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Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integr...

Matias, João; Rodrigues, Thomas; Torres, Cristiana A. V.; Marques, Susana; Ribeiro, Belina; Gírio, Francisco; Reis, Maria A.; Freitas, Filomena

ABSTRACT: This study integrates the valorization of a lignocellulose material into poly(3-hydroxybutyrate), P(3HB), with biopolymer extraction from bacterial cells with the enzyme alcalase. The work focused on Burkholderia thailandensis DSM 13276 as the P(3HB) producer and on eucalyptus bark, a byproduct from the pulp industry, as the sole feedstock for bacterial cultivation. The eucalyptus bark was hydrolyzed ...

Date: 2026   |   Origin: Repositório do LNEG

Monitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytom...

Lopes da Silva, Teresa; Silva, Thiago; França, Bruna Thomazinho; Ribeiro, Belina; Reis, Alberto

ABSTRACT: Winery wastewater (WWW), if released untreated, poses a serious environmental threat due to its high organic load. In this study, Chlorella vulgaris was cultivated in diluted WWW to assess its suitability as a culture medium. Two outdoor cultivation systems-a 270 L raceway and a 40 L bubble column-were operated over 33 days using synthetic medium (control) and WWW. A flow cytometry (FC) protocol was i...

Date: 2025   |   Origin: Repositório do LNEG

Assessment of piggery wastewater treatment in vertical flow constructed wetland...

de Oliveira Corrêa, Diego; Ferreira, Alice; Ribeiro, Belina; Gogoi, Jayanta; Karan, N.; Nalwad, A.; Ganguly, A.; Mutnuri, S.; Gouveia, Luisa

ABSTRACT: Piggery wastewater (PWW), rich in pollutants, poses significant environmental risks if not properly treated. Natural treatment processes, such as constructed wetlands (combined action of plants, substrates, and microbes) and microalgae cultivation, offer sustainable and low-cost alternatives for managing these effluents while enabling resource recovery. This study represents an initial step toward opt...

Date: 2025   |   Origin: Repositório do LNEG

Innovative approach in sustainable agriculture: Harnessing microalgae potential...

Ferreira, Alice; Vladic, Jelena; Corrêa, D. O.; Butzke, Valéria Louzada Leal; Martins, Pedro L.; Ribeiro, Belina; Santos, Cláudia Marques dos

ABSTRACT: Microalgae can contribute to sustainable agriculture and wastewater treatment. This study investigated Tetradesmus obliquus, grown in piggery wastewater (To-PWW), as a biostimulant/biofertilizer compared to biomass grown in synthetic medium (To-B). Subcritical water extraction was tested for disruption/hydrolysis of wet biomass, at three temperatures (120, 170, and 220 °C) and two biomass loads (1:10 ...

Date: 2024   |   Origin: Repositório do LNEG

Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, D. O.; Ribeiro, Belina; Silva, Teresa Lopes da; Santos, Cláudia Marques dos; Acién, F. Gabriel; Gouveia, Luisa

ABSTRACT: In the present work, two downstream processes - high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) - were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination),...

Date: 2024   |   Origin: Repositório do LNEG

Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, Diego O.; Ribeiro, Belina; Silva, Teresa Lopes da; Marques-dos-Santos, Cláudia; Acién, F. Gabriel; Gouveia, Luisa

In the present work, two downstream processes - high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) - were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination), mung bean...


Bioprocess to produce biostimulants/biofertilizers based on microalgae grown us...

Ferreira, Alice; Corrêa, Diego O.; Ribeiro, Belina; Silva, Teresa Lopes da; Marques-dos-Santos, Cláudia; Gabriel Acién, F.; Gouveia, Luisa

In the present work, two downstream processes − high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) − were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination), mung bean...


Innovative approach in sustainable agriculture: harnessing microalgae potential...

Ferreira, Alice; Vladić, Jelena; Corrêa, Diego de Oliveira; Butzke, Valéria Louzada Leal; Martins, Pedro L.; Ribeiro, Belina

Microalgae can contribute to sustainable agriculture and wastewater treatment. This study investigated Tetradesmus obliquus, , grown in piggery wastewater (To-PWW), as a biostimulant/ biofertilizer compared to biomass grown in synthetic medium (To-B). Subcritical water extraction was tested for disruption/hydrolysis of wet biomass, at three temperatures (120, 170, and 220 degrees C) and two biomass loads (1:10 ...


From piggery wastewater to wheat using microalgae towards zero waste

Ferreira, Alice; Figueiredo, Daniel; Ferreira, Francisca; Marujo, Ana; Bastos, Carolina R. V.; Martin-Atanes, Guillermo; Ribeiro, Belina

ABSTRACT: Microalgae production is still expensive, driving the need to lower costs while strengthening the industry's environmental sustainability. Microalgae are recognized tools for efficient wastewater treatment, offering the recycling of nutrients and water for agriculture, and producing biomass rich in growth-promoting compounds to improve plant productivity and resistance to adverse conditions. The use o...

Date: 2023   |   Origin: Repositório do LNEG

From piggery wastewater to wheat using microalgae towards zero waste

Ferreira, Alice; Figueiredo, Daniel; Ferreira, Francisca; Marujo, Ana; Bastos, Carolina R. V.; Martin-Atanes, Guillermo; Ribeiro, Belina

ABSTRACT: Microalgae production is still expensive, driving the need to lower costs while strengthening the industry's environmental sustainability. Microalgae are recognized tools for efficient wastewater treatment, offering the recycling of nutrients and water for agriculture, and producing biomass rich in growth-promoting compounds to improve plant productivity and resistance to adverse conditions. The use o...

Date: 2023   |   Origin: Repositório do LNEG

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