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Microwave hydrodiffusion and gravity: a green extraction technology for phenyle...

Sousa, Ana Sofia; Sousa, Sérgio; Silva, Inês V.; Reis, Celso A.; Coscueta, Ezequiel R.; Pintado, Maria Manuela

Watercress is a cruciferous vegetable and the primary natural source of phenylethyl isothiocyanate (PEITC), a powerful health-promoting agent. Conventional extraction of PEITC relies on harmful solvents, which limits sustainability. This study introduces a novel, solvent-free method—microwave hydrodiffusion and gravity (MHG)—to extract PEITC from watercress by-products (WCBP). Results showed that freezing WCBP ...



Effects of microencapsulated phenethyl isothiocyanate on gastrointestinal cance...

Abrantes, Rafaela; Ramos, Cátia C.; Coscueta, Ezequiel R.; Costa, João; Gomes, Joana; Gomes, Catarina; Reis, Celso A.; Pintado, Maria Manuela

Gastrointestinal cancers remain a global health burden, demanding more effective prevention and treatments. Phenethyl isothiocyanate (PEITC), a compound derived from cruciferous vegetables, stands out as a promising nutraceutical agent due to its chemopreventive and therapeutic properties. However, its therapeutic translation remains limited mainly due to its poor water solubility and rapid metabolism. Herein, ...


Microbots for colonic delivery of PEITC: using INFOGEST to optimize formulation...

Sousa, Ana Sofia; Coscueta, Ezequiel R.; Reis, Celso A.; Pintado, Manuela

Globally, gastrointestinal diseases impact 40% of the population and burden healthcare services. Inflammatory bowel disease (IBD) is a common disorder affecting over 6 million people worldwide, leading to significant morbidity and mortality. Therefore, novel approaches are needed to control colonic diseases effectively with minimal side effects.Using natural bioactive compounds such as isothiocyanates (ITCs) fr...


A solvent-free approach to extract phenylethyl isothiocyanate from nasturtium o...

Sousa, Ana Sofia; Coscueta, Ezequiel R.; Reis, Celso A.; Pintado, Manuela

This research aims to utilize MHG technology to produce extracts enriched with PEITC from watercress by-products and assess their antioxidant activity and total phenolic content.




Phenylethyl isothiocyanate: a bioactive agent for gastrointestinal health

Coscueta, Ezequiel R.; Sousa, Ana Sofia; Reis, Celso A.; Pintado, Maria Manuela

The incidence of gastrointestinal pathologies (cancer in particular) has increased progressively, with considerable morbidity and mortality, and a high economic impact on the healthcare system. The dietary intake of natural phytochemicals with certain bioactive properties has shown therapeutic and preventive effects on these pathologies. This includes the cruciferous vegetable derivative phenylethyl isothiocyan...


Whey protein-based hydrogels as phenylethyl isothiocyanate delivery systems

Sousa, Ana Sofia; Coscueta, Ezequiel R.; Reis, Celso A.; Pintado, Manuela

Phenylethyl isothiocyanate (PEITC), a glucosinolate hydrolysis product, is a bioactive compound present in some cruciferous vegetables, namely watercress, with interesting bioactivities such as anticancer, antioxidant and anti- inflammatory. Indeed, this phytochemical has been studied for its potential preventive and therapeutic effects on chronic diseases such as cancer1. Although PEITC is a promising health-p...



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