4 documents found, page 1 of 1
Toward cost-effective production of recombinant human serum albumin for use in ...
Rodrigues, Diana Isabel Gomes; Adalsteinsson, Heidar Már; Pinto, Lavínia; Aguiar, Tatiana Quinta; Oliveira, Carla; Abrunhosa, Luís; Domingues, LucíliaHuman serum albumin (HSA) is a monomeric multidomain macromolecule with wide clinical and biotechnological applications. Thus, there is a high demand for HSA, which is increasing due to new emerging applications. Among these, the extraordinary ligand binding capacity of HSA has been recently explored to capture mycotoxins from food and feed matrices. However, HSA is currently produced commercially primarily fro...
High-throughput micro-plate fluorescence quenching for screening the binding of...
Adalsteinsson, Heidar Már; Pinto, Lavínia; Rodrigues, Diana Isabel Gomes; Domingues, Lucília; Vicente, António A.; Aguiar, Tatiana QuintaFilamentous fungi, which commonly contaminate food and animal feed, produce toxic metabolites called mycotoxins. Legal limits on their maximum levels in food require food producers to carry out highly specific analytical tests for mycotoxins, such as Ochratoxin A (OTA), patulin (PAT) and zearalenone (ZEN). Serum albumins are known to bind some mycotoxins, presenting an opportunity for the development of novel c...
Evaluation of ochratoxin a (OTA) interaction with recombinant domain II of bovi...
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana Isabel Gomes; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaOchratoxin A (OTA) is a mycotoxin that raises food and feed safety concerns, existing legal limits worldwide for its presence in foodstuffs and beverages. Immuno-affinity columns (IACs) are typically used to capture, clean-up and pre-concentrate OTA from food samples before quantification by high- performance liquid chromatography, but their price limits their application. Serum albumins form stable complexes w...
Characterization of cell factories capable of overproducing phenolic compounds
Rodrigues, Diana Isabel GomesOs compostos fenólicos são metabolitos secundários das plantas com diversas atividades biológicas e potencialmente terapêuticas, como antioxidante e anti-inflamatória. Tirosol, hidroxitirosol e salidrosina pertencem à classe de compostos fenólicos feniletanóides, encontrados em plantas como a oliveira, o chá verde e a planta Rhodiola, e apresentam atividade antioxidante e efeitos cardioprotetores. Os métodos tr...