3 documents found, page 1 of 1
Inter-laboratory variation in measurement of DNA damage by the alkaline comet a...
Moller, P; Azqueta, A; Collia, M; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, S; Scavone, FThe comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of DNA damage. Certain steps in the comet assay procedure have substantial impact on the magnitude of DNA migration (e.g. electric potential and time of electrophoresis). Inter-l...
DNA strand break levels in cryopreserved mononuclear blood cell lines measured ...
Moller, P; Azqueta, A; Rodriguez-Garraus, A; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, SThe comet assay is widely used in biomonitoring studies for the analysis of DNA damage in leukocytes and peripheral blood mononuclear cells. Rather than processing blood samples directly, it can be desirable to cryopreserve whole blood or isolated cells for later analysis by the comet assay. However, this creates concern about artificial accumulation of DNA damage during cryopreservation. In this study, 10 labo...
Long-term cryopreservation of potassium bromate positive assay controls for mea...
Moller, P; Azqueta, A; Rodriguez-Garraus, A; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, SThe formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay is widely used for the measurement of oxidatively generated damage to DNA. However, there has not been a recommended long-term positive control for this version of the comet assay. We have investigated potassium bromate as a positive control for the Fpg-modified comet assay because it generates many Fpg-sensitive sites with a little concurrent g...