9 documents found, page 1 of 1

Stromal vascular fraction cell sheets angiogenic potential for tissue engineeri...

One of the biggest concerns in the Tissue Engineering field is the correct vascularization of engineered constructs. Strategies involving the use of endothelial cells are promising but adequate cell sourcing and neo-vessels stability are enduring challenges. In this work, we propose the hypoxic pre-conditioning of the stromal vascular fraction (SVF) of human adipose tissue to obtain highly angiogenic cell sheet...

Adipose tissue-derived stem cells do not elicit alloreactivity after chondrogen...

Mesenchymal stem cells lack or express low levels of major histocompatibility complex (MHC) class II and other immunoactive costimulatory molecules rendering them â â immunoincompetentâ â . We have shown that human adipose tissue-derived stem cells (hASCs) implanted subcutaneously in mice did not elicit adverse immune responses 1. In this study we hypothesised that undifferentiated hASCs, and derived osteoblast...

Hind limb ischemia in type 1 diabetic mice as useful tool to evaluate the neova...

Hind-limb ischemia has been used in type 1 diabetic mice to evaluate treatments for peripheral arterial disease or mechanisms of vascular impairment in diabetes [1]. Vascular deficiency is not only a pathophysiological condition, but also an obvious circumstance in tissue regeneration and in tissue engineering and regenerative medicine (TERM) strategies. We performed a pilot experiment of hind-limb ischemia in ...

Stromal vascular fraction from adipose tissue and cell sheet engineering to bui...

Vascularization holds the gold key for the effective survival and engraftment of complex engineered tissues and organs for Tissue Engineering and Regenerative Medicine. The lack of adequate vascularization post-transplantation often results in cell necrosis and ultimate failure and rejection of the engineered construct. Herein, we propose a strategy capable of surpassing this obstacle. Harnessing easy accessibl...

Xenogeneic M2-polarization of Macrophages by undifferentiated and differentiate...

Mesenchymal stem cells (MSCs) are considered to be â â immunologically privileged.â â In a previous work when human adipose tissue-derived stem cells (hASCs) subcutaneously implanted in mice we did not identify an adverse host response1. Recently, it was shown that tissue regeneration could benefit from the polarization of M2 macrophages subpopulations 2. In this study we hypothesised that undifferentiated hASC...

Demandas atuais e futuras sob incertezas e adaptações de longo prazo em bacias ...

Human adipose stem cells cell sheet constructs impact epidermal morphogenesis i...

Among the wide range of strategies to target skin repair/regeneration, tissue engineering (TE) with stem cells at the forefront, remains as the most promising route. Cell sheet (CS) engineering is herein proposed, taking advantage of particular cell-cell and cell-extracellular matrix (ECM) interactions and subsequent cellular milieu, to create 3D TE constructs to promote full-thickness skin wound regeneration. ...

Tissue engineered constructs based on human adipose tissue derived stem cells a...

The combination of natural-origin materials with mesenchymal stem cells (MSCs) from different sources have been suggested as suitable constructs for Tissue Engineering and Regenerative Medicine (TERM). Considering the importance of the host immune response to implanted tissue engineered constructs (TECs) and the immunomodulatory role assigned to MSCs and to adipose derived stem cells (ASCs) in particular, this ...

Study of the one dimensional and transient bioheat transfer equation: Multi-lay...

: In this work we derive an analytical solution given by Bessel series to the transient and one-dimensional (1D) bioheat transfer equation in a multi-layer region with spatially dependent heat sources. Each region represents an independent biological tissue characterized by temperature-invariant physiological parameters and a linearly temperature dependent metabolic heat generation. Moreover, 1D Cartesian, cyli...