11 documents found, page 1 of 2
Insights into the anticancer activity of chiral alkylidene-β-lactams and alkyli...
Alves, Américo J. S.; Alves, Nuno G.; Laranjo, Mafalda; Gomes, Clara S. B.; Gonçalves, Ana Cristina; Ribeiro, Ana Bela Sarmento; Botelho, M. FilomenaChiral alkylidene-β-lactams and alkylidene-γ-lactams were synthesized and screened for their in vitro activity against four human cancer cell lines (melanoma, esophageal, lung and fibrosarcoma carcinoma). Alkylidene-β-lactams were synthesized via Wittig reaction of diverse phosphorus ylides with benzhydryl 6-oxopenicillanate, derived from 6-aminopenicillanic acid. Moreover, novel chiral alkylidene-γ-lactams wer...
DNA Methylation Is Correlated with Oxidative Stress in Myelodysplastic Syndrome...
Gonçalves, Ana Cristina; Alves, Raquel; Baldeiras, Inês; Marques, Bárbara; Oliveiros, Bárbara; Pereira, Amélia; Costa, José Manuel NascimentoOxidative stress and abnormal DNA methylation have been implicated in cancer, including myelodysplastic syndromes (MDSs). This fact leads us to investigate whether oxidative stress is correlated with localized and global DNA methylations in the peripheral blood of MDS patients. Sixty-six MDS patients and 26 healthy individuals were analyzed. Several oxidative stress and macromolecule damage parameters were anal...
Effects of Adper™ Scotchbond™ 1 XT, Clearfil™ SE Bond 2 and Scotchbond™ Univers...
Cardoso, Miguel; Coelho, Ana; Marto, Carlos Miguel; Gonçalves, Ana Cristina; Paula, Anabela; Ribeiro, Ana Bela Sarmento; Ferreira, Manuel MarquesThis study aimed to assess the cytotoxicity of commercially available adhesive strategies-etch-and-rinse (Adper™ Scotchbond™ 1 XT, 3M ESPE, St. Paul, MN, USA, SB1), self-etch (Clearfil™ SE Bond 2, Kuraray Noritake Dental Inc., Tokyo, Japan, CSE), and universal (Scotchbond™ Universal, 3M Deutschland GmbH, Neuss, Germany, SBU). MDPC-23 cells were exposed to adhesives extracts in different concentrations and expos...
Resistance to tyrosine kinase inhibitors in chronic myeloid leukemia — from mol...
Alves, Raquel; Gonçalves, Ana Cristina; Rutella, Sergio; Almeida, António M.; Rivas, Javier De Las; Trougakos, Ioannis P.; Ribeiro, Ana Bela SarmentoResistance to targeted therapies is a complex and multifactorial process that culminates in the selection of a cancer clone with the ability to evade treatment. Chronic myeloid leukemia (CML) was the first malignancy recognized to be associated with a genetic alteration, the t(9;22)(q34;q11). This translocation originates the BCR-ABL1 fusion gene, encoding the cytoplasmic chimeric BCR-ABL1 protein that displays...
Flow cytometry and targeted immune transcriptomics identify distinct profiles i...
Alves, Raquel; McArdle, Stephanie E B; Vadakekolathu, Jayakumar; Gonçalves, Ana Cristina; Tavares, Paulo; Pereira, Amélia; Almeida, Antonio M.Background: Tumor cells have evolved complex strategies to escape immune surveillance, a process which involves NK cells and T lymphocytes, and various immunological factors. Indeed, tumor cells recruit immunosuppressive cells [including regulatory T-cells (Treg), myeloid-derived suppressor cells (MDSC)] and express factors such as PD-L1. Molecularly targeted therapies, such as imatinib, have off-target effects...
Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast...
Paula, Anabela; Laranjo, Mafalda; Marto, Carlos Miguel; Abrantes, Ana Margarida; Casalta-Lopes, João; Gonçalves, Ana Cristina(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mous...
Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonat...
Paulo, Siri; Laranjo, Mafalda; Abrantes, Ana M.; Casalta-Lopes, João; Santos, Kathleen; Gonçalves, Ana C.; Paula, Anabela Baptista; Marto, Carlos Miguel(1) Background: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is one of the most often seen side effects in patients treated with nitrogen-containing bisphosphonates (BPs), a post-surgical non-healing wound condition. Since calcium phosphate (CP) compounds are able to adsorb zoledronate (ZOL) when used as a drug delivery vehicle, we aimed to verify if these ceramics might have a potential protective e...
MicroRNA signature refine response prediction in CML
Alves, Raquel; Gonçalves, Ana Cristina; Jorge, Joana; Marques, Gilberto João Padilha; Luís, Dino; Ribeiro, André B.; Tavares, Paulo; Oliveiros, BárbaramicroRNAs (miRs) dysregulation have emerged as a crucial step in tumorigenesis, being related with cancer development, progression and response to treatment. In chronic myeloid leukaemia (CML), the resistance to tyrosine kinase inhibitors (TKI) is responsible for treatment failure and could be linked to changes in miRs expression. This work aimed to correlate the expression levels of 3 miRs, miR-21, miR-26b and...
Intestinal inflammatory and redox responses to the perioperative administration...
Costa, Beatriz Pinto; Gonçalves, Ana Cristina; Abrantes, Ana M.; Matafome, Paulo N.; Seiça, Raquel; Ribeiro, Ana Bela Sarmento; Botelho, Maria FilomenaPurpose: To investigate the inflammatory and redox responses to teduglutide on an animal model of laparotomy and intestinal anastomosis. Methods: Wistar rats (n=62) were allocated into four groups: “Ileal Resection and Anastomosis” vs. “Laparotomy”, each one split into “Postoperative Teduglutide Administration” vs. “No Treatment”; and euthanized at the third or the seventh day. Ileal and blood samples were reco...
Impact of splenic artery ligation after major hepatectomy on liver function, re...
Carrapita, Jorge; Abrantes, Ana Margarida; Campelos, Sofia; Gonçalves, Ana Cristina; Cardoso, Dulce; Ribeiro, Ana Bela Sarmento; Rocha, ClaraIt was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluat...