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Sustainable synthesis and optimisation of ethyl oleate from high oleic acid was...

Soares, Ana; Fontes, Ana Luiza; Teixeira, Francisca; Costa, Paula; Pimentel, Lígia; Pintado, Manuela; Rodríguez-Alcalá, Luis Miguel

Ethyl oleate (EO) is a versatile compound with several industrial applications, such as a vaccine adjuvant, an emollient in cosmetics, and a key component in food products as an additive used for pretreatment in preservation processes such as drying, while preserving valuable nutrients. Ethyl oleate is primarily synthesised from edible oils, which raises concerns regarding competition with food production. This...


Microbial conjugated linolenic acid-enriched fermented milk using lipase-hydrol...

Fontes, Ana Luiza; Pimentel, Lígia L.; Monteiro, Maria João P.; Domingues, M. Rosário; Rodríguez-Alcalá, Luis Miguel; Gomes, Ana Maria

The bioactive conjugated linolenic acid (CLNA) can be microbiologically produced by different probiotic strains when in the presence of α-linolenic acid (α-LNA). Food matrices are a good vector, such as has been previously demonstrated with fermented milk enriched with microbial CLNA by Bifidobacterium breve DSM 20091 from lipase-hydrolyzed flaxseed oil. The aim of the present work was to further assess the nut...


The use of cecal samples from Wistar rats in in vitro fermentation to determine...

Salsinha, Ana Sofia; Cima, André; Araújo-Rodrigues, Helena; Viana, Sofia; Reis, Flávio; Coscueta, Ezequiel R.; Rodríguez-Alcalá, Luis Miguel

The influence of gut microbiota in the onset and development of several metabolic diseases, such as obesity and diabetes, has gained attention over the last few years. Indeed, diet plays an essential role in gut microbiota modulation. Western diet (WD), characterized by high-sugar and high-fat consumption, alters gut microbiome composition, diversity index, microbial relative levels, and functional pathways. Se...


Short-communication: study of fatty acid metabolites in microbial conjugated fa...

Fontes, Ana Luiza; Neves, Bruna; Conde, Tiago; Couto, Daniela; Pimentel, Lígia Leão; Rodríguez-Alcalá, Luis Miguel; Domingues, M. Rosário

Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk ferm...


Bigels as delivery systems of bioactive fatty acids present in functional edibl...

Machado, Manuela; Sousa, Sérgio Cruz; Rodríguez-Alcalá, Luis Miguel; Pintado, Manuela; Gomes, Ana Maria

Bioactive fatty acids possess several benefits for human health; however, these molecules show a reduced oxidative stability and consequently reduced bioavailability. This work aimed to develop novel bigels as a strategy to protect bioactive fatty acids present in three different vegetable oils with nutritional attributes (coconut oil, avocado oil, and pomegranate oil) during passage through the gastrointestina...


Study of the viability of using lipase-hydrolyzed commercial vegetable oils to ...

Fontes, Ana Luiza; Pimentel, Lígia; Soares, Ana Maria Silva; Domingues, M. Rosário; Rodríguez-Alcalá, Luis Miguel; Gomes, Ana Maria

This work studied the viability of using vegetable oils as precursor substrates to develop a dairy product enriched in microbial conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids. Hydrolysis of hempseed, flaxseed (FSO) and soybean (SBO) oils was tested with Candida rugosa (CRL), Pseudomonas fluorescens, or Pancreatic porcine lipases. FSO and SBO, previously hydrolyzed with CRL, were further select...


Study of the association between genotypic potential and linoleic acid toleranc...

Fontes, Ana Luiza; Pimentel, Lígia Leão; Calzada, Javier; Salsinha, Ana Sofia; Rodríguez, Juan Miguel; Gomes, Ana Maria; Arqués, Juan L.

Probiotic bacteria have been shown to produce the bioactive conjugated linoleic acid (CLA) from linoleic acid (LA), this being associated with a detoxifying mechanism. Primers employed in genotypic selection are generally species-specific. Therefore, this work aimed to identify CLA producers by previously screening genes encoding enzymes involved in LA isomerization with primers designed based on conserved moti...


The use of a human in vitro fermentation model to study the role of omega-3 and...

Salsinha, Ana Sofia; Cima, André; Araújo-Rodrigues, Helena; Rodríguez-Alcalá, Luis Miguel; Relvas, João B.; Pintado, Manuela


Fatty acids role on obesity induced hypothalamus inflammation: from problem to ...

Salsinha, Ana Sofia; Rodríguez-Alcalá, Luis Miguel; Relvas, João B.; Pintado, Manuela Estevez

Background: Obesity has currently reached a worldwide pandemic level, playing a central role in the development of non-communicable diseases and in health care burden. The available drugs for obesity have not achieved the required level of clinical effectiveness and have been associated with severe health side effects. Recent investigations suggest that obesity is more complex as it is associated with altered b...


CLA and CLNA ameliorate neuroinflammation and cellular oxidation related with w...

Salsinha, Ana Sofia; Socodato, Renato; Rodrigues, Artur; Rodríguez-Alcalá, Luis Miguel; Relvas, João B.; Pintado, Manuela E.


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