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Preliminary results concerning school staff personal exposure to microbial load...

Pena, Pedro; Cervantes, Renata; Dias, Marta; Gomes, Bianca; Riesenberger, Bruna; Marques, Liliana; Rodriguez, Margarida; Viegas, Carla

School environments are potential exposure scenarios to bioaerosols. Breading such contaminants can contribute to the development of respiratory infection. Most studies performed in schools regarding air quality focus on children’s health, neglecting the personal exposure of teachers and school staff. This study aims to provide a first insight into the personal exposure of teachers and school staff to bacteria ...


Levels of fungi in the air of poultry farms following different stages of birds...

Gomes, Bianca; Dias, Marta; Pena, Pedro; Cervantes, Renata; Rodriguez, Margarida; Marques, Liliana; Riesenberger, Bruna; Carolino, Elisabete

Poultry production intensification might influence microbial development. Concerning fungal assessment, despite the limited number of studies, several fungi have already been found in poultry pavilions. Occupational health specialists frequently overlook worker safety and biological risk assessment due to a lack of systematic knowledge regarding the agents involved. Consequently, workers, exposed to organic dus...


Sampling protocol to assess Aspergillus section fumigati in woodworking environ...

Dias, Marta; Gomes, Bianca; Pena, Pedro; Cervantes, Renata; Rodriguez, Margarida; Riesenberger, Bruna; Marques, Liliana; Ribeiro, Edna; Viegas, Carla

Azole fungicides are commonly used in sawmills to protect wood against the wood-destroying basidiomycete fungi. Azole-resistant Aspergillus section Fumigati has previously been identified in these environments. Here we aimed to establish a sampling protocol to efficiently isolate Aspergillus section Fumigati in woodworking environments. The sampling campaign was conducted between December 2022 and November 2023...


Boosting knowledge on occupational exposure to microbial contamination in Portu...

Dias, Marta; Gomes, Bianca; Pena, Pedro; Cervantes, Renata; Rodriguez, Margarida; Riesenberger, Bruna; Marques, Liliana; Carolino, Elisabete

Introduction: Wood industry workers face health risks due to exposure to microorganisms and their metabolites. This study aimed to characterize seasonal microbial contamination, antifungal resistance, mycotoxins, cytotoxicity, and particulate matter in Portuguese carpentries, to reduce exposure and promote safe working conditions. Methods: Conducted in six carpentries in Lisbon, Portugal, the sampling strategy ...


Critical insights on fungal contamination in schools: a comprehensive review of...

Cervantes, Renata; Pena, Pedro; Riesenberger, Bruna; Rodriguez, Margarida; Henderson, Drew; Gonçalves, Sara; Newire, Enas; Pogner, Clara; Salonen, Heidi

This review addresses the increasing problem of fungal contamination in schools, which has a profound impact on indoor air quality and student health. Fungal contamination creates health problems such as respiratory problems, allergies, which can be particularly harmful in schools (e.g., Aspergillus fumigatus and Fusarium sp. are especially important as they are well-known indoor allergens and can induce seriou...


Fungal contamination in Lisbon’s primary schools: sampling insights and analyti...

Cervantes, Renata; Pena, Pedro; Gomes, Bianca; Dias, Marta; Riesenberger, Bruna; Rodriguez, Margarida; Marques, Liliana; Viegas, Carla

Climate change is posing challenges for Portugal due to intense weather changes, affecting public health and causing pathogens to adapt and spread, increasing the global risk of infectious diseases. Azole fungicides are less effective against resistant fungi, raising concerns for children. Warm and humid conditions promote the growth of pathogenic fungi and the production of mycotoxins, impacting health by caus...


Budget-friendly protocol for TR34/L98H and TR46/Y121F/T289A mutation detection ...

Dias, Marta; Rodriguez, Margarida; Vasques, C.; Riesenberger, Bruna; Marques, Liliana; Gomes, Bianca; Pena, Pedro; Cervantes, Renata; Viegas, Susana

Aspergillus section Fumigati is one of the most common sections, in the environment. It has been found in different occupational environments, such as sawmills and waste sorting. Its cryptic species show intrinsic resistance to several antifungals. Resistance in A. fumigatus is emerging due to selective pressure caused by the prolonged use of azoles. It is often associated with mutations in the Cyp51A gene. The...


Sampling protocol to assess Aspergillus section Fumigati in woodworking environ...

Dias, Marta; Gomes, Bianca; Pena, Pedro; Cervantes, Renata; Rodriguez, Margarida; Riesenberger, Bruna; Marques, Liliana; Ribeiro, Edna; Viegas, Carla

Portugal’s wood industry employs over 80,000 people and comprises about 16,600 enterprises in 2021. Sawmill workers are exposed to several occupational hazards, including microorganisms. Health problems have been documented by workers exposed to wood dust infected with fungi. A fungal priority pathogens list (WHO FPPL) was recently released by the WHO. It prioritizes fungal pathogens based on their perceived pu...


Filling the knowledge gap regarding microbial occupational exposure assessment ...

Riesenberger, Bruna; Rodriguez, Margarida; Marques, Liliana; Cervantes, Renata; Gomes, Bianca; Dias, Marta; Pena, Pedro; Ribeiro, Edna; Viegas, Carla

Background: Wastewater treatment plants (WWTPs) are crucial in the scope of European Commission circular economy implementation. However, bioaerosol production may be a hazard to occupational and public health. A scoping review regarding microbial contamination exposure assessment in WWTPs was performed. Methods: This study was performed through PRISMA methodology in PubMed, Scopus, and Web of Science. Results:...


Preliminary results concerning school staff personal exposure to microbial load...

Pena, Pedro; Cervantes, Renata; Dias, Marta; Gomes, Bianca; Riesenberger, Bruna; Marques, Liliana; Rodriguez, Margarida; Viegas, Carla

Why is it important to assess indoor air quality in schools? Is Portugal addressing educators’ health risks? Do the current guidelines cover school workers too? This study aims to provide a first insight into the personal exposure of teachers and school staff to bacteria and fungi in primary schools in Portugal.


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