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Beta-carotene-loaded Oleogels: Morphological analysis, cytotoxicity assessment,...

Martins, Artur J.; Perdigão, Lara; Gonçalves, Catarina; Amado, Isabel R.; Abreu, Cristiano S.; Vicente, A. A.; Cunha, Rosiane L.; Pastrana, Lorenzo M.

Composition and structure of oleogels significantly influence their digestive behaviour, impacting triacylglycerol breakdown and the bioavailability of incorporated compounds. Texture profile analysis showed that sterol-based oleogels (STOs) exhibited 20 times higher hardness than beeswax-based oleogels (BWOs), which showed stronger cohesion due to elasticity sustained by adhesive forces. Tribological assessmen...


Use of a coaxial nozzle-assisted 3D printing and ionic crosslinking to control ...

Marques, Arlete M.; Oliveira, Sara M.; Martins, Artur J.; Bourbon, Ana Isabel Juncá Sottomayor Lisboa; Rousta, Neda; Taherzadeh, Mohammad J.

This work innovatively merges engineered food structures with additive manufacturing technology to tailor food for different dysphagia levels using filamentous Fungus Aspergillus oryzae biomass through a co-axial 3D food printing process. In order to 3D print samples with different textures, calcium chloride (CaCl2) and calcium gluconolactate (Gluco) were tested at different concentrations alongside three algin...


Alternative protein-based meat and fish analogs by conventional and novel proce...

Gürbüz, Buse N.; Pastrana, Lorenzo M.; Pereira, Ricardo Nuno Correia; Cerqueira, Miguel A.

This study aimed to explore the extent of research on developing meat and fish analogs using alternative proteins. It examined the novel and conventional technologies employed to produce these analogs and identified the primary alternative proteins that were used in their production through a systematic literature review (SLR) using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and...


Designing an antimicrobial film for wound applications incorporating bacterioph...

Pinto, Ana M.; Pereira, Raquel; Martins, Artur J.; Pastrana, Lorenzo M.; Cerqueira, Miguel A.; Sillankorva, Sanna

Alginate-based dressings have been shown to promote wound healing, leveraging the unique properties of alginate. This work aimed to develop and characterize flexible individual and bilayered films to deliver bacteriophages (phages) and -Poly-l-lysine (-PLL). Films varied in different properties. The moisture content, swelling and solubility increased with higher alginate concentrations. The water vapour permeab...


β-carotene and resveratrol loaded glycerol monostearate-based oleogels: Physico...

Barroso, Noádia G.; Martins, Artur J.; Júnior, Fernando D. O.; Okuro, Paula K.; Pereira, Rui C.; Vicente, A. A.; Pastrana, Lorenzo M.; Cunha, Rosiane L.

Oleogels are semi-solid systems that can function both as replacers of trans and saturated fats and/or as carriers of lipophilic bioactive compounds. However, bioactive compounds can affect the structure of the oleogel matrix and this effect depends on the properties of such compounds. Therefore, the aim of this study was to develop oleogels loaded with -carotene (BC) or resveratrol (R), with low concentrations...


Strategies for the incorporation of organosolv lignin in hydroxypropyl methylce...

Silva, Lúcio; Colussi, F.; Martins, Joana T.; Vieira, Jorge Miguel Magalhães; Pastrana, Lorenzo M.; Teixeira, J. A.; Cerqueira, Miguel A.

Organosolv lignin extracted from vine pruning residues was added to hydroxypropyl methylcellulose (HPMC)-based films using three strategies: i) lignin incorporated into the film (lignin-based film), ii) lignin nanoparticles (LNPs) incorporated into the film (LNPs-based film), and iii) lignin coated on HPMC films' surface (lignin-coated film). The films obtained were evaluated in terms of morphology, water barri...


Active Low-Density Polyethylene-Based Films by Incorporating α-Tocopherol in th...

Azevedo, Ana G.; Barros, Carolina; Miranda, Sónia; Machado, A. V.; Carneiro, O. S.; Silva, Bruno; Andrade, Mariana A.; Vilarinho, Fernanda

In this work, alpha-tocopherol (α-TOC) was encapsulated in poly(lactic acid) nanoparticles (PLA NPs) and added to low-density polyethylene (LDPE) films with the aim of producing an active film for food packaging applications. PLA NPs loaded with α-TOC were produced through nanoprecipitation and dried using two methods (freeze-dryer and oven). LDPE-based films with final polymeric matrix concentrations of 10 and...


Multilayer film comprising polybutylene adipate terephthalate and cellulose nan...

Melendez-Rodriguez, Beatriz; Prieto, Cristina; Pardo-Figuerez, Maria; Angulo, Inmaculada; Bourbon, Ana I.; Amado, Isabel R.

In the present study, a multilayer, high-barrier, thin blown film based on a polybutylene adipate terephthalate (PBAT) blend with polyhydroxyalkanoate (PHA), and composed of four layers including a cellulose nanocrystal (CNC) barrier layer and an electrospun poly(3-hydroxybutyrateco-3-hydroxyvalerate) (PHBV) hot-tack layer, was characterized in terms of the surface roughness, surface tension, migration, mechani...


Edible alginate-based films with anti-SARS-CoV-2 activity

Cerqueira, Miguel A.; Leite, Ana C. C. O.; Tomás, Ana L.; Reichel, Anna; Silva, Patrícia M.; Santos, Nuno C.; Michelin, Michele; Fuciños, Pablo

The viability of SARS-CoV-2 on food surfaces and its propagation through the food chain has been discussed by several stakeholders, as it may represent a serious public health problem, bringing new challenges to the food system. This work shows for the first time that edible films can be used against SARS-CoV-2. Sodium alginate-based films containing gallic acid, geraniol, and green tea extract were evaluated i...


Bacteriophage delivery systems for food applications: Opportunities and perspec...

Costa, Maria J.; Pastrana, Lorenzo M.; Teixeira, J. A.; Sillankorva, Sanna; Cerqueira, Miguel Ângelo Parente Ribeiro

Currently, one-third of all food produced worldwide is wasted or lost, and bacterial contamination is one of the main reasons. Moreover, foodborne diseases are a severe problem, causing more than 420,000 deaths and nearly 600 million illnesses yearly, demanding more attention to food safety. Thus, new solutions need to be explored to tackle these problems. A possible solution for bacterial contamination is usin...


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