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Benchmarking commercially available value-added fractions with potential for pr...

Ferreira, Flávio; Reis, Alberto; Ortigueira, Joana; Lopes, Tiago

ABSTRACT: The urgent need to mitigate climate change requires finding sustainable and efficient alternatives to fossil fuel-based materials. Biosequestration by microalgae has been suggested as a potential method for climate change mitigation due to its environmentally friendly nature and ability to produce high-value compounds. However, the large-scale application of microalgal biorefineries faces significant ...

Data: 2025   |   Origem: Repositório do LNEG

Integrating social aspects in microalgal biorefineries: a Product Social Impact...

Ortigueira, Joana; Lopes, Tiago; Reis, Alberto; Gírio, Francisco

ABSTRACT: The ongoing climate change phenomenon requires the reduction of atmospheric CO2 concentrations. Microalgal biorefineries, which convert atmospheric CO2 into chemical energy, offer a viable alternative to fossil fuel-based industrial systems. This study assesses the social impacts of microalgal biorefineries using the Product Social Impact Life Cycle Assessment database approach, focusing on an industr...

Data: 2025   |   Origem: Repositório do LNEG

Fractionation of macroalgae carbohydrates using hydrothermal and dilute inorgan...

Martins, Pedro L.; Andrade, Cristiana; Duarte, Luís; Reis, Alberto; Pereira, Helena; Carvalheiro, Florbela

ABSTRACT: Furans are among the most important compounds derived from biomass, providing conversion pathways for sustainable alternatives to petroleum-based fuels and materials. Furfural, 5-hydroxymethylfurfural (5-HMF), and 5-methylfurfural (5-MF) are furans that can be obtained by carbohydrate dehydration under acidic conditions at elevated temperature and pressure. One of the mechanisms to produce these compo...

Data: 2025   |   Origem: Repositório do LNEG

Monitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytom...

Lopes da Silva, Teresa; Silva, Thiago; França, Bruna Thomazinho; Ribeiro, Belina; Reis, Alberto

ABSTRACT: Winery wastewater (WWW), if released untreated, poses a serious environmental threat due to its high organic load. In this study, Chlorella vulgaris was cultivated in diluted WWW to assess its suitability as a culture medium. Two outdoor cultivation systems-a 270 L raceway and a 40 L bubble column-were operated over 33 days using synthetic medium (control) and WWW. A flow cytometry (FC) protocol was i...

Data: 2025   |   Origem: Repositório do LNEG

Como o trabalho do LNEG apoia a concretização do PNEC: Plano Nacional de Energi...

Simoes, Sofia; Simões, Teresa; Barbosa, Juliana Pacheco; Rodrigues, Carlos; Azevedo, Pedro; Cardoso, João; Facão, Jorge; Costa, Paula Silva

Data: 2025   |   Origem: Repositório do LNEG

Biofuel from wastewater-grown microalgae: A biorefinery approach using hydrothe...

Silva, Thiago; de Aguiar do Couto, Eduardo; Assemany, Paula; Costa, Paula; Marques, Paula; Paradela, Filipe; Reis, Alberto; Calijuri, Maria Lucia

ABSTRACT: Third-generation biofuels from microalgae are becoming necessary for sustainable energy. In this context, this study explores the hydrothermal liquefaction (HTL) of microalgae biomass grown in wastewater, consisting of 30% Chlorella vulgaris, 69% Tetradesmus obliquus, and 1% cyanobacteria Limnothrix planctonica, and the subsequent upgrading of the produced bio-oil. The novelty of the work lies in inte...

Data: 2024   |   Origem: Repositório do LNEG

Enhancing microalgae biomass production: Exploring improved scraping frequency ...

Silva, Thiago; Pereira, Alexia Saleme Aona de Paula; Ferreira, Jessica; Lorentz, Juliana F.; de Assis, Marilia Luise; Assemany, Paula; Reis, Alberto

ABSTRACT: Recently, hybrid systems, such as those incorporating high-rate algal ponds (HRAPs) and biofilm reactors (BRs), have shown promise in treating domestic wastewater while cultivating microalgae. In this context, the objective of the present study was to determine an improved scraping frequency to maximize microalgae biomass productivity in a mix of industrial (fruit-based juice production) and domestic ...

Data: 2024   |   Origem: Repositório do LNEG

Municipal and industrial wastewater blending: Effect of the carbon/ nitrogen ra...

Pereira, Alexia Saleme Aona de Paula; Magalhães, Iara; Silva, Thiago; Reis, Alberto; de Aguiar do Couto, Eduardo; Calijuri, Maria Lucia

ABSTRACT: Municipal wastewater (MW) and industrial wastewater from juice processing (IWJ) were blended in different proportions to assess the effect of the carbon/nitrogen (C/N) ratio on pollutant removal, microalgal biomass (MB) cultivation, and the accumulation of carotenoids and biocompounds. MB development was not observed in treatments with higher C/N ratios (>30.67). The wastewater mixture favored the rem...

Data: 2024   |   Origem: Repositório do LNEG

Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production

Fontes, Afonso; Francisco, Ricardo; Ferreira, Frederico Castelo; Faria, Nuno Torres; Marques, Susana; Reis, Alberto; Moura, Patrícia; Lukasik, Rafal M.

ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further culti...

Data: 2024   |   Origem: Repositório do LNEG

Evaluation of different fractionation methods for the simultaneous protein and ...

Martins, Pedro L.; Duarte, Luís C.; Pereira, Helena; Reis, Alberto; Carvalheiro, Florbela

ABSTRACT: The production of high-value products from microalgae, one of the preferred emerging biorefineries' feedstocks, relies on the crucial step of biomass fractionation. In this work, the fractionation of Chlorella vulgaris and Scenedesmus obliquus biomass was tested for protein extraction using a wide range of physical, chemical, and enzymatic treatment combinations, including ultrasound, cell homogenizer...

Data: 2024   |   Origem: Repositório do LNEG

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