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Unveiling biological activities of marine fungi: the effect of sea salt
Gonçalves, Micael F. M.; Paço, Ana; Escada, Luís F.; Albuquerque, Manuela S. F.; Pinto, Carlos A.; Saraiva, Jorge A.; Duarte, Ana SofiaThere is an urgent need for new substances to overcome current challenges in the health sciences. Marine fungi are known producers of numerous compounds, but the manipulation of growth conditions for optimal compound production can be laborious and time-consuming. In Portugal, despite its very long coastline, there are only a few studies on marine fungi. From a collection of Portuguese marine fungi, we screened...
Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Struct...
Rodrigues, Dina; Costa-Pinto, Ana R.; Sousa, Sérgio; Vasconcelos, Marta W.; Pintado, Manuela M.; Pereira, Leonel; Rocha-Santos, Teresa A. P.Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in ...
Sargassum muticum and Osmundea pinnatifida enzymatic extracts: chemical, struct...
Rodrigues, Dina; Costa-Pinto, Ana R.; Sousa, Sérgio; Vasconcelos, Marta W.; Pintado, Manuela M.; Pereira, Leonel; Rocha-Santos, Teresa A. P.Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in ...
Chemical and structural characterization of Pholiota nameko extracts with biolo...
Rodrigues, Dina; Freitas, Ana C.; Sousa, Sérgio; Amorim, Manuela; Vasconcelos, Marta W.; Costa, João P. da; Silva, Artur M. S.Edible mushrooms including Pholiota nameko are excellent sources of extractable bioactive compounds with much to explore. Enzymatic extractions with Cellulase and Viscozyme were responsible for highest extraction yields (67-77%). No strong antioxidant activity was observed although extracts were able to scavenge ABTS(+) and OH(+). Potential prebiotic activity was observed in all extracts, some increasing 1.4-2 ...
Graphene based sensors and biosensors
Justino, Celine I. L.; Gomes, Ana R.; Freitas, Ana C.; Duarte, Armando C.; Rocha-Santos, Teresa A. P.Graphene has contributed to the fabrication of sensitive sensors and biosensors due to its physical and electrochemical properties. This review discusses the role of graphene and graphene related materials for the improvement of the analytical performance of sensors and biosensors. This paper also provides an overview of recent graphene based sensors and biosensors (2012–2016), comparing their analytical perfor...
Biodegradation of polyethylene microplastics by the marine fungus zalerion mari...
Paço, Ana; Duarte, Kátia; Costa, João P. da; Santos, Patrícia S.M.; Pereira, R.; Pereira, M. E.; Freitas, Ana C.; Duarte, Armando C.Plastic yearly production has surpassed the 300milliontons mark and recycling has all but failed in constituting a viable solution for the disposal of plastic waste. As these materials continue to accumulate in the environment, namely, in rivers and oceans, in the form of macro-, meso-, micro- and nanoplastics, it becomes of the utmost urgency to find new ways to curtail this environmental threat. Multiple effo...
Bioactive polysaccharides extracts from sargassum muticumby high hydrostatic pr...
Rodrigues, Dina; Freitas, Ana C.; Queirós, Rui; Rocha-Santos, Teresa A. P.; Saraiva, Jorge A.; Gomes, Ana M. P.; Duarte, Armando C.Sargassum muticum is an important source of bioactive polysaccharides; hence, high hydrostatic pressure (HHP) was used to improve their extraction efficiency. Response surface methodology and a Box-Behnken full factorial design were employed to assess and optimize the effects of extraction conditions on the yield, total sugars, total sulfated sugars and antioxidant activity of S. muticum extracts. The extractio...
Effect of probiotic co-cultures on physico-chemical and biochemical properties ...
Pinto, Jorge M. S.; Sousa, Sérgio; Rodrigues, Dina M.; Malcata, Francisco X.; Duarte, Armando C.; Rocha-Santos, Teresa A. P.; Freitas, Ana C.Small ruminants' fermented probiotic milk is an alternative to fermented cows' milk, especially because of the monounsaturated/polyunsaturated fatty acid profiles. The technological and biochemical potential of Bifidobacterium and Lactobacillus co-cultures, with or without inulin, on goats' and ewes' milk was assessed. Microbial stability, lactose consumption, organic acid production, proteolytic parameters and...
Characterization of freezing effect upon stability of, probiotic loaded, calciu...
Sousa, Sérgio; Gomes, Ana M.; Pintado, Maria M.; Silva, José P.; Costa, Paulo; Amaral, Maria H.; Duarte, Armando C.; Rodrigues, DinaMicroencapsulation, utilizing different techniques and polymers, has been studied with the objective of maintaining probiotic viability in food matrices, protecting the cells from their detrimental environment, storage conditions andthe passage of gastrointestinal tract (GIT). The main objective of this study was to assess the effect of freezing at−20◦C upon probiotic alginate-calcium microparticles’ integrity ...
Disposable sensors for environmental monitoring of lead, cadmium and mercury
Duarte, Katia; Justino, Celine I. L.; Freitas, Ana C.; Gomes, Ana M. P.; Duarte, Armando; Rocha-Santos, Teresa A. P.Miniaturization is an increasing trend in the field of analytical chemistry as a response to the need to develop new analytical techniques for food, clinical, and environmental applications. There is therefore also an increasing trend towards the use of miniaturized disposable sensors, which are inexpensive and designed to be one-shot and do not require pre-treatment prior to use or cleaning between measurement...