6 documents found, page 1 of 1
Metabolic composition of the cyanobacterium Nostoc muscorum as a function of cu...
Macário, I. P. E.; Veloso, T.; Romão, J.; Gonçalves, F. J. M.; Pereira, J. L; Duarte, I. F.; Ventura, S. P. M.Cyanobacteria are considered a factory of added-value compounds. However, knowledge about the array of interesting compounds that could be extracted from these prokaryotic organisms is still very limited. Nuclear Magnetic Resonance (NMR) spectroscopy is a widely used technique for metabolic profiling that allows an overview of the main metabolites present in complex biological matrices. In this work, high resol...
Inflammatory responses of a human keratinocyte cell line to 10 nm citrate- and ...
Bastos, V.; Brown, D.; Johnston, H.; Daniel-da-Silva, A. L.; Duarte, I. F.; Santos, C.; Oliveira, H.Silver nanoparticles (AgNPs) are among the most commonly used engineered NPs and various commercially available products are designed to come in direct contact with the skin (wound dressings, textiles, creams, among others). Currently, there is limited understanding of the influence of coatings on the toxicity of AgNPs and in particular their ability to impact on AgNP's mediated inflammatory responses. As AgNPs...
The influence of Citrate or PEG coating on silver nanoparticle toxicity to a hu...
Bastos, V.; Ferreira de Oliveira, J. M. P.; Brown, D.; Jonhston, H.; Malheiro, E.; Daniel-da-Silva, A. L.; Duarte, I. F.; Santos, C.; Oliveira, H.Surface coating of silver nanoparticles may influence their toxicity, in a way yet to decipher. In this study, human keratinocytes (HaCaT cells) were exposed for 24 and 48 h to well-characterized 30 nm AgNPs coated either with citrate (Cit30 AgNPs) or with poly(ethylene glycol) (PEG30 AgNPs), and assessed for cell viability, reactive oxygen species (ROS), cytokine release, apoptosis and cell cycle dynamics. The...
Different responses of young and expanded lettuce leaves to fungicide Mancozeb:...
Dias, M. C.; Figueiredo, P.; Duarte, I. F.; Gil, A. M.; Santos, C.This work aimed to study the effects of commercial doses of the fungicide, Mancozeb, on the photosynthetic apparatus of lettuce young leaves (YL) and expanded leaves (EL). Seven days after Mancozeb application, chlorophyll a fluorescence, pigment contents, lipid peroxidation, and proline content were evaluated. Independently of leaf age, Mancozeb treatment reduced the efficiency of photosystem II photochemistry...
Different responses of young and expanded lettuce leaves to fungicide Mancozeb:...
Dias, M. C.; Figueiredo, P.; Duarte, I. F.; Gil, A. M.; Santos, C.This work aimed to study the effects of commercial doses of the fungicide, Mancozeb, on the photosynthetic apparatus of lettuce young leaves (YL) and expanded leaves (EL). Seven days after Mancozeb application, chlorophyll a fluorescence, pigment contents, lipid peroxidation, and proline content were evaluated. Independently of leaf age, Mancozeb treatment reduced the efficiency of photosystem II photochemistry...
Potential Markers of Cisplatin Treatment Response Unveiled by NMR Metabolomics ...
Duarte, I. F.; Ladeirinha, A. F.; Lamego, I.; Gil, A. M.; Carvalho, L.; Carreira, I. M.; Melo, J. B.In this work, H-1 high resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was used to characterize the variations in the metabolome (small metabolites and mobile lipids) of A549 human lung cells in response to exposure to the alkylating drug cisplatin. Multivariate analysis and signal integration of spectral data were carried out to unveil exposure-induced effects and follow t...