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Genomic analysis of Sinorhizobium meliloti IRAMC:0087 an halotolerant rhizobium...

BenGaied, R; Sbissi, I; Brígido, C; Tarhouni, M

Arid and Saharan regions of Tunisia have great interest due to their endemic spontaneous legume’s diversity. The strain IRAMC:0087 was isolated from root nodules of a Saharan shrub Genista saharae growing in Southern Tunisia. Phenotypic characterization of this strain has revealed tolerance to high salinity levels, drought and high temperatures. To investigate the basis of this, we sequenced its complete genome...


Evaluating the effect of various bacterial consortia isolated from arid wild le...

BenGaied, R; Brígido, C; Sbissi, I; Tarhouni, M

High-temperature stress affects the growth and developmental process of cool-season grain legumes. We hypothesized that endophytic bacteria associated with arid plants could be a potential resource to ensure the tolerance of cold-season legumes to high temperature stress events. To test our hypothesis, Phyllobacterium salinitolerans (PH), Starkeya sp. (ST) and Pseudomonas turukhanskensis (PS) endophytes of diff...


Genomic characteristics and comparative genomics analyses of non-rhizobial endo...

Torres, DP; Alexandre, A; Menendez, E; Brígido, C

In addition to typical nitrogen-fixing endosymbionts, legumes harbour other endophytic bacteria within their tissues that may also contribute to plant growth and health (Hardoim et al. 2015). Our previous works revealed that endophytic bacteria isolated from legumes not only have plant growth-promoting traits but are also well adapted to common constraints present in soils of the Mediterranean region (Brígido e...


Dupla inoculação (rizóbio e fungos micorrízicos arbusculares) mitigam efeitos n...

Leal, VC; Brígido, C; Souza, IS; Lacerda Júnior, GV; Carvalho, M; Fernandes-Júnior, PI; Martins, LMV

A salinidade em áreas áridas e semiáridas do mundo está a expandir rapidamente devido às alterações climáticas e atividades humanas. O uso de inóculos contendo microrganismos benéficos, como fungos micorrízicos arbusculares (FMA) e bactérias simbióticas fixadoras de azoto, aliado ao manejo adequado do solo e das culturas, é uma alternativa promissora para melhorar a produção vegetal nessas regiões. Aqui, invest...


Endophytic bacteria associated with spontaneous legumes in arid zones of Tunisi...

BenGaied, R; Brígido, C; Sbissi, I; Tarhouni, M

Legumes naturally adapted to harsh climate environments represent a new source of plant growth promoting (PGP) bacteria that can be used to improve crop resilience to climate change. However, the diversity and functionality of endophytic bacteria associated with endemic wild legumes in Tunisia are almost unknown. To study the taxonomic and functional diversity of these species, we conducted a study on root nodu...


Introduction of exogenous AMF species alters the biological diversity and funct...

Leal, VC; Brígido, C; Souza, IJS; Lacerda Júnior, GV; Carvalho, M; Fernandes-Júnior, PI; Martins, LMV

The salinity in arid and semi-arid areas of the world is rapidly expanding due to climate change and anthropogenic activities. The use of inoculants containing beneficial microbes (e.g. arbuscular mycorrhizal fungi (AMF) and rhizobia) is a promising alternative to improve plant production in these regions. Here, we investigated the effect of common agricultural practices such as the use of beneficial microbes a...


Role of QseG membrane protein in beneficial enterobacterial interactions with p...

Brígido, C; Torres, Denise P.; Paço, A.; Menéndez, E.; Mateos, P.F.

Membrane protein Quorum sensing G (QseG) positively interferes in the process of colonization and infection of enteric pathogens in animals. Its gene is located between qseE and qseF genes and is co-transcribed with the two-component system. Homologs of qseG gene, along with qseEF, are present in many Enterobacteriaceae; however, its role in nonpathogenic strains is still unknown. To fill this knowledge gap, we...


Strategy to manage AMF biological diversity within the cropping system

Brígido, C; van Tuinen, D; Brito, I; Alho, L; Goss, MJ; Carvalho, M

Despite the great biological diversity of arbuscular mycorrhizal fungi (AMF) and its functional consequences on the host plant, a strategy to manage this diversity within the cropping system is still missing. The importance of this strategy is reinforced by the fact that commercial inocula are too expensive to be used in a large scale and only contain a single or few AMF species. When high levels of botanical h...


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