O principal desafio que enfrenta este projeto é o envelhecimento da população, uma questão desafiante para as políticas dos países, Espanha e Portugal, e que se estende com maior incidência nas regiões que cooperam no projeto (EuroACE: Extremadura, Centro de Portugal e Alentejo) e que têm características comuns: reduzida densidade populacional e um acentuado envelhecimento. Este projeto enfrenta assim desafios ...
Geographic location and, particularly, soil and climate exert influence on the typicality of a wine from a specific region, which is often justified by the terroir, and these factors also influence the epiphytic flora associated with the surface of the grape berries. In the present study, the microbiome associated with the surface of berries obtained from ten vineyards of the Alvarinho variety located in differ...
Serra da Estrela is the oldest and most recognizable traditional protected designation of origin (PDO) cheese from Portugal. It has been extensively studied over the years, but the latest microbial characterization is 20 years old. Hence, this work aimed to perform an updated characterization of Serra da Estrela PDO cheeses and raw materials. Our analysis showed that lactic acid bacteria content on Serra da Est...
Salmonella is a Gram-negative flagellated rod-shaped bacterium that is one of the most important etiological agents in bacterial foodborne diseases [1,2]. Despite human salmonellosis generally presenting as a self-limiting episode of enterocolitis, the infection can degenerate into chronic and debilitating conditions [2]. To diagnose a Salmonella infection, standard cultural methods are routinely used, which im...
The prognosis of the human immunodeficiency virus (HIV) infected patients has significantly improved over the last decades. On the one hand, with the development of antiretroviral therapy (ART), Kaposi sarcoma (KS) has become a rare complication. On the other hand, with the increase in life expectancy of this population, an increased risk of cardiovascular diseases, such as acute myocardial infarction (AMI), pe...
Flow-Fluorescence in situ hybridization (Flow-FISH) enables multiparametric high-throughput detection of target nucleic acid sequences at the single cell-level, allowing an accurate quantification of different cell populations by using a combination of flow cytometry and fluorescent in situ hybridization (FISH). In this chapter, a flow-FISH protocol is described with labeled nucleic acid mimics (NAMs) (e.g. LNA...
Foodborne diseases are a major global public health concern. The gold standard detection techniques, namely culture plating techniques, are nowadays considered inadequate for the modern food industry mainly due to the time requirements of this sector. As such, the adoption of faster detection methods to be routinely used in screening the protocols of foodborne pathogens is required. Fluorescence in situ Hybridi...
A universidade medieval, enquanto instituição cultural por excelência e veículo essencial da produção e desenvolvimento intelectual da Europa de então, era constituída por corpos académicos, entre os quais o oficialato do Estudo, responsável pela administração e burocracia universitária. Nesse sentido, propomos tratar de um dos mais importantes oficiais da universidade medieval: o bedel. Neste artigo, com base ...