation of the vertebrates, however the molecular basis of the structural divergence between terrestrial and aquatic vertebrates, such as teleost fish, has not been determined. In the present study long-read next generation sequencing (NGS, Roche 454 platform) was used to characterize acellular perichondral bone (vertebrae) and chondroid bone (gill ...
Transcriptome sequencing and microarray development for the Manila clam, Rudita...
Milan, Massimo; Coppe, Alessandro; Reinhardt, Richard; Cancela, Leonor; Leite, Ricardo; Saavedra, Carlos; Ciofi, Claudio; Chelazzi, GuidoAbstract Background The Manila clam, Ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. However, genomic resources for R. philippinarum are still extremely limited. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on aq...
Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) f...
Massa, S. I.; Pearson, G. A.; Aires, Tânia; Kube, M.; Olsen, J. L.; Reinhardt, Richard; Serrão, Ester; ARNAUD-HAOND, SophiePredicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyze...
Development of an oligo DNA microarray for the European sea bass and its applic...
Ferraresso, Serena; Milan, Massimo; Pellizzari, Caterina; Vitulo, Nicola; Reinhardt, Richard; Canario, Adelino V. M.; Patarnello, T.; Bargelloni, LucaAbstract Background The European sea bass (Dicentrarchus labrax) is a marine fish of great importance for fisheries and aquaculture. Functional genomics offers the possibility to discover the molecular mechanisms underlying productive traits in farmed fish, and a step towards the application of marker assisted selection methods in this species. To this end, we report here on the development of an oligo DNA micr...
An expressed sequence tag analysis of the intertidal brown seaweeds Fucus serra...
Pearson, G. A.; Hoarau, G.; Lago-Lestón, Asunción; Coyer, J. A.; Kube, M.; Reinhardt, Richard; Henckel, K.; Serrão, Ester; Corre, E.; Olsen, J. L.In order to aid gene discovery and uncover genes responding to abiotic stressors in stress-tolerant brown algae of the genus Fucus, expressed sequence tags (ESTs) were studied in two species, Fucus serratus and Fucus vesiculosus. Clustering of over 12,000 ESTs from three libraries for heat shock/recovery and desiccation/rehydration resulted in identification of 2,503, 1,290, and 2,409 unigenes from heat-shocked...