6 documents found, page 1 of 1
Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α i...
Tonelli, Fernanda MP; dos Santos NassifLacerda, Samyra Maria; Procópio, Marcela Santos; Lemos, Breno Luiz Sales; França, Luiz Renato deMicroinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transducti...
Mechanisms of neural differentiation and integration [Editorial]
Resende, Rodrigo Ribeiro
Studying complex system: calcium oscillations as attractor of cell differentiation
Resende, Rodrigo Ribeiro
Human adult stem cells from diverse origins: an overview from multiparametric i...
Resende, Rodrigo Ribeiro
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effect...
Dutra, Alexandre Augusto de Assis; Sousa, Lucas Oliveira; Resende, Rodrigo Ribeiro; Brandão, Rogélio Lopes; Kalapothakis, EvanguedesHere, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LT...