143 documents found, page 1 of 15
Improving fructooligosaccharide production via sacC gene deletion in Zymomonas ...
Braga, Adelaide Correia; Maia, Ana Benedita; Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Costa, João Manuel RainhaFructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are produced from sucrose by the action of fructosyltransferase or b-fructofuranosidase enzymes. However, this process often yields low conversion rates and results in impure mixtures due to the release of high levels of glucose. Zymomonas mobilis is a well-known ethanol-...
De novo production of prenylnaringenin compounds by a metabolically engineered ...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Prenylnaringenin (PN) compounds, namely 8-prenylnaringenin (8-PN), 3-prenylnaringenin (3-PN), and 6-prenylnaringenin (6-PN), are reported to have several interesting bioactivities. This study aimed to validate a biosynthetic pathway for de novo production of PN in Escherichia coli. A previously optimized E. coli chassis capable of efficiently de novo producing naringenin was used to evaluate eleven prenyltransf...
Microbial production of caffeic acid
Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Caffeic acid is a hydroxycinnamic acid mostly produced in plants although its microbial production has also been reported. This compound presents several biological activities and potential therapeutic properties. Additionally, it can be a precursor or intermediary of various relevant compounds. Current production methods include the inefficient, expensive, and not environmentally friendly extraction from plant...
Microbial production of naringenin
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Naringenin is a plant natural compound that belongs to the flavonoid subclass of the polyphenolic compounds. It has several recognized health benefits, namely, antioxidant, anti-inflammatory, anticancer, and antiviral. Given these reported health benefits, an increasing interest in its utilization and incorporation by the pharmaceutical and nutraceutical industries in medicines and food supplements has emerged....
Microbial production of curcumin
Costa, João Manuel Rainha; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Curcumin, a polyphenol produced by turmeric (Curcuma longa), has attracted increased attention due to its potential as a novel cancer-fighting drug. However, to satisfy the required curcumin demand for health-related studies, high purity curcumin preparations are required, which are difficult to obtain and are very expensive. Curcumin and other curcuminoids are usually obtained through plant extraction. However...
De novo production of xanthohumol by a metabolically engineered Escherichia coli
Gomes, Daniela Filipa Correia; Santos, Joana; Venâncio, Armando; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Xanthohumol is a prenylflavonoid from hops with relevant bioactivities. Microbial production has emerged as a sustainable and potentially economic solution to produce it. Herein, we constructed a pathway for the de novo production of xanthohumol in Escherichia coli. Since the xanthohumol pathway depends on the availability of dimethylallyl pyrophosphate (DMAPP) and S-adenosylmethionine (SAM), SAM synthase (metK...
Engineering heat-inducible biosynthetic pathways in Escherichia coli for stress...
Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.The heat shock response of Escherichia coli offers a powerful tool to regulate gene expression under thermal stress. In this work, we explore and engineer this natural mechanism for two complementary purposes: the development of stress-responsive genetic tools and the biosynthesis of high-value therapeutic compounds. First, we identified and characterized E. coli heat shock promoters (from the ibpA, dnaK, and f...
CRISPR-Cas12a-driven engineering of DMAPP and SAM pathways in E. coli for de no...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Prenylflavonoids, including xanthohumol and prenylnaringenin (PN) (3-PN, 6-PN, and 8-PN), have been reported as bioactive compounds. However, their low accumulation in plants limits its broader utilization. We engineered Escherichia coli strains for the de novo biosynthesis of PNs and xanthohumol. Stepwise optimization was performed to construct naringenin chalcone and naringenin pathways. E. coli M-PAR-121 exp...
Exploiting CRISPR-Cas12a to engineer DMAPP/SAM pathways in Escherichia coli tow...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Prenylflavonoids are flavonoid derived compounds with interesting bioactivities, namely estrogenic and anticancer. Among them, xanthohumol and prenylnaringenin (PN) isomers, including 3-prenylnaringenin (3-PN), 6-prenylnaringenin (6-PN), and 8-prenylnaringenin (8-PN) have attracted special interest. Due to their low production and accumulation in plants, the construction of microbial cell factories able to prod...
Exploiting synthetic biology for the production of prenylflavonoids in Escheric...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Prenylflavonoids are flavonoids bearing a prenylated sidechain. Among them, xanthohumol and prenylnaringenin (PN) isomers, including 3-prenylnaringenin (3-PN), 6-prenylnaringenin (6-PN), and 8-prenylnaringenin (8-PN) have attracted interest due to their bioactive properties. However, their low accumulation in plants limits their utilization. The construction of microbial cell factories able to produce them appe...