6 documents found, page 1 of 1
Partial coated stem cells with bioinspired silica as new generation of cellular...
Maciel, Marta M.; Correia, Tiago R.; Gaspar, Vítor M.; Rodrigues, João M. M.; Choi, Insung S.; Mano, J. F.The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica ...
Partial coated stem cells with bioinspired silica as new generation of cellular...
Maciel, Marta M.; Correia, Tiago R.; Gaspar, Vítor M.; Rodrigues, João M. M.; Choi, Insung S.; Mano, João F.The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica ...
Enzymatically degradable, starch-based layer-by-layer films: application to cyt...
Moon, Hee Chul; Han, Sol; Borges, João; Pesqueira, Tamagno; Choi, Hyunwoo; Han, Sang Yeong; Cho, Hyeoncheol; Park, Ji Hun; Mano, João F.The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/AL...
Geometrically controlled liquefied capsules for modular tissue engineering stra...
Nadine, Sara; Patrício, Sónia G.; Barrias, Cristina C.; Choi, Insung S.; Matsusaki, Michiya; Correia, Clara R.; Mano, João F.A plethora of bioinspired cell-laden hydrogels are being explored as building blocks that once assembled are able to create complex and highly hierarchical structures recapitulating the heterogeneity of living tissues. Yet, the resulting 3D bioengineered systems still present key limitations, mainly related with limited diffusion of essential molecules for cell survival, which dictates the failure of most strat...
Nanogrooved microdiscs for bottom-up modulation of osteogenic differentiation
Bjorge, Isabel M.; Choi, Insung S.; Correia, Clara R.; Mano, João F.Grooved topographical features have effectively modulated cell differentiation on two-dimensional substrates. To transpose patterning into a 3D environmment, nanogrooved microdiscs, "topodiscs", are produced as cell carriers for bottom-up cell-mediated assembly. While enhancing cell proliferation, topodiscs led to the formation of bone-like aggregates, even in culture medium lacking osteoinductive factors.
Bioinspired ultratough hydrogel with fast recovery, self-healing, injectability...
Azevedo, Sara; Costa, Ana M. S.; Andersen, Amanda; Choi, Insung S.; Birkedal, Henrik; Mano, João F.Inspired by the mussel byssus adhesiveness, a highly hydrated polymeric structure is designed to combine, for the first time, a set of interesting features for load-bearing purposes. These characteristics include: i) a compressive strength and stiffness in the MPa range, ii) toughness and the ability to recover it upon successive cyclic loading, iii) the ability to quickly self-heal upon rupture, iv) the possib...