5 documents found, page 1 of 1
Electrochemical immunosensor for detection of CA 15-3 biomarker in point-of-care
Rebelo, Tânia S.C.R.; Ribeiro, José A.; Sales, M. Goreti F.; Pereira, Carlos M.This work reports the development of a simple and rapid electrochemical immunosensor for the determination of breast cancer biomarker Cancer Antigen 15–3 (CA 15–3). Disposable and cost-effective chips, consisting of gold screen-printed electrodes (AuSPEs), were used to develop the portable electrochemical devices for monitoring the biomarker in point-of-care (PoC), under clinical context. The biosensor preparat...
Electrochemistry-assisted surface plasmon resonance biosensor for detection of ...
Ribeiro, José A.; Sales, Maria Goreti F.; Pereira, Carlos M.In this work, we describe an innovative methodology based on combined surface plasmon resonance (SPR) and electrochemical responses (eSPR) in the same immunoassay for screening CA 153 cancer biomarker with high sensitivity (and selectivity), in a very simple, label-free, accurate, and fully automated manner. Detection was achieved by performing two simple steps. In the first step, direct SPR was used to monitor...
Electrochemistry-assisted surface plasmon resonance detection of miRNA-145 at f...
Ribeiro, José A.; Sales, Maria Goreti Ferreira; Pereira, Carlos M.In this work, we combined electrochemical techniques with SPR (eSPR) for the label-free detection of cancer biomarker miRNA-145. Detection was performed in a simple two-step assay. In the first step, the gold sensor surface, previously functionalized with a self-assembled monolayer (SAM) of thiolated RNA probes is incubated with the sample containing the target RNA biomarker. In this step, hybridization of RNA ...
Development of hydroxybenzoic-based platforms as a solution to deliver dietary ...
Teixeira, José; Oliveira, Catarina; Amorim, Ricardo; Cagide, Fernando; Garrido, Jorge; Ribeiro, José A.; Pereira, Carlos M.; Silva, António F. M.Oxidative stress and mitochondrial dysfunction have been associated with metabolic and age-related diseases. Thus, the prevention of mitochondrial oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between the consumption of polyphenol-rich diet and the prevention of different pathologies, including diseases with a mi...
A olfactometria como método de selecção de coccinelídeos afidífagos
Garcia, Vasco; Ribeiro, José A.Um olfactómetro de difusão usado para testar duas espécies de Coccinelídeos afidífagos, Coccinella septempunctata e Adonia variegata, revelou-se eficaz. Foram oferecidas aos predadores três presas diferentes: Acyrtosiphon pisum, Megoura viciae e Aphis fabae. Os dados foram tratados estatisticamente. As conclusões mostram que existe uma preferência nítida pelas plantas infestadas com afídeos, quando comparadas c...