6 documents found, page 1 of 1
Expression of the sucrose binding protein from soybean: renaturation and stabil...
Rocha, Carolina S.; Luz, Dirce F.; Oliveira, Marli L.; Baracat-Pereira, Maria C.; Medrano, Francisco Javier; Fontes, Elizabeth P.B.The sucrose binding protein (SBP) belongs to the cupin family of proteins and is structurally related to vicilin-like storage proteins. In this investigation, a SBP isoform (GmSBP2/S64) was expressed in E. coli and large amounts of the protein accumulated in the insoluble fraction as inclusion bodies. The renatured protein was studied by circular dichroism (CD), intrinsic fluorescence, and binding of the hydrop...
Combinatorial regulation modules on GmSBP2 promoter: a distal cis-regulatory do...
Waclawovsky, Alessandro J.; Freitas, Rejane L.; Rocha, Carolina S.; Contim, Luis Antônio S.; Fontes, Elizabeth P.B.The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of reporter genes in transgenic tobacco. Here, we identified cis-regulatory domains (CRD) that contribute with positive and negative regulation for the tissue-specific pattern of the GmSPB2 promoter. Negative regulatory elements in the distal CRD-A (−2000 to −700) sequences suppressed expression from the GmSBP2 promoter...
Synonymous site variation due to recombination explains higher genetic variabil...
Lima, Alison T. M.; Sobrinho, Roberto R.; González-Aguilera, Jorge; Rocha, Carolina S.; Silva, Sarah J. C.; Xavier, César A. D.; Silva, Fábio N.Begomoviruses are ssDNA plant viruses that cause serious epidemics in economically important crops worldwide. Non-cultivated plants also harbour many begomoviruses, and it is believed that these hosts may act as reservoirs and as mixing vessels where recombination may occur. Begomoviruses are notoriously recombination-prone, and also display nucleotide substitution rates equivalent to those of RNA viruses. In B...
The ribosomal protein L10/QM-like protein is a component of the NIK-mediated an...
Rocha, Carolina S.; Santos, Anésia A.; Machado, João Paulo B.; Fontes, Elizabeth P.B.The NIK (NSP-interacting kinase)-mediated antiviral signaling pathway was identified as a virulence target of the begomovirus nuclear shuttle protein (NSP). Here, we further characterized this layer of plant innate defense by identifying the ribosomal protein L10 (rpL10), a QM-like protein, as a downstream effector of the antiviral signaling. Although both ribosomal proteins rpL10 and rpL18 were found to associ...
Brazilian begomovirus populations are highly recombinant, rapidly evolving, and...
Rocha, Carolina S.; Castillo-Urquiza, Gloria P.; Lima, Alison T. M.; Silva, Fábio N.; Xavier, Cesar A. D.; Hora-Júnior, Braz T.The incidence of begomovirus infections in crop plants sharply increased in Brazil during the 1990s following the introduction of the invasive B biotype of the whitefly vector, Bemisia tabaci. It is believed that this biotype transmitted begomoviruses from noncultivated plants to crop species with greater efficiency than indigenous B. tabaci biotypes. Either through rapid host adap- tation or selection pressure...
Regulated nuclear trafficking of rpL10A mediated by NIK1 represents a defense s...
Carvalho, Claudine M.; Santos, Anésia A.; Pires, Silvana R.; Rocha, Carolina S.; Saraiva, Daniela I.; Machado, João Paulo B.; Mattos, Eliciane C.The NSP-interacting kinase (NIK) receptor-mediated defense pathway has been identified recently as a virulence target of the geminivirus nuclear shuttle protein (NSP). However, the NIK1–NSP interaction does not fit into the elicitor–receptor model of resistance, and hence the molecular mechanism that links this antiviral response to receptor activation remains obscure. Here, we identified a ribosomal protein, r...