26 documents found, page 1 of 3
In vitro effects of an Acanthophora muscoides (Ceramiales, Rhodophyta) native a...
Rodrigues, José Ariévilo Gurgel; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria BarrosThe structural complexity of the agaran type-sulfated polysaccharides (SPs) found in Acanthophora muscoides limits its investigation as anticoagulant alternative to heparin which induces clot complications. This study was extended to evaluate the properties of a SPs fraction and its alkali/desulfated derivatives on an intrinsic pathway-induced thrombin generation (TG) continuous model using 60-fold diluted norm...
Sulfated agaran with 4,6-pyruvated form from red seaweed Acanthophora muscoides...
Rodrigues, José Ariévilo Gurgel; Feitosa , Johnny Peter Macedo; Soares, Sandra de Aguiar; Benevides, Norma Maria BarrosIn vitro studies have described the sulfated agaran from Acanthophora muscoides as an intrinsic inhibitor of thrombin generation (TG), but not in ex vivo assay. This investigation partially characterized a pyruvate fraction with in vitro and ex vivo effects on an intrinsic/extrinsic pathway-induced thrombin generation (TG) continuous model using 36 or 60-fold diluted mice or defibrinated, normal human plasma. F...
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant sup...
Nascimento, Renata Vieira do; Pereira, Vanessa Alves; Almeida-Monteiro, Priscila Silva; Sales, Yara Silvino; Araújo, Ianna Wivianne Fernandes deThe study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2...
Extração, fracionamento, purificação e atividade anticoagulante de polissacaríd...
Rodrigues, José Ariévilo GurgelA alga marinha verde Caulerpa racemosa pertence é ordem Caulerpales, apresentando vesículas semelhantes a cachos de uva na região ereta e um talo rizomatoso na região basal. Nesta espécie, verifica-se a presença de polissacarideos sulfatados (PS), os quais são compostos muito estudados atualmente com possíveis usos nas ciências biomédicas e na biotecnologia. Este trabalho teve como propósito extrair, purificar ...
Molecular characteristics of polysulfated fractions from the green seaweed Caul...
Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de SouzaCaulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAE-cellulose chromatography, with d...
Extraction, physical-chemical characterization and in vitro inhibitory potentia...
Rodrigues, José Ariévilo Gurgel; Barcellos, Priscila Gomes; Glauser, Bianca Fernandes; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria BarrosThe biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoag...
Structural and physical-chemical analyses of sulfated polysaccharides from the ...
Barcellos, Priscila Gomes; Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Araújo, Ianna Wivianne Fernandes deUlva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, gluco...
In vitro activities of kappa-carrageenan isolated from red marine alga Hypnea m...
Souza, Ricardo Basto; Frota, Annyta Fernandes; Silva, Joana; Alves, Celso; Neugebauer, Agnieszka Zofia; Pinteus, Susete; Rodrigues, José Ariévilo GurgelThis study assessed the antioxidant, antimicrobial, anticancer and neuroprotective activities of the kappa(k)-carrageenan isolated from the red alga Hypnea musciformis (Hm-SP). The chemical spectrum of the k-carrageenan fromHm-SPwas confirmed by Fourier transform infrared (FT-IR) spectroscopy.Hm-SP revealed an antibacterial and antifungal action against Staphylococcus aureus and Candida albicans, respectively. ...
An anti-dengue and anti-herpetic polysulfated fraction isolated from the coenoc...
Rodrigues, José Ariévilo Gurgel; Eloy, Ygor Raphael Gomes; Vanderlei, Edfranck de Souza Oliveira; Cavalcante, Jéssica FigueiredoThe antiviral potency of Caulerpa cupressoides (Chlorophyta) sulfated polysaccharidic fractions (Cc-SP1→3) associated with thrombin generation (TG) assay is unknown. This study analyzed the structure of Cc-SP1 and its effects against herpes viruses (HSV-1 and HSV-2), dengue-virus (DENV-1), human-metapneumovirus (HMPV) and adenovirus (AdV) in Vero and mosquito C6/36 cell-lines and as inhibitor of TG in 60-fold d...
In vitro inactivation of thrombin generation by polysulfated fractions isolated...
Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de SouzaPharmacological efficacy of Caulerpa racemosa (Chlorophyta) sulfated polysaccharidic (SPs) fractions (F I→III) on models of coagulation and inflammation has been demonstrated, but not their effects on thrombin generation (TG). This study examined fractions for composition and physical-chemical characteristics and in vitro inactivation of TG by F I and F II in 60-fold diluted human plasma using continuous metho...