6 documents found, page 1 of 1
Immunomodulatory Properties of Umbilical Cord Blood-Derived Small Extracellular...
Rodrigues, Sílvia C.; Cardoso, Renato M. S.; Freire, Patricia C.; Gomes, Cláudia F.; Duarte, Filipe V.; Neves, Ricardo Pires das; Simões-Correia, JoanaUmbilical cord blood (UCB) has long been seen as a rich source of naïve cells with strong regenerative potential, likely mediated by paracrine signals. More recently, small extracellular vesicles (sEV), such as exosomes, have been shown to play essential roles in cell-to-cell communication, via the transport of numerous molecules, including small RNAs. Often explored for their potential as biomarkers, sEV are n...
Usefulness of universal beta-blocker therapy in patients after ST-elevation myo...
Ferreira, João André; Baptista, Rui Miguel; Rodrigues, Sílvia C.; Gonçalves, Lino ManuelThe use of beta-blockers (BB) in the context of ST-segment elevation myocardial infarction (STEMI) was a universal practice in the pre-reperfusion era. Since then, evidence of their use for secondary prevention after STEMI is scarce. Our aim is to determine treatment results associated with BB therapy after a STEMI at 1-year follow-up in a contemporary nationwide cohort.A prospective analysis involving 49 natio...
Toxicological Profile of Umbilical Cord Blood-Derived Small Extracellular Vesicles
Rodrigues, Sílvia C.; Cardoso, Renato M. S.; Gomes, Claudia F.; Duarte, Filipe Valente; Freire, Patricia C.; Neves, Ricardo; Correia, Joana SimõesThe development and adoption of cell therapies has been largely limited by difficulties associated with their safety, handling, and storage. Extracellular vesicles (EV) have recently emerged as a likely mediator for the therapeutic effect of cells, offering several advantages over cell therapies. Due to their small size and inability to expand and metastasize, EV are generally considered safer than cell transpl...
Development of an optimized and scalable method for isolation of umbilical cord...
Cardoso, Renato M. S.; Rodrigues, Sílvia C.; Gomes, Claudia F.; Duarte, Filipe V.; Romao, Maryse; Leal, Ermelindo C.; Freire, Patricia C.Extracellular vesicles (EV) are a promising therapeutic tool in regenerative medicine. These particles were shown to accelerate wound healing, through delivery of regenerative mediators, such as microRNAs. Herein we describe an optimized and upscalable process for the isolation of EV smaller than 200 nm (sEV), secreted by umbilical cord blood mononuclear cells (UCB-MNC) under ischemic conditions and propose qua...
Mitochondrial microRNAs: A Putative Role in Tissue Regeneration
Rodrigues, Sílvia C.; Cardoso, Renato M. S.; Duarte, Filipe V.The most famous role of mitochondria is to generate ATP through oxidative phosphorylation, a metabolic pathway that involves a chain of four protein complexes (the electron transport chain, ETC) that generates a proton-motive force that in turn drives the ATP synthesis by the Complex V (ATP synthase). An impressive number of more than 1000 mitochondrial proteins have been discovered. Since mitochondrial protein...
Neonatal Apex Resection Triggers Cardiomyocyte Proliferation, Neovascularizatio...
Sampaio-Pinto, Vasco; Rodrigues, Sílvia C.; Laundos, Tiago L.; Silva, Elsa D.; Vasques-Nóvoa, Francisco; Silva, Ana C.; Cerqueira, Rui J.So far, opposing outcomes have been reported following neonatal apex resection in mice, questioning the validity of this injury model to investigate regenerative mechanisms. We performed a systematic evaluation, up to 180 days after surgery, of the pathophysiological events activated upon apex resection. In response to cardiac injury, we observed increased cardiomyocyte proliferation in remote and apex regions,...