22 documents found, page 1 of 3
Taurine supplementation to Plant-Based Diets improves lipid metabolism in Seneg...
Raquel Cêa de Aragão Teixeira, Cláudia; Teodósio, Rita; Colen, R.; Richard, Nadège; Rønnestad, Ivar; Dias, Jorge; Conceição, Luís E. C.; Ribeiro, LauraTaurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal diets on the metabolism of taurine, bile acids, and lipids of Senegalese sole. A fishmeal (FM) and a plant-protein-based (PP0) di...
Supplementing taurine to plant-based diets improves lipid digestive capacity an...
Richard, Nadège; Colen, R.; Aragão, CláudiaRecent studies reported positive effects of taurine supplementation to plant protein-based diets on fish growth performance and feed conversion ratio but the mechanisms involved remain unclear. The objective of this work was to provide a first insight on the importance of taurine on dietary protein and lipid absorption and utilisation, in Senegalese sole (Solea senegalensis) juveniles fed plant-based diets. Sev...
Visualization and Differential Analysis of Protein Expression Data Using R.
Silva, Tomé; Richard, NadègeData analysis is essential to derive meaningful conclusions from proteomic data. This chapter describes ways of performing common data visualization and differential analysis tasks on gel-based proteomic datasets using a freely available statistical software package (R). A workflow followed is illustrated using a synthetic dataset as example.
Nutritional mitigation of winter thermal stress in gilthead seabream: associate...
Richard, Nadège; Silva, Tome S.; Wulff, Tune; Schrama, Denise; Dias, Jorge P.; Rodrigues, Pedro; Conceicao, LuisA trial was carried out with gilthead seabream juveniles, aiming to investigate the ability of an enhanced dietary formulation (diet Winter Feed, WF, containing a higher proportion of marine-derived protein sources and supplemented in phospholipids, vitamin C, vitamin E and taurine) to assist fish in coping with winter thermal stress, compared to a low-cost commercial diet (diet CTRL). In order to identify the ...
Enhanced dietary formulation to mitigate winter thermal stress in gilthead sea ...
Schrama, Denise; Richard, Nadège; Silva, Tomé S.; Figueiredo, Filipe A.; Conceição, Luís E. C.; Burchmore, Richard; Eckersall, David; Rodrigues, PedroLow water temperatures during winter are common in farming of gilthead sea bream in the Mediterranean. This causes metabolic disorders that in extreme cases can lead to a syndrome called "winter disease." An improved immunostimulatory nutritional status might mitigate the effects of this thermal metabolic stress. A trial was set up to assess the effects of two different diets on gilthead sea bream physiology an...
Dietary indispensable amino acids profile affects protein utilization and growt...
Canada, Paula; Engrola, S.; Richard, Nadège; Lopes, Ana Filipa; Pinto, Wilson; Valente, Luísa M. P.; Da Conceição, Gisele CristinaIn diet formulation for fish, it is critical to assure that all the indispensable amino acids (IAA) are available in the right quantities and ratios. This will allow minimizing dietary AA imbalances that will result in unavoidable AA losses for energy dissipation rather than for protein synthesis and growth. The supplementation with crystalline amino acids (CAA) is a possible solution to correct the dietary ami...
Metabolic fingerprinting of gilthead seabream (Sparus aurata) liver to track in...
Silva, Tomé S.; Costa, Ana M. Rosa da; Conceição, L. E. C.; Dias, Jorge P.; Rodrigues, Pedro; Richard, NadègeFarmed gilthead seabream is sometimes affected by a metabolic syndrome, known as the "winter disease", which has a significant economic impact in the Mediterranean region. It is caused, among other factors, by the thermal variations that occur during colder months and there are signs that an improved nutritional status can mitigate the effects of this thermal stress. For this reason, a trial was undertaken wher...
Two-dimensional proteomics as a tool to evaluate nutritional effects in farmed ...
Richard, Nadège; Gavaia, Paulo J.; De Vareilles, M.; Silva, Tomé S.; Cordeiro, O.; Rodrigues, Pedro; Conceição, L. E. C.The use of proteomics in fish research is at a relatively early stage compared to terrestrial vertebrates. It has already been applied in the field of fish nutrition, enabling to point out metabolic changes occurring in response to dietary manipulations such as a variation in energy content, dietary nitrogen compostion, lipid composition or the incorporation of plant protein sources in aquafeeds.
Impact of dietary protein hydrolysates on skeleton quality and proteome in Dipl...
Vareilles, Mahaut; Richard, Nadège; Gavaia, Paulo J.; Silva, Tomé S.; Cordeiro, O.; Guerreiro, Inês; Yúfera, M.; Batista, I.; Pires, C.In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co-fed from first-feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP-90; H – inclusion of a high amount in 0.5–30...
Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a ...
Vareilles, Mahaut; Conceição, L. E. C.; Gómez-Requeni, Pedro; Kousoulaki, Katerina; Richard, Nadège; Rodrigues, Pedro; Fladmark, K. E.; Rønnestad, I.Lysine (Lys) is an indispensable amino acid (AA) and generally the first limiting AA in vegetable protein sources in fish feeds. Inadequate dietary Lys availability may limit protein synthesis, accretion and growth of fish. This experiment aimed to further elucidate the role of Lys imbalance on growth by examining the myotomal muscle proteome of juvenile zebrafish (Danio rerio). Quadruplicate groups of 8 fish w...