63 documents found, page 1 of 7
Akkermansia muciniphila and Parabacteroides distasonis synergistically protect ...
Gaifem, Joana; Mendes-Frias, Ana; Wolter, Mathis; Steimle, Alex; Garzón, Maria Jose; Ubeda, Carles; Nobre, Clarisse; González, Abigail; Pinho, Salomé S.The contribution of the gut microbiome to the balance between homeostasis and inflammation is widely known. Nevertheless, the etiology of inflammatory bowel disease, which is known to be influenced by genetics, immune response, and environmental cues, remains unclear. Unlocking novel players involved in the dictation of a protective gut, namely, in the microbiota component, is therefore crucial to develop novel...
Interacting with hemoglobin: Paracoccidioides spp. recruits hsp30 on its cell s...
de Souza, Aparecido Ferreira; Tomazett, Mariana Vieira; Freitas e Silva, Kleber Santiago; de Curcio, Juliana Santana; Pereira, Christie Ataides<i>Paracoccidioides</i> spp. are thermally dimorphic fungi that cause paracoccidioidomycosis and can affect both immunocompetent and immunocompromised individuals. The infection can lead to moderate or severe illness and death. <i>Paracoccidioides</i> spp. undergo micronutrients deprivation within the host, including iron. To overcome such cellular stress, this genus of fungi responds in multiple ways, such as ...
Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transfo...
Nora, Luísa Czamanski; Gonçales, Relber Aguiar; Martins-Santana, Leonardo; Ferreira, Beatriz Henriques; Rodrigues, Fernando José dos SantosWe present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for ye...
Predictors and outcomes of disseminated tuberculosis in an intermediate burden ...
Meira, L.; Chaves, Catarina; Araújo, D.; Almeida, L.; Boaventura, R.; Ramos, A.; Carvalho, T.; Osório, Nuno S.; Castro, António G.Setting: University-affiliated hospital located in Porto, North Portugal, an area with a low to intermediate incidence of tuberculosis (TB). Objective: To identify predictors and outcomes of disseminated TB (dTB). Design: A cohort of patients diagnosed with TB between 2007 and 2013 was retrospectively analysed. Patients with dTB criteria were characterized and compared to single organ TB cases. Factors independ...
Ploidy determination in the pathogenic fungus Sporothrix spp.
Ferreira, Beatriz H.; Ramírez-Prado, Jorge H.; Neves, Gabriela W. P.; Torrado, Egídio; Sampaio, Paula; Felipe, Maria Sueli S.; Vasconcelos, Ana TerezaThe pathogenic clade of the Sporothrix genus comprises the etiological agents of sporotrichosis, a worldwide emergent disease. Despite the growing understanding of their successful pathogen traits, there is little information on genome sizes and ploidy within the genus. Therefore, in this work, we evaluated the ploidy of four species of the Sporothrix genus, specifically Sporothrix brasiliensis, Sporothrix sche...
The influence of genetic stability on Aspergillus fumigatus virulence and azole...
Reis, Thaila Fernanda dos; Silva, Lilian Pereira; Castro, Patrícia Alves; Lima, Pollyne Borborema Almeida de; Carmo, Rafaela Andrade doGenetic stability is extremely important for the survival of every living organism, and a very complex set of genes has evolved to cope with DNA repair upon DNA damage. Here, we investigated the Aspergillus fumigatus AtmA (Ataxia-telangiectasia mutated, ATM) and AtrA kinases, and how they impact virulence and the evolution of azole resistance. We demonstrated that A. fumigatus atmA and atrA null mutants are hap...
The Aspergillus nidulans pyruvate dehydrogenase kinases are essential to integr...
Ries, Laure Nicolas Annick; Assis, Leandro José de; Rodrigues, Fernando José dos Santos; Caldana, Camila; Rocha, Marina Campos; Malavazi, IranThe pyruvate dehydrogenase complex (PDH), that converts pyruvate to acetyl-coA, is regulated by pyruvate dehydrogenase kinases (PDHK) and phosphatases (PDHP) that have been shown to be important for morphology, pathogenicity and carbon source utilization in different fungal species. The aim of this study was to investigate the role played by the three PDHKs PkpA, PkpB and PkpC in carbon source utilization in th...
L-Threonine Supplementation During Colitis Onset Delays Disease Recovery
Gaifem, Joana; Gonçalves, Luís G.; Dinis-Oliveira, Ricardo J.; Cunha, Cristina; Carvalho, Agostinho; Torrado, EgídioDietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most ad...
Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive...
Gonçalves, Samuel Martins; Lagrou, Katrien; Rodrigues, Claudia S.; Campos, Claudia F.; Bernal-Martinez, Leticia; Rodrigues, Fernando José dos SantosBackground: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced reso...
Drivers of genetic diversity in secondary metabolic gene clusters within a fung...
Lind, Abigail L.; Wisecaver, Jennifer H.; Lameiras, Catarina; Wiemann, Philipp; Palmer, Jonathan M.; Keller, Nancy P.Filamentous fungi produce a diverse array of secondary metabolites (SMs) critical for defense, virulence, and communication. The metabolic pathways that produce SMs are found in contiguous gene clusters in fungal genomes, an atypical arrangement for metabolic pathways in other eukaryotes. Comparative studies of filamentous fungal species have shown that SM gene clusters are often either highly divergent or uniq...