11 documents found, page 1 of 2
Astrocyte-derived TNF and glutamate critically modulate microglia activation by...
Canedo, T; Portugal, CC; Socodato, R; Almeida, TO; Terceiro, AF; Bravo, J; Silva, AI; Magalhães, JD; Guerra-Gomes, S; Oliveira, JF; Sousa, NMethamphetamine (Meth) is a powerful illicit psychostimulant, widely used for recreational purposes. Besides disrupting the monoaminergic system and promoting oxidative brain damage, Meth also causes neuroinflammation, contributing to synaptic dysfunction and behavioral deficits. Aberrant activation of microglia, the largest myeloid cell population in the brain, is a common feature in neurological disorders tri...
A Protocol for FRET-Based Live-Cell Imaging in Microglia
Socodato, R; Melo, PN; Ferraz-Nogueira, JP; Portugal, CC; Relvas, JBThis protocol highlights the use of FRET-based biosensors to investigate signaling events during microglia activation in real time. Understanding microglia activation has gained momentum as it can help decipher signaling mechanisms underlying the neurodegenerative process occurring in neurological disorders. Unlike more traditional methods widely employed in the microglia field, FRET allows microglia signaling ...
Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss...
Socodato, R; Henriques, JF; Portugal, CC; Almeida, TO; Tedim-Moreira, J; Alves, RL; Canedo, T; Silva, C; Magalhães, A; Summavielle, T; Relvas, JBAlcohol abuse adversely affects the lives of millions of people worldwide. Deficits in synaptic transmission and in microglial function are commonly found in human alcohol abusers and in animal models of alcohol intoxication. Here, we found that a protocol simulating chronic binge drinking in male mice resulted in aberrant synaptic pruning and substantial loss of excitatory synapses in the prefrontal cortex, wh...
The antioxidant peptide salamandrin-i: First bioactive peptide identified from ...
Plácido, A; Bueno, J; Barbosa, EA; Moreira, DC; Dias, JDN; Cabral, WF; Albuquerque, P; Bessa, LJ; Freitas, J; Kuckelhaus, SAS; Lima, FCDAAmphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, sal...
Microglia Dysfunction Caused by the Loss of Rhoa Disrupts Neuronal Physiology a...
Socodato, R; Portugal, CC; Canedo, T; Rodrigues, A; Almeida, TO; Henriques, JF; Vaz, SH; Magalhães, J; Silva, CM; Baptista, FI; Alves, RLNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of ß-amyloid plaques, and memory ...
Novel Ocellatin Peptides Mitigate LPS-induced ROS Formation and NF-kB Activatio...
Sousa, NA; Oliveira, GAL; Oliveira, AP; Lopes, ALF; Iles, B; Nogueira, KM; Araújo, TSL; Souza, LKM; Araújo, AR; Ramos-Jesus, J; Plácido, A; Amaral, CCutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from the cutaneous secretions of the amphibian, Leptodactylus vastus, as a source of bioactive molecules. The peptides obtained possessed the amino acid sequences, GVVDILKGAAKDLAGH and GVV...
Collar occupancy: a new quantitative imaging tool for morphometric analysis of ...
Nova, FB; Maia, AF; Cruz, A; Millar, V; Pinto, IM; Relvas, JB; Domingues, HS"Background Oligodendrocytes (OL) are the myelinating cells of the central nervous system. OL differentiation from oligodendrocyte progenitor cells (OPC) is accompanied by characteristic stereotypical morphological changes. Quantitative imaging of those morphological alterations during OPC differentiation is commonly used for characterization of new molecules in cell differentiation and myelination and screenin...
Emerging biosensing technologies for neuroinflammatory and neurodegenerative di...
Abreu, C; Soares-Dos-Reis, R; Melo, PN; Relvas, JB; Guimarães, J; Sá, M; Cruz, AP; Pinto, INeuroinflammation plays a critical role in the onset and progression of many neurological disorders, including Multiple Sclerosis, Alzheimer’s and Parkinson’s diseases. In these clinical conditions the underlying neuroinflammatory processes are significantly heterogeneous. Nevertheless, a common link is the chronic activation of innate immune responses and imbalanced secretion of pro and anti-inflammatory media...
Caveolin-1-mediated internalization of the vitamin C transporter SVCT2 in micro...
Portugal, CC; Socodato, R; Canedo, T; Silva, CM; Martins, T; Coreixas, VS; Loiola, EC; Gess, B; Röhr, D; Santiago, AR; Young, P; Minshall, RDVitamin C is essential for the development and function of the central nervous system (CNS). The plasma membrane sodium-vitamin C cotransporter 2 (SVCT2) is the primary mediator of vitamin C uptake in neurons. SVCT2 specifically transports ascorbate, the reduced form of vitamin C, which acts as a reducing agent. We demonstrated that ascorbate uptake through SVCT2 was critical for the homeostasis of microglia, t...
Modulation of oligodendrocyte differentiation and maturation by combined bioche...
Lourenço, T; Faria, JP; Bippes, C; Maia, J; Lopes-Da-Silva, J; Relvas, JB; Graõs, MExtracellular matrix (ECM) proteins play a key role during oligodendrogenesis. While fibronectin (FN) is involved in the maintenance and proliferation of oligodendrocyte progenitor cells (OPCs), merosin (MN) promotes differentiation into oligodendrocytes (OLs). Mechanical properties of the ECM also seem to affect OL differentiation, hence this study aimed to clarify the impact of combined biophysical and bioche...