27 documents found, page 1 of 3
Practical Guidance on the Detection of NTRK Fusions in Sarcomas: Current Status...
Fernandes, Isabel; Macedo, Daniela; Gouveia, Emanuel; Ferreira, Ana; Lima, Jorge; Lopez, Dolores; Melo-Alvim, Cecília; Carvalho, Alice; Tavares, PauloSarcomas are a rare and heterogeneous group of mesenchymal malignant tumors and account for approximately 1% of all adult cancers and around 20% of all pediatric solid tumors in Europe. Technology advances have enabled a more accurate and efficient characterization of the molecular mechanisms underlying the pathogenesis of sarcoma subtypes and revealed novel and unexpected therapeutic targets with prognostic/pr...
Persistent Proinflammatory Cytokine Profile in the Tear Fluid of Stable Keratoc...
Gil, Pedro; Quadrado Gil, João; Cruz, Nuno; Costa, Celso; Rodrigues-Santos, Paulo; Sousa, Luana Madalena; Almeida, Jani Sofia; Fernandes, RosaPurpose: Keratoconus is traditionally classified as a noninflammatory corneal ectasia, despite growing evidence suggesting an underlying inflammatory component. This study evaluates whether patients with stable keratoconus exhibit persistent inflammatory activity in tear fluid compared to healthy controls. Methods: Cross-sectional case-control study. Keratoconus progression was evaluated using tomographic and c...
Comprehensive Evaluation of Peripheral Immunodynamics in Advanced Non-Small Cel...
Rodrigues-Santos, Paulo; Sousa, Luana Madalena; Almeida, Jani Sofia; Barata, Fernando; Figueiredo, AnaIntroduction: Immunotherapy, particularly immune checkpoint inhibitors (ICIs), has transformed the treatment landscape for advanced non-small cell lung cancer (NSCLC). Despite significant benefits for some patients, durable responses are limited to 20%–30% of cases, necessitating predictive biomarkers for better therapeutic guidance. While tumor-based markers such as PD-L1 expression and tumor mutational burden...
Connexin43 promotes exocytosis of damaged lysosomes through actin remodelling
Domingues, Neuza S.; Catarino, Steve; Cristóvão, Beatriz; Rodrigues, Lisa; Carvalho, Filomena A; Sarmento, Maria João; Zuzarte, MónicaA robust and efficient cellular response to lysosomal membrane damage prevents leakage from the lysosome lumen into the cytoplasm. This response is understood to happen through either lysosomal membrane repair or lysophagy. Here we report exocytosis as a third response mechanism to lysosomal damage, which is further potentiated when membrane repair or lysosomal degradation mechanisms are impaired. We show that ...
Natural Killer T-like Cells: Immunobiology and Role in Disease
Almeida, Jani-Sofia; Casanova, José M.; Santos-Rosa, Manuel; Tarazona, Raquel; Solana, Rafael; Rodrigues-Santos, PauloCD56+ T cells are generally recognized as a distinct population of T cells and are categorized as NKT-like cells. Although our understanding of NKT-like cells is far from satisfactory, it has been shown that aging and a number of disease situations have impacted these cells. To construct an overview of what is currently known, we reviewed the literature on human NKT-like cells. NKT-like cells are highly differe...
Dynamics of Soluble Factors and Double-Negative T Cells Associated with Respons...
Silva, Joana Delgado; Rodrigues-Santos, Paulo; Almeida, Jani Sofia; Santos Rosa, Manuel; Gonçalves, LinoThe role of the immune system, and hence inflammation, in the pathophysiology of hypertensive patients is not clear. Until now, most clinical and biochemical parameters have failed to predict a positive response to renal denervation (RDN). Our aim was to evaluate the immune response in a cohort of patients treated by RDN, through the analysis of cytokine, chemokine, and growth factor behavior. A population of 2...
Tumor-Infiltrating Lymphocytes and Cancer Markers in Osteosarcoma: Influence on...
Casanova, José Manuel; Almeida, Jani-Sofia; Reith, John David; Sousa, Luana Madalena; Fonseca, Rúben; Freitas-Tavares, Paulo; Santos-Rosa, ManuelOsteosarcoma (OST) is the most common type of high-grade primary bone tumor, which mainly affects young adults. The current standard of care for OST combines surgical resection with chemotherapy. The clinical outcomes and the current options to treat OST patients are unsatisfactory and novel treatment strategies are needed. The crosstalk between tumor cells and immune cells is essential to the OST microenvironm...
Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implic...
Mendes, João; Rodrigues-Santos, Paulo; Areia, Ana Luísa; Almeida, Jani-Sofia; Alves, Vera; Santos-Rosa, Manuel; Mota-Pinto, AnabelaBackground Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveri...
Acute MDPV Binge Paradigm on Mice Emotional Behavior and Glial Signature
Campeão, Mafalda; Fernandes, Luciana; Pita, Inês R.; Lemos, Cristina; Ali, Syed F.; Carvalho, Félix; Rodrigues-Santos, Paulo; Ribeiro, Carlos A. Fontes3,4-Methylenedioxypyrovalerone (MDPV), a widely available synthetic cathinone, is a popular substitute for classical controlled drugs of abuse, such as methamphetamine (METH). Although MDPV poses public health risks, its neuropharmacological profile remains poorly explored. This study aimed to provide evidence on that direction. Accordingly, C57BL/6J mice were exposed to a binge MDPV or METH regimen (four intra...
Tumor and Peripheral Immune Status in Soft Tissue Sarcoma: Implications for Imm...
Sousa, Luana Madalena; Almeida, Jani Sofia; Fortes-Andrade, Tânia; Santos Rosa, Manuel; Tavares, Paulo; Casanova, José Manuel; Rodrigues-Santos, PauloSoft Tissue Sarcomas (STS) are a heterogeneous and rare group of tumors. Immune cells, soluble factors, and immune checkpoints are key elements of the complex tumor microenvironment. Monitoring these elements could be used to predict the outcome of the disease, the response to therapy, and lead to the development of new immunotherapeutic approaches. Tumor-infiltrating B cells, Natural Killer (NK) cells, tumor-a...