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The dynamics of flower development in Castanea sativa Mill

Alhinho, Ana Teresa Talhinhas Barata; Ramos, Miguel Jesus Nunes; Alves, Sofia; Rocheta, Margarida; Morais-Cecílio, Leonor; Gomes-Laranjo, José

The sweet chestnut tree (<i>Castanea sativa</i> Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecul...


The Dynamics of Flower Development in Castanea sativa Mill.

Alhinho, Ana Teresa; Ramos, Miguel Jesus Nunes; Alves, Sofia; Rocheta, Margarida; Morais-Cecilio, Leonor; Gomes-Laranjo, José; Sobral, Rómulo

The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mech...


Portuguese wild grapevine genome re-sequencing (Vitis vinifera sylvestris)

Ramos, Miguel J. N.; Coito, João L.; Faísca-Silva, David; Cunha, Jorge; Costa, Maria Manuela Ribeiro; Amâncio, Sara; Rocheta, Margarida

The first genome of Vitis vinifera vinifera (PN40024), published in 2007, boosted grapevine related studies. While this reference genome is a suitable tool for the overall studies in the field, it lacks the ability to unveil changes accumulated during V. v. vinifera domestication. The subspecies V. v. sylvestris preserves wild characteristics, making it a good material to provide insights into V. v. vinifera do...


RNA editing in inflorescences of wild grapevine unveils association to sex and ...

Ramos, Miguel J. N.; Faísca-Silva, David; Coito, João L.; Cunha, Jorge; Silva, Helena Gomes; Viegas, Wanda; Costa, Maria Manuela Ribeiro; Amâncio, Sara

RNA editing challenges the central dogma of molecular biology, by modifying the genetic information at the transcription level. Recent reports, suggesting increased levels of RNA editing in plants, raised questions on the nature and dynamics of such events during development. We here report the occurrence of distinct RNA editing patterns in wild Vitis flowers during development, with twelve possible RNA editing...


Portuguese wild grapevine genome re-sequencing (Vitis vinifera sylvestris)

Ramos, Miguel J.N.; Coito, João L.; Faísca-Silva, David; Cunha, Jorge; Costa, M.Manuela R.; Amâncio, Sara; Rocheta, Margarida

The first genome of Vitis vinifera vinifera (PN40024), published in 2007, boosted grapevine related studies. While this reference genome is a suitable tool for the overall studies in the field, it lacks the ability to unveil changes accumulated during V. v. vinifera domestication. The subspecies V. v. sylvestris preserves wild characteristics, making it a good material to provide insights into V. v. vinifera do...


Vitis flower types: from the wild to crop plants

Coito, Joao L.; Silva, Helena G.; Ramos, Miguel J. N.; Cunha, Jorge; Eiras-Dias, Jose; Amancio, Sara; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Vitis vinifera can be divided into two subspecies, V. vinifera subsp. vinifera, one of the most important agricultural crops in the world, and its wild ancestor, V. vinifera subsp. sylvestris. Three flower types can be observed: hermaphrodite and female (on some varieties) in vinifera, and male or female flowers in sylvestris. It is assumed that the different flower types in the wild ancestor arose through spec...


Vitis flower sex specification acts downstream and independently of the ABCDE m...

Coito, João L.; Silva, Helena Sofia Gomes; Ramos, Miguel J. N.; Montez, Miguel; Cunha, Jorge; Amâncio, Sara; Costa, Maria M. R.; Rocheta, Margarida

The most discriminating characteristic between the cultivated Vitis vinifera subsp. vinifera and the wild-form Vitis vinifera subsp. sylvestris is their sexual system. Flowers of cultivars are mainly hermaphroditic, whereas wild plants have female and male individuals whose flowers follow a hermaphroditic pattern during early stages of development and later develop non-functional reproductive organs. In angiosp...


VviAPRT3 and VviFSEX: Two Genes Involved in Sex Specification Able to Distingui...

Coito, João L.; Ramos, Miguel J. N.; Cunha, Jorge; Silva, Helena Sofia Gomes; Amâncio, Sara; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex regio...


Deep analysis of wild Vitis flower transcriptome reveals unexplored genome regi...

Ramos, Miguel Jesus Nunes; Coito, João Lucas; Fino, Joana; Cunha, Jorge; Silva, Helena; Almeida, Patrícia Gomes; Costa, Maria Manuela Ribeiro

Key message RNA-seq of Vitis during early stages of bud development, in male, female and hermaphrodite flowers, identified new loci outside of annotated gene models, suggesting their involvement in sex establishment. Abstract The molecular mechanisms responsible for flower sex specification remain unclear for most plant species. In the case of V. vinifera ssp. vinifera, it is not fully understood what determine...


VviAPRT3 and VviFSEX: two genes involved in sex specification able to distingui...

Coito, João L.; Ramos, Miguel J.N.; Cunha, Jorge; Silva, Helena G.; Amâncio, Sara; Costa, Maria M.R.; Rocheta, Margarida

Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located in the sex regio...


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