20 documents found, page 1 of 2
Effect of vapor-phase oregano essential oil on resistant Candida species biofil...
Fernandes, Liliana Sousa; Costa, Raquel; Silva, Sónia Carina; Henriques, Mariana; Costa-de-Oliveira, Sofia; Rodrigues, M. ElisaThis work presents a new approach to the application of essential oils, exposure to the vapor phase, which can be considered a first approach for the development of a complementary or alternative form of vulvovaginal candidiasis (VVC) treatment. VVC is a significant infection caused by Candida species and remains a common disease that affects millions of women every year. Vulvovaginal candidiasis (VVC) is one o...
Combined application of antisense oligomers to control transcription factors of...
Araújo, Daniela Eira; Gonçalves, Bruna; Vilas-Boas, Diana Patrícia Andrade; Rodrigues, M. Elisa; Henriques, Mariana; Silva, Sónia CarinaAntisense oligomers (ASOs) have been little exploited to control determinants of Candida albicans virulence. Biofilm formation is an important virulence factor of C. albicans, that is regulated by a complex network of transcription factors (such as EFG1, BRG1 and ROB1). Thus, the main goal of this work was to project ASOs, based on the 2'-OMethyl chemical modification, to target BRG1 and ROB1 mRNA and to valida...
Simulated Vaginal Fluid: Candida resistant strains biofilm characterization and...
Fernandes, Liliana Sousa; Costa, Raquel; Henriques, Mariana; Rodrigues, M. ElisaIntroduction : Vulvovaginal candidiasis is a disease that affects millions of women worldwide. Oral formulations, topical creams or ointments are the conventional dosage forms, with an increase in drug administration through vaginal via. The use of simulated biological fluids (e.g. vaginal fluid) in the evaluation of antifungal therapies may better mimic the real biological environments and therefore provide a ...
Controlling ETEC colonization on cultures of an intestinal pig cell line with a...
Ferreira, Alice Maria Fernandes; Almeida, Carina; Silva, Daniela; Rodrigues, M. Elisa; Henriques, Mariana; Azeredo, Joana; Silva, Sónia CarinaEnterotoxigenic Escherichia coli (ETEC) colonizes the intestine of young pigs causing severe diarrhoea and consequently bringing high producing costs. The rise of antibiotic selective pressure together with on-going limitation on their use demands news strategies to tackle this pathology. The pertinence of using phages to tackle this problematic is being explored, and in this work, the efficacy of a T4-like pha...
Antisense locked nucleic acid gapmers to control Candida albicans filamentation
Araújo, Daniela; Mil-Homens, Dalila; Rodrigues, M. Elisa; Henriques, Mariana; Jørgensen, Per Trolle; Wengel, Jesper; Silva, Sónia CarinaWhereas locked nucleic acid (LNA) has been extensively used to control gene expression, it has never been exploited to control Candida virulence genes. Thus, the main goal of this work was to compare the efficacy of five different LNA-based antisense oligonucleotides (ASO) with respect to the ability to control EFG1 gene expression, to modulate filamentation and to reduce C. albicans virulence. In vitro, all LN...
Comparing the effect of Thymus spp. essential oils on Candida auris
Ribeiro, Rita; Fernandes, Liliana Sousa; Costa, Raquel; Cavaleiro, Carlos; Salgueiro, Lígia; Henriques, Mariana; Rodrigues, M. ElisaCandida auris is an emergent yeast pathogen responsible for many invasive fungal infections due to its multidrug-resistant character and its huge transmission ability. Essential oils (EOs) obtained from several aromatic plants have been regarded as an alternative treatment upon to fungal infections. For example, Thymus spp. are known by their antifungal effect due to the presence of some volatile compounds in t...
Candida albicans antimicrobial and antibiofilm activity of novel endodontic sol...
Ferreira, Inês; Rodrigues, M. Elisa; Fernandes, Liliana Sousa; Henriques, Mariana; Pina-Vaz, IreneBackground: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures ...
Candida spp./Bacteria Mixed Biofilms
Rodrigues, M. Elisa; Gomes, Fernanda Isabel; Rodrigues, Célia FortunaThe ability to form biofilms is a common feature of microorganisms, such as bacteria or fungi. These consortiums can colonize a variety of surfaces, such as host tissues, dentures, and catheters, resulting in infections highly resistant to drugs, when compared with their planktonic counterparts. This refractory effect is particularly critical in polymicrobial biofilms involving both fungi and bacteria. This rev...
Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity...
Gomes, Fernanda Isabel; Dias, Maria Inês; Lima, Â.; Barros, Lillian; Rodrigues, M. Elisa; Ferreira, Isabel C. F. R.; Henriques, MarianaMedicinal and aromatic plants are known to have a wide range of uses and health benefits, and should be exploited for their bioactivity. Here we evaluated the antimicrobial activity of decoctions of Satureja montana L. and Origanum majorana L. against Gram-positive and Gram-negative bacteria and Candida spp. as well as their mechanism of action and phenolic characterization. The Satureja montana and Origanum ma...
Application of 2OMethylRNA antisense oligomer to control Candida albicans EFG1 ...
Araújo, Daniela; Azevedo, N.; Barbosa, Ana; Almeida, Carina; Rodrigues, M. Elisa; Henriques, Mariana; Silva, Sónia CarinaAntisense oligomers and their analogues have been successfully utilized to silence gene expression for the treatment of many human diseases; however the control of yeast´s virulence determinants has never been exploited before. In this sense, this work is based on the key hypothesis that if a pathogens genetic sequence is a determinant of virulence, it will be possible to synthesize a nucleic acid mimic based o...