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Optimizing bacterial nanocellulose production from eucalyptus bark: a circular ...

Rodrigues, Ana Cristina Costa; Martins, Daniela Sofia Rodrigues; Duarte, Maria Salomé Lira; Carvalho, Ricardo Emanuel Silva; Marques, Susana

The production cost of bacterial nanocellulose (BNC) is a major limitation to its widespread use. However, this limitation can be addressed by using alternative low-cost substrates and high-yield strains. Agro-industrial waste-derived substrates offer a cost-effective and sustainable solution, but their high organic load often requires additional downstream wastewater treatments. Here, we optimized static BNC p...


Bacterial cellulose in vitro uptake by macrophages, epithelial cells, and a tri...

Carvalho, Ricardo Emanuel Silva; Rodrigues, Patrícia M.; Martins, Daniela Sofia Rodrigues; Rodrigues, Ana Cristina Costa; Sampaio, Paula

Bacterial cellulose (BC) has a long-standing human consumption history in different geographies without any report of adverse effects. Despite its unique textural and functional properties, the use of BC in food products in Europe is still restricted due to concerns over its nanosize. Here, we evaluated the potential uptake of celluloses (from plant and microbial sources, processed using different blenders) by ...


Assessment of the gastrointestinal fate of bacterial nanocellulose and its toxi...

Rodrigues, Ana Cristina Costa; Costa, Liliana Marisa Barros; Silva-Carvalho, Ricardo; Mota, Renato; Duarte-Silva, Sara

Bacterial nanocellulose (BNC), a nanofibrillar polysaccharide produced by certain acetic acid bacteria, may be used in food, pharma and many other applications. As with other nanoscale celluloses, the oral toxicology of BNC is still controversial. Therefore, we aimed to assess whether BNC is absorbed at the intestine following administration in Wistar rats. This is, to our knowledge, the first attempt to track ...


Spatially explicit model for anaerobic co‐digestion facilities location and pre...

Coura, Renata D'arc; Alonso, Joaquim Mamede; Rodrigues, Ana Cristina Costa; Ferraz, Ana Isabel; Mouta, Nuno; Silva, Renato; Brito, António Guerreiro de

The high volumes of animal manure and sewage sludge, as a consequence of the development of intensive and specialized cattle dairy farms in peri-urban areas, pose challenges to local environmental quality and demands for systems innovation. Besides these negative impacts, energy recovery from biogas produced in anaerobic co-digestion processes should contribute to local sustainable development. This research co...


Bacterial cellulose wound dressing

Rodrigues, Ana Cristina Costa

Wounds, in particular traumatic (e.g. burns) and chronic ones, are a major cause of morbidity, impaired life quality and high health care costs. They often result in long hospitalization stays, taking up substantial health resources in developed countries. Conventional treatments are often painful, expensive and may increase the infection risk, compromising the treatments’ time and success. In recent years, the...


Combined pretreatment by ultrasound and struvite precipitation of raw substrate...

Coura, Renata D'arc; Rodrigues, Ana Cristina Costa; Alonso, Joaquim Mamede; Ferraz, Ana Isabel; Brito, Luis Miguel; Abrantes, João Carlos de Castro

The present study aimed to optimize the struvite chemical precipitation process in nitrogen-rich anaerobic co-digestion systems. Struvite precipitation experiments were carried out using a mixture of cattle slurry liquid fraction and sewage sludge, with and without ultrasound pretreatment. Marine salt or MgCl2 were used as magnesium source in NH4+:Mg2+ stoichiometric proportions of 1:1.5 and 1:3. Under the test...


4th International Symposium on Bacterial Nanocellulose (ISBNC 2019) - Book of A...

Gama, F. M.; Dourado, Fernando; Freire, Carmen; Silvestre, Armando; Rodrigues, Ana Cristina Costa


Celluclast and Cellic® CTec2: Saccharification / fermentation of wheat straw, s...

Rodrigues, Ana Cristina Costa; Haven, Mai Østergaard; Lindedam, Jane; Felby, Claus; Gama, F. M.

The hydrolysis/fermentation of wheat straw and the adsorption/desorption/deactivation of cellulases were studied using Cellic® CTec2 (Cellic) and Celluclast mixed with Novozyme 188. The distribution of enzymes cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and -glucosidase of the two formulations between the residual substrate and supernatant during the course of enzymatic hydrolysis and fermentation was ...


Biomass saccharification : development of strategies for enzyme recycling

Rodrigues, Ana Cristina Costa; Haven, Mai Østergaard; Lindedam, Jane; Felby, Claus; Gama, Miguel

In the present work the recycling of free enzymes after prehydrolysis and simultaneous saccharification and fermentation of pretreated wheat straw under a variety of conditions was investigated. It was found that a significant amount of active cellulase and glucosidase could be recovered by recycling the free cellulases the amount of free enzymes increase with its thermostability and hydrolytic efficiency. At 5...


Recombinant expression and purification of the antimicrobial peptide Magainin-2

Ramos, Reinaldo Rodrigues; Moreira, Susana Margarida Gomes; Rodrigues, Ana Cristina Costa; Gama, F. M.; Domingues, Lucília

Magainin-2 (MAG2) is a polycationic antimicrobial peptide isolated from the skin of the African clawed frog Xenopus laevis. It has a wide spectrum of antimicrobial activities against Gram-positive and Gram-negative bacteria, fungi and induces osmotic lysis of protozoa. MAG2 also possesses antiviral and antitumoral properties. These activities make this peptide a promising candidate for therapeutic applications....


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