30 documents found, page 1 of 3
Immunization with the HisAK70 DNA vaccine induces resistance against Leishmania...
Rodrigo, Abel Martínez; Dias, Daniel Silva; Ribeiro, Patrícia Aparecida Fernandes; Roatt, Bruno Mendes; Zubiri, Alicia MasLeishmania amazonensis is the aetiological agent of a broad spectrum of leishmaniosis in South America. It can cause not only numerous cases of cutaneous leishmaniosis but also diffuse cutaneous leishmaniosis. Considering the diversity of parasite species causing different forms of the disease that coexist in the same region, it is desirable to develop a vaccine capable of eliciting cross-protection. We have pr...
Synthetic peptides elicit strong cellular immunity in Visceral Leishmaniasis na...
Brito, Rory Cristiane Fortes de; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Mathias, Fernando Augusto SiqueiraReverse vaccinology or immunoinformatics is a computational methodology which integrates data from in silico epitope prediction, associated to other important information as, for example, the predicted subcellular location of the proteins used in the design of the context of vaccine development. This approach has the potential to search for new targets for vaccine development in the predicted proteome of pathog...
Neutrophil properties in healthy and Leishmania infantum-naturally infected dogs.
Wardini, Amanda Brito; Silva, Lucia Helena Pinto da; Nadaes, Natalia Rocha; Nascimento, Michelle Tanny Cunha do; Roatt, Bruno MendesVisceral leishmaniasis is a chronic disease that afects humans and dogs as well. Dogs, the domestic reservoir of Leishmania, play a central role in the transmission of visceral leishmaniasis, the most severe form of this disease. Neutrophils are the most abundant leukocytes in blood and interact with the parasite after infection. Here, we evaluate the efector properties of neutrophils from healthy and naturally...
In vivo antileishmanial efficacy of a naphthoquinone derivate incorporated into...
Mendonça, Débora Vasconcelos Costa; Tavares, Grasiele de Sousa Vieira; Lage, Daniela Pagliara; Soyer, Tauane Gonçalves; Carvalho, Lívia MendesNew therapeutic strategies against leishmaniasis are desirable, since the treatment against disease presents problems, such as the toxicity, high cost and/or parasite resistance. As consequence, new antileishmanial compounds are necessary to be identified, as presenting high activity against Leishmania parasites, but low toxicity in mammalian hosts. Flau-A is a naphthoquinone derivative recently showed to prese...
Peptide vaccines for leishmaniasis.
Brito, Rory Cristiane Fortes de; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Vieira, João Filipe PereiraDue to an increase in the incidence of leishmaniases worldwide, the development of new strategies such as prophylactic vaccines to prevent infection and decrease the disease have become a high priority. Classic vaccines against leishmaniases were based on live or attenuated parasites or their subunits. Nevertheless, the use of whole parasite or their subunits for vaccine production has numerous disadvantages. T...
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features...
Resende, Lucilene Aparecida; Soares, Rodrigo Dian de Oliveira Aguiar; Ker, Henrique Gama; Roatt, Bruno Mendes; Mendonça, Ludmila Zanandreis deFundação de Amparo a Pesquisa do Estado de Minas Gerais. Belo Horizonte, MG, Brazil; Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil/Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas/NUPEB Laboratório de Imunopatologia. Ouro Preto, MG, Brazil; Uni...
Poloxamer 407 (Pluronic® F127)-based polymeric micelles for amphotericin B : in...
Mendonça, Débora Vasconcelos Costa; Lage, Letícia Martins dos Reis; Lage, Daniela Pagliara; Chávez Fumagalli, Miguel Angel; Ludolf, FernandaIn the present study, a Poloxamer 407-based amphotericin B (AmpB)-containing polymeric micelles system (AmpB/M) was employed in the treatment of Leishmania amazonensis-infected BALB/c mice. Initially, the in vitro antileishmanial activity (IC50 value) of AmpB/M and B-AmpB/M (empty micelles) against stationary promastigotes and amastigotes-like forms of the parasites was determined, and results were of 1.83 ± 0....
LBSapSal-vaccinated dogs exhibit increased circulating T-lymphocyte subsets (CD...
Soares, Rodrigo Dian de Oliveira Aguiar; Roatt, Bruno Mendes; Ker, Henrique Gama; Moreira, Nádia das Dores; Mathias, Fernando Augusto SiqueiraBackground: The development of a protective vaccine against canine visceral leishmaniasis (CVL) is an alternative approach for interrupting the domestic cycle of Leishmania infantum. Given the importance of sand fly salivary proteins as potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in the last few deca...
A vaccine therapy for canine visceral leishmaniasis promoted significant improv...
Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Levi Eduardo Soares; Cardoso, Jamille Mirelle de OliveiraHerein, we evaluated the treatment strategy employing a therapeutic heterologous vaccine composed of antigens of Leishmania braziliensis associated with MPL adjuvant (LBMPL vaccine) for visceral leishmaniasis (VL) in symptomatic dogs naturally infected by Leishmania infantum. Sixteen dogs received immunotherapy with MPL adjuvant (n = 6) or with a vaccine composed of antigens of L. braziliensis associated with M...
Multicomponent LBSap vaccine displays immunological and parasitological profile...
Mendonça, Ludmila Zanandreis de; Resende, Lucilene Aparecida; Lanna, Mariana Ferreira; Soares, Rodrigo Dian de Oliveira Aguiar; Roatt, Bruno MendesBackground: In past years, many researchers have sought canine visceral leishmaniasis (CVL) prevention through the characterization of Leishmania antigens as vaccine candidates. Despite these efforts, there is still no efficient vaccine for CVL control. Methods: In the present study, we performed a pre-clinical vaccine trial using BALB/c mice to compare the effects of the multicomponent LBSap vaccine with those...