14 documents found, page 1 of 2
Unveiling the neurotoxicity of methylmercury in fish (Diplodus sargus) through ...
Puga, Sónia Andreia Silva; Pereira, Patrícia; Ribeiro, Filipa Pinto; O’Driscoll, Nelson J.; Mannd, Erin; Barata, Marisa; Ferreira, Pedro PousãoThe current study aims to shed light on the neurotoxicity of MeHg in fish (white seabream - Diplodus sargus) by the combined assessment of: (i) MeHg toxicokinetics in the brain, (ii) brain morphometry (volume and number of neurons plus glial cells in specific brain regions) and (iii) fish swimming behavior (endpoints associated with the motor performance and the fear/anxiety-like status). Fish were surveyed for...
Impact of an educational hands-on project on the antimicrobial, antitumor and a...
Azevedo, Maria-Manuel; Pinheiro, Céline; Dias, Alberto Carlos Pires; Ribeiro, Filipa Pinto; Baltazar, FátimaPromoting environmental and health education is crucial to allow students to make conscious decisions based on scientific criteria. The study is based on the outcomes of an Educational Project implemented with Portuguese students and consisted of several activities, exploring pre-existent Scientific Gardens at the School, aiming to investigate the antibacterial, antitumor and anti-inflammatory properties of pla...
A role of supraspinal galanin in behavioural hyperalgesia in the rat
Amorim, Diana; Pereira, Ana David; Marques, Patrícia; Puga, Sónia Andreia Silva; Rebelo, Patrícia; Costa, Patrício Soares; Pertovaara, AnttiINTRODUCTION: In chronic pain disorders, galanin (GAL) is able to either facilitate or inhibit nociception in the spinal cord but the contribution of supraspinal galanin to pain signalling is mostly unknown. The dorsomedial nucleus of the hypothalamus (DMH) is rich in galanin receptors (GALR) and is involved in behavioural hyperalgesia. In this study, we evaluated the contribution of supraspinal GAL to behaviou...
Selective impact of Tau loss on nociceptive primary afferents and pain sensation
Sotiropoulos, I.; Lopes, André T.; Pinto, Vítor; Lopes, Sofia; Carlos, Sara; Silva, Sara Carina Duarte; Carvalho, Andreia Alexandra Neves deTau protein hyperphosphorylation and consequent malfunction are hallmarks of Alzheimer's disease pathology; importantly, pain perception is diminished in these patients. In physiological conditions, Tau contributes to cytoskeletal dynamics and in this way, influences a number of cellular mechanisms including axonal trafficking, myelination and synaptic plasticity, processes that are also implicated in pain perc...
Amitriptyline reverses hyperalgesia and improves associated mood-like disorders...
Amorim, Diana; Pereira, Ana David; Pertovaara, Antti; Almeida, Armando; Ribeiro, Filipa PintoAffective disorders are common comorbidities of chronic inflammatory pain that are often overlooked in primary care. As the impact of inflammatory pain upon mood-like disorders in animal models is not well known, our objective was to assess whether prolonged experimental monoarthritis (ARTH) induced the development of anxiety and depressive-like behaviours in rodents and if amitriptyline, an antidepressant comm...
Pronociception from the dorsomedial nucleus of the hypothalamus is mediated by ...
Ribeiro, Filipa Pinto; Amorim, Diana; Pereira, Ana Carla David; Monteiro, Ana Maria; Costa, Patrício Soares; Pertovaara, Antti; Almeida, ArmandoThe dorsomedial nucleus of the hypothalamus (DMH) has been proposed to participate in stress-induced hyperalgesia through facilitation of pronociceptive cells in the rostroventromedial medulla (RVM). We hypothesized that the DMH participates in hyperalgesia induced by arthritis. The DMH was pharmacologically manipulated while assessing heat-evoked nociceptive behavior or the discharge rates of pronociceptive RV...
Pronociceptive changes in the activity of rostroventromedial medulla (RVM) pain...
Silva, M.; Amorim, Diana; Almeida, Armando; Tavares, I.; Ribeiro, Filipa Pinto; Morgado, C.Neuropathic pain is one of the most frequent complications of diabetes. The increased neuronal activity of primary afferents and spinal cord neurons in streptozotocin (STZ)-diabetic rats increases the recruitment of the nociceptive ascending pathways, which may affect the activity of pain control circuits in the brain. This study aimed to characterize the electrophysiological responses of neurons of the rostrov...
Response properties of nociceptive neurons in the caudal ventrolateral medulla ...
Ribeiro, Filipa Pinto; Ansah, Osei B.; Almeida, Armando; Pertovaara, AnttiWe characterized response properties of neurons in the caudal ventrolateral medulla (CVLM), a structure involved in pain modulation. Electrophysiological recordings were performed in pentobarbitoneanesthetized control and monoarthritic rats. Noxious pinch, heat, cold and colorectal distension were used for peripheral test stimulation. To study central modulation of CVLM neurons and role in mediating descending ...
Interaction between chronic stress and pain: the HPA axis and potential hypotha...
Ribeiro, Filipa PintoPain is an essential tool for survival, but equally important is the ability to overcome pain in life threatening events, allowing us to react. Stress-induced analgesia exemplifies this concept as a strong acute stressor activates the hypothalamic-pituitary-adrenal glands (HPA) axis and leads to the release of adrenaline, dampening pain processing and resulting in transient analgesia. This initial response is f...
Motor uncoordination and neuropathology in a transgenic mouse model of Machado-...
Fernandes, Anabela Silva; Costa, Maria do Carmo; Silva, Sara Carina Duarte; Oliveira, Pedro; Botelho, C. M.; Martins, Luís Filipe Forte OliveiraMachado-Joseph disease (MJD) is a late-onset neurodegenerative disorder caused by a polyglutamine (polyQ) expansion in the ataxin-3 protein. We generated two transgenic mouse lineages expressing the expanded human ataxin-3 under the control of the CMV promoter: CMVMJD83 and CMVMJD94, carrying Q83 and Q94 stretches, respectively. Behavioral analysis revealed that the CMVMJD94 transgenic mice developed motor unco...