101 documents found, page 1 of 11
Impact of aging on the 6-OHDA-induced rat model of Parkinson’s disease
Barata-Antunes, Sandra; Teixeira, Fábio G.; Mendes-Pinheiro, Bárbara; Domingues, Ana V.; Vilaça-Faria, Helena; Marote, Ana; Silva, DeolindaParkinson’s disease (PD) is the second most common age-related neurodegenerative disorder. The neurodegeneration leading to incapacitating motor abnormalities mainly occurs in the nigrostriatal pathway due to the loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Several animal models have been developed not only to better understand the mechanisms underlying neurodegeneration but also t...
The Role of Biomaterials as Angiogenic Modulators of Spinal Cord Injury: Mimeti...
Rocha, Luís A.; Sousa, R. A.; Learmonth, David A.; Salgado, A. J.Spinal cord injury (SCI) represents an extremely debilitating condition for which no efficacious treatment is available. One of the main contributors to the inhospitable environment found in SCI is the vascular disruption that happens at the moment of injury that compromises the blood-spinal cord barrier (BSCB) and triggers a cascade of events that includes infiltration of inflammatory cells, ischemia and intra...
Posterior talar process as a suitable cell source for treatment of cartilage an...
Correia, Sandra I.; Silva-Correia, Joana; Pereira, H.; Canadas, Raphael Faustino; Morais, Alain; Frias, A. M.; Sousa, R. A.; van Dijk, C.N.Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues and investigate its potential as a new source of viable cells for application in tissue engineeri...
Clinical management of articular cartilage lesions
Vilela, C. M.; Correia, C.; Oliveira, Joaquim M.; Sousa, R. A.; Reis, R. L.; Espregueira-Mendes, JoãoArticular cartilage is extremely sensitive to traumatic lesions and natural repair is very limited. When regeneration occur the tissue found in the lesion site is mostly fibrocartilage with poor mechanical properties, rendering a poor long-term clinical outcome. Cartilage lesion is a common problem with an impressive clinical and economic impact. With a difficult diagnosis in an initial disease stage, the carti...
“Smart” hydrogels in tissue engineering and regenerative medicine applications
Bacelar, Ana Helena Dias; Cengiz, I. F.; Silva-Correia, Joana; Sousa, R. A.; Oliveira, J. M.; Reis, R. L.“Smart” hydrogels, also variously referred as “intelligent,” stimuliresponsive, stimuli-sensitive, or environmentally sensitive hydrogels, are three-dimensional hydrophilic network structures of polymer chains that are able to change their properties in response to environmental stimuli such as temperature, pH, light, magnetic and electric fields, and ionic strength or enzymatic environment. Systems with multip...
Job shop schedules analysis in the context of industry 4.0
Sousa, R. A.; Varela, M.L.R.; Alves, Cláudio; Machado, JoséIndustry 4.0 is announced as a fourth industrial revolution. The next level of evolution will comprehend the wide spread inclusion of machines sensors and big data analytics. Enterprise Resource Planning and Manufacturing Execution Systems will be the information management tools for the revolution. Different forms of optimization will be in the brink of development to answer this revolution needs. In this pape...
Influence of different surface modification treatments on silk biotextiles for ...
Ribeiro, V. P.; Almeida, L. R.; Martins, A. R.; Pashkuleva, I.; Marques, A. P.; Ribeiro, Ana S.; Silva, Carla J. S. M.; Bonifácio, Graça; Sousa, R. A.Biotextile structures from silk fibroin have demonstrated to be particularly interesting for tissue engineering (TE) applications due to their high mechanical strength, interconnectivity, porosity, and ability to degrade under physiological conditions. In this work, we described several surface treatments of knitted silk fibroin (SF) scaffolds, namely sodium hydroxide (NaOH) solution, ultraviolet radiation expo...
Tumor growth suppression induced by biomimetic silk fibroin hydrogels
Leping, Yan; Silva-Correia, Joana; Ribeiro, Viviana Pinto; Gonçalves, Vera Mónica Miranda; Correia, Cristina; Morais, Alain José Silva; Sousa, R. A.Protein-based hydrogels with distinct conformations which enable encapsulation or differentiation of cells are of great interest in 3D cancer research models. Conformational changes may cause macroscopic shifts in the hydrogels, allowing for its use as biosensors and drug carriers. In depth knowledge on how 3D conformational changes in proteins may affect cell fate and tumor formation is required. Thus, this st...
Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: in ...
Yan, Leping; Silva-Correia, Joana; Oliveira, Mariana B.; Vilela, Carlos; Pereira, H.; Sousa, R. A.; Mano, J. F.; Oliveira, A. L.; Oliveira, Joaquim M.Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (Silk-NanoCaP) layer for osteochondral defect (OCD) regeneration were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the Silk-NanoCaP layer. The scaffold presented compressive moduli of 0.4 MPa in wet state. Rabbit bone marrow ...
Hierarchical scaffolds enhance osteogenic differentiation of human Wharton’s je...
Canha-Gouveia, A.; Costa-Pinto, A. R.; Martins, Albino; Silva, N. A.; Faria, Susana; Sousa, R. A.; Salgado, A. J.; Sousa, Nuno; Reis, R. L.Hierarchical structures, constituted by polymeric nano and microfibers, have been considered promising scaffolds for tissue engineering strategies, mainly because they mimic, in some way, the complexity and nanoscale detail observed in real organs. The chondrogenic potential of these scaffolds was already demonstrated, but their osteogenic potential is not corroborated yet. In order to assess if a hierarchical ...