18 documents found, page 1 of 2
Importância da utilização de ácido alfa lipóico (ALA) e catalase (CAT) no proce...
Silva, Luciana Mascena; Lunardi, Franciele Osmarini; Rodrigues, Giovanna Quintino; Girão, Virgínia Cláudia Carneiro; Rodrigues, Ana Paula RibeiroCryopreservation of preantral ovarian follicles is a promising prospect for the preservation of oocyte fertility and oocyte development or in the case of pathologies and therapeutics that may compromise follicular reserves. The great advantage of cryopreservation of ovarian tissue is that it is independent of the age and stage of the menstrual/estral cycle, and involves fewer ethical and social issues. However,...
Degeneration rate of preantral follicles in the ovaries of goats
Silva, José Roberto Viana; Ferreira, Marcos Antônio Leal; Costa, Sonia Helena Furtado; Santos, R.R; Carvalho, Fabíola Christian Almeida deThe degeneration rate of ovarian preantral follicles in goats, and the distribution in the follicular classes (primordial, primary or secondary) was assessed. Ovaries from adult goats were collected at a local slaughterhouse. To evaluate the morphology of the caprine preantral follicles in situ, one fragment from each ovary was fixed individually in Carnoy for 12 h, sectioned serially at a thickness of 7 μm and...
Effect of the interval of serial sections of ovarian tissue in the tissue chopp...
Lucci, Carolina Madeira; Amorim, Christiani Andrade; Báo, Sônia Nair; Figueiredo, José Ricardo; Rodrigues, Ana Paula Ribeiro; Silva, José Roberto VianaThe present work investigated the effect of the interval of serial sections of ovarian tissue on the number of isolated preantral follicles in the goat. Goat ovaries were cut in the tissue chopper at eight different intervals. The quality of isolated follicles were evaluated by histology and transmission electron microscopy. Best results were obtained when the ovaries were cut in the tissue chopper at intervals...
Study of preantral follicle population in situ and after mechanical isolation f...
Lucci, Carolina Madeira; Amorim, Christiani Andrade; Rodrigues, Ana Paula Ribeiro; Figueiredo, José Ricardo; Báo, Sônia NairThe purposes of this study were to estimate the population of caprine preantral follicles, and to evaluate quantitatively and qualitatively the efficiency of a specific mechanical method for the isolation of preantral follicles from mixed breed goats at different reproductive stages. On average, 37,646±4277 preantral follicles were present in goat ovaries, and 13,631±2399 preantral follicles were obtained after...
Tutela antecipatória contra o poder público
Rodrigues, Ana Paula RibeiroOrientador: Manoel Caetano Ferreira Filho; Monografia (graduação) - Universidade Federal do Paraná,Setor de Ciências Jurídicas, Curso de Graduação em Direito; A tutela antecipatória propicia a distribuição do ônus do tempo no processo, sendo instrumento de concretização do equilíbrio abstrato entre os princípios da segurança jurídica e da inafastabilidade da tutela jurisdicional. É o ordenamento constitucional,...
Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryop...
Wanderleya, Livia Schell; Luza, Hiédely Kenia Machado; Faustino, Luciana Rocha; Lima, Isadora Machado Teixeira; Lopes, Cláudio Afonso PinhoThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing,...
Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
Rodrigues, Ana Paula Ribeiro; Amorim, C.A; Costa, Sonia Helena Furtado; Matos, Maria Helena Tavares de; Santos, R.R.; Lucci, Carolina MadeiraCryopreservation of ovarian tissue may be a potential alternative for the conservation of genetically superior animals, including high milk- and meat-producing goat breeds. However, until now, no information was available concerning the cryopreservation of preantral follicles (PF) enclosed in caprine ovarian tissue. The objective of the present study was to evaluate the structural and ultrastructural characteri...
Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine ...
Araújo, Valdevane Rocha; Silva, Cleidson Manoel Gomes da; Magalhães, Deborah M.; Silva, Gerlane Modesto da; Báo, Sônia Nair; Silva, José Roberto VianaO presente trabalho foi conduzido de modo a se verificar o efeito de diferentes concentrações da BMP-7 no desenvolvimento in vitro de folículos pré-antrais caprinos. Fragmentos de tecido cortical ovariano caprino foram cultivados por 1 ou 7 dias em Minimum Essential Medium (MEM+) suplementado com diferentes concentrações de BMP-7 (1, 10, 50 ou 100ng/ml). Os fragmentos não cultivados ou aqueles cultivados por 1 ...
Característica histológica, ultra-estrutural e produção de nitrito de folículos...
Bruno, J.B.; Lima-Verde, Isabel Bezerra; Martins, Fabricio Sousa; Matos, Maria Helena Tavares; Lopes, C.A.P.; Maia-Jr, J.E.; Báo, Sônia NairAvaliou-se o efeito da adição de diferentes tipos e concentrações de soro sobre o desenvolvimento e a sobrevivência de folículos ovarianos pré-antrais (FOPA) caprinos in vitro. Além disso, verificou-se a relação entre as concentrações de nitrito presentes no meio de cultivo e a viabilidade folicular. Cada par ovariano foi dividido em 29 fragmentos, sendo um destinado ao controle. Os fragmentos foram cultivados ...
Morphologic, viability and ultrastructural analysis of vitrified sheep preantra...
Lunardi, Franciele Osmarini; Araújo, Valdevane Rocha; Faustino, Luciana Rocha; Carvalho, Adeline de Andrade; Gonçalves, Raphael Fernando BragaThe main objective was to compare the efficiency of vitrification techniques and solutions on the preservation of morphology, ultrastructure and viability of sheep preantral follicles enclosed in ovarian tissue fragments. The fragments were cryopreserved by using macrotube vitrification (MTV), solid-surface vitrification (SSV) or conventional vitrification (CV). These techniques were combined with one of the si...