13 documents found, page 1 of 2
Cryogenic, but not hypothermic, preservation disrupts the extracellular matrix ...
Ribeiro, Sara Cristina Freitas; Carvalho, A. F.; Rodrigues, Daniel Barreira; Martins, L.; Pires, R. A.; Mendes, V. M.; Manadas, B.; Jarnalo, M.Cell sheet (CS)-based approaches hold significant potential for tissue regeneration, relying on the extracellular matrix (ECM) for success. Like in native tissues, the ECM provides structural and biochemical support for cellular homeostasis and function. Effective preservation strategies that maintain ECM integrity are critical to enhance the therapeutic potential of CS-based approaches. While cryogenic and hyp...
Modelling the complex nature of the tumor microenvironment: 3D tumor spheroids ...
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.Cancer remains a serious burden in society and while the pace in the development of novel and more effective therapeutics is increasing, testing platforms that faithfully mimic the tumor microenvironment are lacking. With a clear shift from animal models to more complex in vitro 3D systems, spheroids emerge as strong options in this regard. Years of development have allowed spheroid-based models to better repro...
How are natural-based polymers shaping the future of cancer immunotherapy—A review
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.With the increasing knowledge of cancer pathophysiology, new therapeutics based on the modulation of the immune system have been developed, overcoming many of the disadvantages of traditional pharmaceuticals. Several immunotherapy systems have in fact become the preferred treatments to tackle particular types of cancer. Despite these impressive clinical results, issues, such as biomolecule susceptibility to pro...
Bioengineering strategies for cancer therapy and modelling
Rodrigues, Daniel BarreiraCancer is a global pandemic with a high incidence among the world population and effective treatments are for the most part elusive. The tumor microenvironment is a highly complex and heterotypic mixture of cells that interact to regulate central control mechanisms, driving immunosuppression and promoting both survival and invasion of cancer cells into surrounding tissues. It has been this complexity that has m...
How are natural-based polymers shaping the future of cancer immunotherapy: a re...
Rodrigues, Daniel Barreira; Reis, R. L.; Pirraco, Rogério P.With the increasing knowledge of cancer pathophysiology, new therapeutics based on the modulation of the immune system have been developed, overcoming many of the disadvantages of traditional pharmaceuticals. Several immunotherapy systems have in fact become the preferred treatments to tackle particular types of cancer. Despite these impressive clinical results, issues, such as biomolecule susceptibility to pro...
Spongy-like hydrogels prevascularization with the adipose tissue vascular fract...
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIn vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important...
Highly tailorable gellan gum nanoparticles as a platform for the development of...
Rodrigues, Daniel Barreira; Moreira, Helena R.; Cerqueira, Mariana T.; Marques, A. P.; Castro, António G.; Reis, R. L.; Pirraco, Rogério P.Background: T cell priming has been shown to be a powerful immunotherapeutic approach for cancer treatment in terms of efcacy and relatively weak side efects. Systems that optimize the stimulation of T cells to improve therapeutic efcacy are therefore in constant demand. A way to achieve this is through artifcial antigen presenting cells that are complexes between vehicles and key molecules that target relevant...
Integrin-specific hydrogels for growth factor-free vasculogenesis
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIntegrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascu...
Dendrimers: breaking the paradigm of current musculoskeletal autoimmune therapies
Rodrigues, Daniel Barreira; Oliveira, Joaquim M.; Santos, T. C.; Reis, R. L.The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that, regardless of some good results in clinical outcomes, are well known for several systemic sec...
Stem cell-containing hyaluronic acid-based spongy hydrogels for integrated diab...
Silva, Lucília Pereira; Santos, T. C.; Rodrigues, Daniel Barreira; Pirraco, Rogério P.; Cerqueira, Mariana Teixeira; Reis, R. L.; Correlo, V. M.The detailed pathophysiology of diabetic foot ulcers is yet to be established and improved treatments are still required. We propose a strategy that directs inflammation, neovascularization, and neoinnervation of diabetic wounds. Aiming to potentiate a relevant secretome for nerve regeneration, stem cells were precultured in hyaluronic acid-based spongy hydrogels under neurogenic/standard media before transplan...