15 documents found, page 1 of 2
The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence...
Branco, Joana; Martins-Cruz, Cláudia; Rodrigues, Lisa; Silva, Raquel M.; Araújo-Gomes, Nuno; Gonçalves, Teresa; Miranda, Isabel M.; Rodrigues, Acácio G.Candida parapsilosis is an emergent opportunistic yeast among hospital settings that affects mainly neonates and immunocompromised patients. Its most remarkable virulence traits are the ability to adhere to prosthetic materials, as well as the formation of biofilm on abiotic surfaces. The Ndt80 transcription factor was identified as one of the regulators of biofilm formation by C. parapsilosis; however, its fun...
The transcription factor Ndt80 is a repressor of candida parapsilosis virulence...
Branco, Joana; Martins-Cruz, Cláudia; Rodrigues, Lisa; Silva, Raquel M.; Araújo-Gomes, Nuno; Gonçalves, Teresa; Miranda, Isabel M.; Rodrigues, Acácio G.Candida parapsilosis is an emergent opportunistic yeast among hospital settings that affects mainly neonates and immunocompromised patients. Its most remarkable virulence traits are the ability to adhere to prosthetic materials, as well as the formation of biofilm on abiotic surfaces. The Ndt80 transcription factor was identified as one of the regulators of biofilm formation by C. parapsilosis; however, its fun...
Antibacterial action mechanisms of honey: physiological effects of avocado, che...
Combarros-Fuertes, Patricia; Estevinho, Leticia M.; Teixeira-Santos, Rita; Rodrigues, Acácio G.; Pina-Vaz, Cidália; Fresno, José MaríaNumerous studies have explored the antibacterial properties of di erent types of honey from all around the world. However, the data available describing how honey acts against bacteria are few. The aim of this study was to apply a flow cytometry (FC) protocol to examine and characterize the primary e ects of three varieties of honey (avocado, chestnut and polyfloral) upon physiological status of Staphylococcus ...
Ultra-rapid flow cytometry assay for colistin MIC determination in Enterobacter...
Fonseca e Silva, Daniela; Andrade, Ferdinando F.; Gomes, Rosário; Silva-Dias, Ana; Martins-Oliveira, Inês; Pérez-Viso, Blanca; Ramos, Maria HelenaObjectives: Both EUCAST and CLSI recommend broth microdilution for antimicrobial susceptibility testing of colistin, but this method is cumbersome and takes 16-24 h to give results. Our objective was to evaluate a rapid quantitative colistin MIC susceptibility assay based on flow cytometry analysis (FASTcolistin MIC) in comparison with standard broth microdilution assay. Methods: One hundred and sixteen Gram-ne...
Evaluation of physiological effects induced by manuka honey upon staphylococcus...
Combarros-Fuertes, Patricia; Estevinho, Leticia M.; Teixeira-Santos, Rita; Rodrigues, Acácio G.; Pina-Vaz, Cidália; Fresno, José MaríaSeveral studies have explored the antimicrobial properties of manuka honey (MkH). However, the data available regarding antibacterial action mechanisms are scarcer. The aim of this study was to scrutinize and characterize primary e ects of manuka honey (MkH) upon the physiological status of Staphylococcus aureus and Escherichia coli (as Gram-positive and Gram-negative bacteria models, respectively), using flow ...
Flow cytometric antimicrobial susceptibility test for gram-negative bacilli dir...
Oliveira, Joana Santos; Santos, Rita Teixeira; Oliveira, Sofia Costa de; Dias, Ana Silva; Oliveira, Inês Martins; Rodrigues, Acácio G.Urinary tract infections (UTIs) are the most frequent infections in both hospitalized and community patients. These infections may provoke serious complications especially in children, pregnant human and elderly. The rate of recurrent UTIs is high and the increasing number of resistant bacteria is intensified by incorrect or excessive use of antimicrobial drugs. Consequently, rapid antimicrobial susceptibility ...
Screening of positive urines using Sysmex UF-1000i flow cytometer and identific...
Oliveira, Joana Santos; Santos, Rita Teixeira; Oliveira, Sofia Costa de; Dias, Ana Silva; Oliveira, Inês Martins; Rodrigues, Acácio G.Urinary tract infections (UTIs) are the most common infections both in hospitalized and community patients often leading to severe complications. Gram-negative bacilli are the most prevalent microorganisms often resistant to main antimicrobial agents. Microbiology diagnosis is necessary but take at least 48 hours to provide results. Sysmex UF-1000i is an automated urine flow cytometer that have been described a...
Blunted dynamics of adenosine A2A receptors is associated with increased suscep...
Rodrigues, Lisa; Miranda, Isabel M.; Andrade, Geanne M.; Mota, Marta; Cortes, Luísa; Rodrigues, Acácio G.; Cunha, Rodrigo A.; Gonçalves, TeresaOpportunistic gut infections and chronic inflammation, in particular due to overgrowth of Candida albicans present in the gut microbiota, are increasingly reported in the elder population. In aged, adult and young mice, we now compared the relative intestinal over-colonization by ingested C. albicans and their translocation to other organs, focusing on the role of adenosine A2A receptors that are a main stop si...
Development of cross-resistance by Aspergillus fumigatus to clinical azoles fol...
Faria-Ramos, Isabel; Farinha, Sofia; Neves-Maia, João; Tavares, Pedro Ribeiro; Miranda, Isabel M.; Estevinho, Leticia M.; Pina-Vaz, CidáliaBackground: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatu...
Environmental azole fungicide, prochloraz, can induce cross-resistance to medic...
Faria-Ramos, Isabel; Tavares, Pedro Ribeiro; Farinha, Sofia; Neves-Maia, João; Miranda, Isabel M.; Silva, Raquel M.; Estevinho, Leticia M.Acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. The main goal of this study was to assess the development of cross-resistance between agricultural and clinical azoles by Candida spp. An in vitro induction assay was performed, for a period of 90 days, with prochloraz (PCZ) - an agricultural antifungal. Afterward, the i...