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Detection of hepatitis E virus genotype 3 in an Algerian mouse (Mus spretus) in...

Santos-Silva, S; Moraes, DFDSD; López-López, P; Paupério, J; Queirós, J; Rivero-Juarez, A; Lux, L; Ulrich, RG; Gonçalves, HMR; Van der Poel, WHM

Virus monitoring in small mammals is central to the design of epidemiological control strategies for rodent-borne zoonotic viruses. Synanthropic small mammals are versatile and may be potential carriers of several microbial agents. In the present work, a total of 330 fecal samples of small mammals were collected at two sites in the North of Portugal and screened for zoonotic hepatitis E virus (HEV, species Pasl...


Multicentric Study on Enteric Protists Occurrence in Zoological Parks in Portugal

Mega, J; Moreira, R; Moreira, G; Silva-Loureiro, A; Gomes da Silva, P; Istrate, C; Santos-Silva, S; Rivero-Juarez, A; Carmena, D; Mesquita, JR

Parasitic infections of the gastrointestinal tract of domestic animals play a major role in the transmission of disease, which in turn may result in financial and productive losses. Notwithstanding, studies on the burden and distribution of diarrheagenic protists in zoological gardens are still insufficient. Given the close animal-animal and animal-human interaction in these settings, Public Health concerns und...


Hepatitis E virus in wild and domestic rabbits from Portugal: a combined molecu...

Santos-Silva, S; Santos, N; López-López, P; Nascimento, MSJ; Gonçalves, HMR; Van der Poel, WHM; Rivero-Juarez, A; Mesquita, JR

Hepatitis E virus (HEV), species Paslahepevirus balayani, poses a global public health threat, especially in developing countries, by causing acute enterically transmitted hepatitis. HEV infects various mammalian hosts and belongs to the genus Paslahepevirus in the family Hepeviridae. While swine are recognized as the main hosts of HEV, rabbits, which can also be affected by swine HEV-3 related strains, serve a...


Serological and Molecular Survey of Hepatitis E Virus in Small Ruminants from C...

Santos-Silva, S; Romalde, JL; Bento, JT; Cruz, AVS; López-López, P; Gonçalves, HMR; Van der Poel, WHM; Nascimento, MSJ; Rivero-Juarez, A; Mesquita, JR

Hepatitis E virus (HEV) is currently recognized as an emerging problem and a growing concern for public health in developed countries, with HEV infections mainly attributable to foodborne transmission of HEV-3. The zoonotic HEV genotype 3 infects a wide range of mammalian hosts, with swine considered as the primary host. This study investigates the occurrence of HEV among small ruminants in Portugal. The primar...


A Systematic Review and Meta-Analysis on Hepatitis E Virus Detection in Farmed ...

Santos-Silva, S; López-López, P; Gonçalves, HMR; Rivero-Juarez, A; Van der Poel, WHM; Nascimento, MSJ; Mesquita, JR

Swine are widely recognized as the main reservoir of zoonotic HEV; however, a growing body of data on the HEV prevalence in farmed ruminants of different species also points to a potential route for HEV transmission through ruminants and ruminant products and by-products. Definite information on the zoonotic potential of ruminants is still absent or unclear, compelling the necessity for increasing knowledge on ...


Optimization of the molecular diagnosis of the acute hepatitis E virus infection

Lopez-Lopez, P; Frias, M; Perez-Jimenez, AB; Freyre-Carrillo, C; Pineda, JA; Aguilera, A; Fuentes, A; Alados, JC; Reina, G; Ramirez-Arellano, E

To evaluate the diagnostic value of the combination of two broad-range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV-IgM antibodies were tested together with evaluation of HEV viraemia by two PCR assays (ORF3 and ORF1). The number of individuals exh...


Hepatitis E Virus in the Iberian Peninsula: A Systematic Review

Santos-Silva, S; Moraes, DFDD; López-López, P; Rivero-Juarez, A; Mesquita, JR; Nascimento, MSJ

One of the most frequent causes of acute viral hepatitis is hepatitis E virus (HEV) causing 20 million infections worldwide each year and 44,000 deaths. Studies on HEV in the Iberian Peninsula have been increasing through time with HEV infection being identified in humans and animals. The aim of the present systematic review was to compile and evaluate all the published data on HEV from studies performed in hum...


Survey of Zoonotic Diarrheagenic Protist and Hepatitis E Virus in Wild Boar (Su...

Santos-Silva, S; Moraes, DFDD; Lopez-Lopez, P; Palmeira, JD; Torres, RT; Nascimento, MS; Dashti, A; Carmena, D; Rivero-Juarez, A; Mesquita, JR

Simple Summary Enteropathogenic viruses, such as hepatitis E virus, and diarrhoeagenic protists have been frequently reported in swine and can infect a wide range of mammals, including humans. Data on their fecal shedding and circulation pathways are still lacking or incomplete. Hence, the aim of the present study was to characterize the presence of microeukaryotes and HEV in the wild boar of Portugal. Of the 1...


A Systematic Review of Hepatitis E Virus Detection in Camels

Santos-Silva, S; Hemnani, M; Lopez-Lopez, P; Gonçalves, HMR; Rivero-Juarez, A; van der Poel, WHM; Nascimento, MSJ; Mesquita, JR

Simple Summary: Acute hepatitis, which is a rising public health issue globally, is mostly caused by the hepatitis E virus (HEV). There is a potential risk of camel-borne zoonotic HEV infection in the desert regions of the Middle East and Africa, where camels frequently interact with human populations and camel-derived food products constitute a component of the food chain. To better understand the current stat...


Balantioides coli Fecal Excretion in Hunted Wild Cervids (Cervus elaphus and Da...

Mega, J; Santos-Silva, S; Loureiro, A; Palmeira, JD; Torres, RT; Rivero-Juarez, A; Carmena, D; Mesquita, J

Balantioides coli is a zoonotic enteric protozoan parasite of public veterinary health relevance and a concern in animal production and food safety. While wild cervids are recognized reservoirs for several zoonotic pathogens, little is known about the occurrence of B. coli in deer species, especially in Europe. To fill this gap, a total of 130 fecal samples from legally hunted red deer (Cervus elaphus, n = 95) ...


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