18 documents found, page 1 of 2
Identification of novel carboxylate transporters encoded in the Cyberlindnera j...
Sousa-Silva, M.; Ribas, David Manuel Nogueira; Casal, Margarida; Soares-Silva, Isabel JoãoNovel strategies to boost bio-based production of organic acids are focused in the expression of specific transporter proteins, to improve adequate uptake and export mechanisms. This study focused on the identification and characterization of novel carboxylate (CA) transporters in the yeast Cyberlindnera jadinii. Transporters homologous to Jen1p and Ady2p, the lactate and acetate permeases from Saccharomyces ce...
Engineering of AceTr membrane transporters to improve organic acid production i...
Rendulic, Toni; Silva, João Pedro Azevedo; Ribas, David Manuel Nogueira; Castagnoli, Davide; Paiva, Sandra; Soares-Silva, Isabel João; Nevoigt, ElkeOrganic acids are industrially relevant chemicals obtainable from renewable feedstocks via microbial cell factories. Microbially produced organic acids have a wide variety of applications, including bioplastic synthesis. Thus, they possess the potential to replace petroleum-derived commodity chemicals that are obtained through unsustainable production processes. Yeasts commonly represent the organisms of choice...
Characterization of novel plasma membrane carboxylate transporters from non-con...
Senra, Emanuel Sousa; Sousa-Silva, M.; Ribas, David Manuel Nogueira; Casal, Margarida; Soares-Silva, Isabel JoãoCarboxylic acids (CAs) are a group of organic compounds that play a central role in cellular metabolism of many organisms, using it as unique sources of carbon and energy [1]. In order to replace conventional petroleum-based methods for the obtainment of CAs, alternatives are required for more sustainable way of producing these compounds. The exploitation of yeast biodiversity has received great interest from f...
Characterization of Cyberlindnera jadinii carboxylate permeases by heterologous...
Sousa-Silva, M.; Ribas, David Manuel Nogueira; Casal, Margarida; Soares-Silva, Isabel JoãoBackground: The wide applicability of organic acids for direct use as commodity chemicals and as polymer building blocks has evidenced their importance in diverse types of industries. In Saccharomyces cerevisiae, two permeases are responsible for the uptake of carboxylates (CA) at the plasma membrane, Jen1p a monocarboxylate proton symporter (Major Facilitator Superfamily) and Ady2p an acetate permease (AceTr F...
Characterization of Cyberlindnera jadinii carboxylate transporters by heterolog...
Sousa-Silva, M.; Ribas, David Manuel Nogueira; Casal, Margarida; Soares-Silva, Isabel JoãoConcerning the global problems of resource scarcity and environmental damage, new technologies based on renewable biological sources are needed as the current model of natural resource exploitation is unsustainable. Cell factories with specific genetic and physiological traits, namely suitable protein transporters, may be key players in the bio-based production of organic acids, as an alternative approach to th...
Exploring the Cyberlindnera jadinii yeast transportome to uncover novel carboxy...
Sousa-Silva, M.; Vieira, Daniel; Ribas, David Manuel Nogueira; Soares, Pedro; Casal, Margarida; Soares-Silva, Isabel JoãoDue to global problems of resource scarcity and environmental damage, new technologies based on renewable biological sources are required, as the current model of natural resource exploitation is unsustainable. Novel strategies to boost bio-based production of organic acids are based on the expression of carboxylate transporters in microbial cell factories. In this work we have focused on the identification and...
Screening of non-Saccharomyces wild yeasts for biotechnological applications
Sousa-Silva, M.; Ribas, David Manuel Nogueira; Franco-Duarte, Ricardo; Pais, Célia; Casal, Margarida; Soares-Silva, Isabel JoãoIn nature, a wide diversity of yeast species can be found even in wastes from food industries. The exploration of this yeast biodiversity has captured great interest from food, pharmaceutical and even fuel companies due to the interesting properties of such microorganisms [1]. These microorganisms can transform sugars present in raw materials into different valuable compounds as several chemical building-blocks...
Exploring the Cyberlindnera jadinii transportome for the identification of nove...
Sousa-Silva, M.; Vieira, Daniel; Ribas, David Manuel Nogueira; Soares, Pedro; Casal, Margarida; Soares-Silva, Isabel JoãoConsidering the global problems of resource scarcity and environmental damage, new technologies based on renewable biological sources are needed as current model of natural resource exploitation is unsustainable. Novel strategies to boost bio-based production of organic acids are based on the expression of carboxylate transporters in microbial cell factories. In this work we have focused on the identification a...
Exploring plasma membrane transporters to improve organic acid production in ye...
Rendulic, Toni; Silva, João Pedro Azevedo; Xiberras, Joeline; Medonça Bahia, Frederico; Ribas, David Manuel Nogueira; Castagnoli, Davide; Paiva, SandraOrganic acids are industrially relevant building-block chemicals obtainable from renewable feedstocks by utilization of microbial cell factories. With a wide variety of applications, including bioplastics synthesis, microbially produced organic acids have the potential to replace petroleum-derived commodity chemicals that are obtained through unsustainable production processes. Yeasts commonly represent the org...
Functional characterization of Cyberlindnera jadinii carboxylate transporters i...
Sousa-Silva, M.; Ribas, David Manuel Nogueira; Casal, Margarida; Soares-Silva, Isabel JoãoIntroduction In Saccharomyces cerevisiae, two permeases are responsible for the uptake of carboxylates (CA) at the plasma membrane, Jen1p a monocarboxylate proton symporter (Major Facilitator Superfamily) and Ady2p an acetate permease (AceTr Family). In Cyberlindnera jadinii, different uptake systems for CAs were functionally characterized however until now the genes encoding these transporters remain unidentif...