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Synthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis na...
Brito, Rory Cristiane Fortes de; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Mathias, Fernando Augusto SiqueiraReverse vaccinology or immunoinformatics is a computational methodology which integrates data from in silico epitope prediction, associated to other important information as, for example, the predicted subcellular location of the proteins used in the design of the context of vaccine development. This approach has the potential to search for new targets for vaccine development in the predicted proteome of pathog...
Peptide vaccines for leishmaniasis.
Brito, Rory Cristiane Fortes de; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Vieira, João Filipe PereiraDue to an increase in the incidence of leishmaniases worldwide, the development of new strategies such as prophylactic vaccines to prevent infection and decrease the disease have become a high priority. Classic vaccines against leishmaniases were based on live or attenuated parasites or their subunits. Nevertheless, the use of whole parasite or their subunits for vaccine production has numerous disadvantages. T...
An assessment on epitope prediction methods for protozoa genomes.
Resende, Daniela de Melo; Rezende, Antonio Mauro; Oliveira, Nesley Jesus Daher de; Batista, Izabella Cristina Andrade; Oliveira, Rodrigo Corrêa deBackground: Epitope prediction using computational methods represents one of the most promising approaches to vaccine development. Reduction of time, cost, and the availability of completely sequenced genomes are key points and highly motivating regarding the use of reverse vaccinology. Parasites of genus Leishmania are widely spread and they are the etiologic agents of leishmaniasis. Currently, there is no eff...
Immunoinformatics features linked to leishmania vaccine development : data inte...
Brito, Rory Cristiane Fortes de; Guimarães, Frederico Gonçalves; Velloso, João Paulo Linhares; Oliveira, Rodrigo Corrêa de; Ruiz, Jeronimo ConceiçãoLeishmaniasis is a wide-spectrum disease caused by parasites from Leishmania genus. There is no human vaccine available and it is considered by many studies as apotential effective tool for disease control. To discover novel antigens, computational programs have been used in reverse vaccinology strategies. In this work, we developed a validation antigen approach that integrates prediction of B and T cell epitop...
Resposta celular em linfonodos de bovinos inoculados com Anaplasma marginale
Resende, Daniela de MeloFundação de Amparo a Pesquisa do Estado de Minas Gerais; The immune response against the pathogen Anaplasma marginale was evaluated after the inoculation, in calves, with the strain AUFV1 2nd passage. For this, routine coloration techniques and immunohistochemistry techniques were performed in slides of superficial limph nodes. In the first week post inoculation, a proliferative response was observed in the par...
Molecular diagnosis of canine visceral leishmaniasis : a comparative study of t...
Reis, Levi Eduardo Soares; Vital, Wendel Coura; Roatt, Bruno Mendes; Bouillet, Leoneide Érica Maduro; Ker, Henrique GamaPolymerase chain reaction (PCR) and its variations represent highly sensitive and specificmethods for Leishmania DNA detection and subsequent canine visceral leishmaniasis (CVL)diagnosis. The aim of this work was to compare three different molecular diagnosis tech-niques (conventional PCR [cPCR], seminested PCR [snPCR], and quantitative PCR [qPCR])in samples of skin and spleen from 60 seropositive dogs by immun...
Epitope mapping and protective immunity elicited by adenovirus expressing the L...
Resende, Daniela de Melo; Caetano, Bráulia Costa; Dutra, Míriam Santos; Abrantes, Christiane de Freitas; Verly, Rodrigo MoreiraA2was identified as an amastigote virulence factor of Leishmania (Leishmania) donovani and as a candidate antigen for vaccine development against visceral leishmaniasis. Here, predicted hydrophilic, class I and II MHC-binding synthetic peptides were used to define epitopes recognized by A2-specific antibodies, CD8+ T and CD4+ T cells, respectively. Immunization of BALB/c mice with adenovirus expressing A2 (AdA2...
Evidence for Reductive Genome Evolution and Lateral Acquisition of Virulence Fu...
Ruiz, Jerônimo Conceição; Ferreira, Vívian D'Afonseca da Silva; Silva, Artur Luiz da Costa da; Ali, Amjad; Pinto, Anne Cybellep. 1-15; Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-02-27T13:03:23Z No. of bitstreams: 1 journal.pone.0018551.pdf: 410957 bytes, checksum: 9150a5f0fbe637d4f1197a80e401c8d2 (MD5); Made available in DSpace on 2012-02-27T13:03:23Z (GMT). No. of bitstreams: 1 journal.pone.0018551.pdf: 410957 bytes, checksum: 9150a5f0fbe637d4f1197a80e401c8d2 (MD5) Previous issue date: 2011; Background: Coryneb...