14 documents found, page 1 of 2
Characterisation of Films Based on Exopolysaccharides from Alteromonas Strains ...
Concórdio-Reis, Patrícia; Pereira, João R.; Delgado Alves, Vitor; Nabais, Ana R.; Neves, Luísa A.; Marques, Ana C.; Fortunato, Elvira; Moppert, XavierThis work assessed the film-forming capacity of exopolysaccharides (EPS) produced by six Alteromonas strains recently isolated from different marine environments in French Polynesia atolls. The films were transparent and resulted in small colour alterations when applied over a coloured surface (ΔEab below 12.6 in the five different colours tested). Moreover, scanning electron microscopy showed that the EPS film...
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlorora...
Pereira, João R.; Araújo, Diana; Freitas, Patrícia; Marques, Ana C.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Fortunato, ElviraPseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol...
Cation-mediated gelation of the fucose-rich polysaccharide FucoPol: preparation...
Fialho, Letícia; Araújo, Diana; Delgado Alves, Vitor; Roma-Rodrigues, Catarina; Baptista, Pedro V.; Fernandes, Alexandra R.; Freitas, FilomenaThis study describes for the first time the iron- and copper-mediated gelation of FucoPol, fucoserich bacterial polysaccharide. The ability of FucoPol to gel in the presence of metal cations, including iron(III) and copper(II), was used for the preparation of hydrogel beads. Iron mediated the formation of stable and not cytotoxic gel beads, while copper resulted in fragile and cytotoxic ones. Copper-mediated be...
Characterization and biotechnological potential of extracellular polysaccharide...
Concórdio-Reis, Patrícia; Delgado Alves, Vitor; Moppert, Xavier; Guezennec, Jean; Freitas, Filomena; Reis, Maria A.M.Marine environments comprise almost three quarters of Earth’s surface, representing the largest ecosystem of our planet. The vast ecological and metabolic diversity found in marine microorganisms suggest that these marine resources have a huge potential as sources of novel commercially appealing biomolecules, such as exopolysaccharides (EPS). Six Alteromonas strains from different marine environments in French ...
Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization...
Araújo, Diana; Delgado Alves, Vitor; Marques, Ana C.; Fortunato, Elvira; Reis, Maria A.M.; Freitas, FilomenaChitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first...
Low temperature dissolution of yeast chitin-glucan complex and characterization...
Araújo, Diana; Delgado Alves, Vitor; Marques, Ana C.; Fortunato, Elvira; Reis, Maria A.M.; Freitas, FilomenaChitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first...
Preparation and characterization of films based on a natural P(3HB)/mcl-PHA ble...
Rebocho, Ana Teresa; Pereira, João R.; Neves, Luísa A.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Freitas, Filomena; Reis, Maria A.M.The co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed...
Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Ble...
Rebocho, Ana Teresa; Pereira, João R.; Neves, Luísa A.; Delgado Alves, Vitor; Sevrin, Chantal; Grandfils, Christian; Freitas, Filomena; Reis, Maria A.M.The co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed...
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safe...
Araújo, Diana; Delgado Alves, Vitor; Lima, Sofia A.C.; Reis, Salette; Freitas, Filomena; Reis, Maria A.M.Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding ...
Conversion of cheese whey into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by ...
Pais, Joana; Serafim, Luísa S.; Freitas, Filomena; Reis, Maria A.M.Haloferax mediterranei was cultivated in highly saline medium using cheese whey as the substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV). Acid hydrolysis provided a simple inexpensive method to obtain a cheese whey hydrolysate that was used for cultivation of H. mediterranei. Batch bioreactor cultivation of H. mediterranei resulted in the production of an active biomass...