496 documents found, page 1 of 50
Improving fructooligosaccharide production via sacC gene deletion in Zymomonas ...
Braga, Adelaide Correia; Maia, Ana Benedita; Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Costa, João Manuel RainhaFructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are produced from sucrose by the action of fructosyltransferase or b-fructofuranosidase enzymes. However, this process often yields low conversion rates and results in impure mixtures due to the release of high levels of glucose. Zymomonas mobilis is a well-known ethanol-...
Liposomal formulations for efficient delivery of a novel, highly potent pyrimid...
Teixeira, Sofia; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, Ana Rita Oliveira; Rodrigues, L. R.; Castanheira, Elisabete M. S.Background/Objectives: Cancer is one of the deadliest diseases worldwide. Despite the existing treatments, the adverse side effects and the increasing drug resistance to the current therapies lead to a reduced quality of life for patients and poor prognosis. The pyrimido[5,4-d]pyrimidine compound (PP) was identified as a promising new anticancer drug due to its potent activity against colorectal and triple-nega...
Engineered shape-tunable copper-coordinated nanoparticles for macrophage reprog...
Gao, Han; Cheng, Ruoyu; Cardoso, Inês; Lobita, Maria; Pacheco-Fernández, Idaira; Bártolo, Raquel; Rodrigues, L. R.; Hirvonen, Jouni; Santos, Hélder A.The immune system safeguards as primary defense by recognizing nanomaterials and maintaining homeostasis, gaining a deeper understanding of these interactions may change the treating paradigm of immunotherapy. Here, we adopted copper as the principal component of nanoparticles (NPs), given its features of coordination with different benezenecarboxylate ligands to form metalorganic frameworks and complexes with ...
Modulation of enzymatic activity by moderate electric fields: perspectives for ...
Albuquerque, Tiago; Pereira, Ricardo Nuno Correia; Silvério, Sara Isabel Cruz; Rodrigues, L. R.Modulating enzymatic activity through physical strategies is increasingly recognized as a powerful approach to optimizing biocatalytic processes in food and biotechnology applications. Cellobiose 2-epimerase (C2E), a key enzyme for synthesizing epilactose, a non-digestible disaccharide with established prebiotic effects, is gaining relevance in functional foods. Emerging strategies, such as the application of m...
Understanding lactoferrin multifunctionality by analyzing the full spectrum of ...
Ferreira, Sofia; Guedes, Joana P.; Rocha, Juliana F.; Sousa, Sérgio F.; Rodrigues, L. R.; Côrte-Real, Manuela; Soares, Pedro; Cátia S. PereiraLactoferrin (LF) is a multifunctional protein involved in critical biological processes such as immune response, antimicrobial activity, and iron regulation [1]. Its diverse roles include inhibiting inflammation, infections, and carcinogenesis [2], as well as modulating cellular processes by targeting proton-pumping ATPases such as V-ATPases in cancer cells [3], F- ATPases in bacteria [4], and P-ATPases in yeas...
De novo production of prenylnaringenin compounds by a metabolically engineered ...
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Scrutton, Nigel S.; Rodrigues, L. R.Prenylnaringenin (PN) compounds, namely 8-prenylnaringenin (8-PN), 3-prenylnaringenin (3-PN), and 6-prenylnaringenin (6-PN), are reported to have several interesting bioactivities. This study aimed to validate a biosynthetic pathway for de novo production of PN in Escherichia coli. A previously optimized E. coli chassis capable of efficiently de novo producing naringenin was used to evaluate eleven prenyltransf...
Microbial production of caffeic acid
Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Caffeic acid is a hydroxycinnamic acid mostly produced in plants although its microbial production has also been reported. This compound presents several biological activities and potential therapeutic properties. Additionally, it can be a precursor or intermediary of various relevant compounds. Current production methods include the inefficient, expensive, and not environmentally friendly extraction from plant...
Microbial production of naringenin
Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Naringenin is a plant natural compound that belongs to the flavonoid subclass of the polyphenolic compounds. It has several recognized health benefits, namely, antioxidant, anti-inflammatory, anticancer, and antiviral. Given these reported health benefits, an increasing interest in its utilization and incorporation by the pharmaceutical and nutraceutical industries in medicines and food supplements has emerged....
Microbial production of curcumin
Costa, João Manuel Rainha; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.Curcumin, a polyphenol produced by turmeric (Curcuma longa), has attracted increased attention due to its potential as a novel cancer-fighting drug. However, to satisfy the required curcumin demand for health-related studies, high purity curcumin preparations are required, which are difficult to obtain and are very expensive. Curcumin and other curcuminoids are usually obtained through plant extraction. However...
Valorisation of olive oil by-products into pectic- and glucuronoxylo-oligosacch...
Cordeiro, Ana; Fernandes, Andreia; Silvério, Sara Isabel Cruz; Coelho, Elisabete; Coimbra, Manuel A.; António M. Peres; Rodrigues, L. R.Olive pomace (OLP) and stones (OLS) are key by-products of olive oil production, rich in lignocellulose and pectin, making them viable substrates for prebiotic oligosaccharide (OS) production. This study evaluated the chemical composition of OLP and OLS powders (OLPp and OLSp) and their potential for OS production through one-step fermentation using recombinant Bacillus subtilis 3610. Both substrates had compar...