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Reverse methanogenesis and respiration in methanotrophic archaea

Timmers, P. H. A.; Welte, C. U.; Koehorst, J. J.; Plugge, C. M.; Jetten, M. S. M.; Stams, Alfons Johannes Maria

Anaerobic oxidation of methane (AOM) is catalyzed by anaerobic methane-oxidizing archaea (ANME) via a reverse and modified methanogenesis pathway. Methanogens can also reverse the methanogenesis pathway to oxidize methane, but only during net methane production (i.e., "trace methane oxidation"). In turn, ANME can produce methane, but only during net methane oxidation (i.e., enzymatic back flux). Net AOM is exer...


Growth of anaerobic methane-oxidizing archaea and sulfate reducing bacteria in ...

Timmers, Peer H. A.; Gieteling, Jarno; Widjaja-Greefkes, H. C. Aura; Plugge, C. M.; Stams, Alfons Johannes Maria; Lens, Piet N. L.; Meulepas, Roel J. W.

Communities of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB) grow slowly, which limits the ability to perform physiological studies. High methane partial pressure was previously successfully applied to stimulate growth, but it is not clear how different ANME subtypes and associated SRB are affected by it. Here, we report on the growth of ANME-SRB in a membrane capsule bioreactor...


Growth and activity of ANME clades with different sulfate and sulfide concentra...

Timmers, Peer H. A.; Widjaja-Greefkes, H. C. Aura; Ramiro-Garcia, Javier; Plugge, C. M.; Stams, Alfons Johannes Maria

Extensive geochemical data showed that significant methane oxidation activity exists in marine sediments. The organisms responsible for this activity are anaerobic methane-oxidizing archaea (ANME) that occur in consortia with sulfate-reducing bacteria. A distinct zonation of different clades of ANME (ANME-1, ANME-2a/b and ANME-2c) exists in marine sediments, which could be related to the localized concentration...


Isolation and characterization of two novel thermophilic anaerobic bacteria fro...

Alves, J. I.; Alves, M. M.; Stams, Alfons Johannes Maria; Plugge, C. M.; Sousa, D. Z.

Syn(thesis)gas is a mixture containing hydrogen, carbon monoxide and carbon dioxide, in variable ratios. Syngas is commonly produced from fossil fuels, but it can be generated from a vast array of feedstocks such as lignocellulosic biomass and carbon-based wastes, including recalcitrant wastes. Production of biofuels and bulk chemicals from syngas, both by thermochemical or microbial processes, is a field of pr...


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