9 documents found, page 1 of 1
Paving the way for a non‐antibiotic and microbiota friendly therapy for Helicob...
Seabra, CL; Pinho, AS; Nunes, C; Amorim, I; Pedro, N; Henriques, P; Monteiro, C; Gomes, J; Machado, C; Gartner, F; Pereira, L; Reis, S; Reis, CABackground: The World Health Organization has identified Helicobacter pylori, a Gram-negative bacterium responsible for several gastric disorders, as one of the pathogenic bacteria that requires newer non-antibiotic approaches for its management. We previously demonstrated that nanostructured lipid carriers (NLC) loaded with docosahexaenoic acid (DHA-NLC)have excellent in vitro performance against H. pylori. Ma...
Presentations of Children to Emergency Departments Across Europe and the COVID-...
Nijman, RG; Honeyford, K; Farrugia, R; Rose, K; Bognar, Z; Buonsenso, D; Da Dalt, L; De, T; Maconochie, IK; Parri, N; Roland, D; Alfven, T; Aupiais, CBackground: During the initial phase of the Coronavirus Disease 2019 (COVID-19) pandemic, reduced numbers of acutely ill or injured children presented to emergency departments (EDs). Concerns were raised about the potential for delayed and more severe presentations and an increase in diagnoses such as diabetic ketoacidosis and mental health issues. This multinational observational study aimed to study the numbe...
Feasibility and acceptability of psychosocial care forunsuccessful fertility tr...
Sousa-Leite, M; Fernandes, M; Reis, S; Costa, R; Figueiredo, B; Gameiro, SIntroduction Many people undergo fertility treatment to have biological children, but around four in ten patients complete all treatment cycles without having the children they desire. This triggers intense grief from which patients report taking on average 2 years to recover. Fertility guidelines and regulators stress the need to support patients through this process, but there is a scarcity of evaluated inter...
How Charge, Size and Protein Corona Modulate the Specific Activity of Nanostruc...
Chitas, R; Nunes, C; Reis, S; Parreira, P; Martins, MCLThe major risk factor associated with the development of gastric cancer is chronic infection with Helicobacter pylori. The available treatments, based on a cocktail of antibiotics, fail in up to 40% of patients and disrupt their gut microbiota. The potential of blank nanostructured lipid carriers (NLC) for H. pylori eradication was previously demonstrated by us. However, the effect of NLC charge, size and prote...
Evaluation of the antitumour and antiproliferative effect of xanthohumol-loaded...
Fonseca, M; Macedo, AS; Lima, SAC; Reis, S; Soares, R; Fonte, PCutaneous melanoma is the deadliest type of skin cancer and current treatment is still inadequate, with low patient survival rates. The polyphenol xanthohumol has been shown to inhibit tumourigenesis and metastasization, however its physicochemical properties restrict its application. In this work, we developed PLGA nanoparticles encapsulating xanthohumol and tested its antipro-liferative, antitumour, and migra...
A selective p53 activator and anticancer agent to improve colorectal cancer the...
Ramos, H; Soares, MIL; Silva, J; Raimundo, L; Calheiros, J; Gomes, C; Reis, F; Monteiro, FA; Nunes, C; Reis, S; Bosco, B; Piazza, S; Domingues, LImpairment of the p53 pathway is a critical event in cancer. Therefore, reestablishing p53 activity has become one of the most appealing anticancer therapeutic strategies. Here, we disclose the p53-activating anticancer drug (3S)-6,7-bis(hydroxymethyl)-5-methyl-3-phenyl-1H,3H-pyrrolo[1,2-c]thiazole (MANIO). MANIO demonstrates a notable selectivity to the p53 pathway, activating wild-type (WT)p53 and restoring W...
Optimization of rifapentine-loaded lipid nanoparticles using a Quality-by-Desig...
Magalhães, J; Chaves, LL; Vieira, AC; Santos, SG; Pinheiro, M; Reis, SThis work aims to optimize and assess the potential use of lipid nanoparticles, namely nanostructured lipid carriers (NLCs), as drug delivery systems of rifapentine (RPT) for the treatment of tuberculosis (TB). A Box-Behnken design was used to increase drug encapsulation efficiency (EE) and loading capacity (LC) of RPT-loaded NLCs. The optimized nanoparticles were fully characterized, and their effect on cell v...
Delivering amoxicillin at the infection site-a rational design through lipid na...
Lopes-de-Campos, D; Pinto, RM; Lima, SAC; Santos, T; Sarmento, B; Nunes, C; Reis, SPurpose: Amoxicillin is a commonly used antibiotic, although degraded by the acidic pH of the stomach. This is an important limitation for the treatment of Helicobacter pylori infections. The purpose of this work was to encapsulate amoxicillin in lipid nanoparticles, increasing the retention time at the site of infection (gastric mucosa), while protecting the drug from the harsh conditions of the stomach lumen....
Mannosylated solid lipid nanoparticles for the selective delivery of rifampicin...
Vieira, A; Chaves, L; Pinheiro, M; Lima, S; Ferreira, D; Sarmento, B; Reis, STuberculosis (TB) is still a devastating disease and more people have died of TB than any other infectious diseases throughout the history. The current therapy consists of a multidrug combination in a long-term treatment, being associated with the appearance of several adverse effects. Thus, solid lipid nanoparticles (SLNs) were developed using mannose as a lectin receptor ligand conjugate for macrophage target...