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Variability in the recognition of distinctive immunofluorescence patterns in di...

Dellavance,Alessandra; Cruvinel,Wilson de Melo; Francescantonio,Paulo Luiz Carvalho; Mangueira,Cristovão Luis Pitangueira; Drugowick,Inês Cristina

INTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the...

Date: 2013   |   Origin: Oasisbr
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