184 documents found, page 1 of 19

Conselho Científico do INSA: em prol da investigação científica e desenvolvimen...

Uma das principais atribuições do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) é promover e desenvolver atividades de investigação científica e desenvolvimento tecnológico (IC&DT) contribuindo para ganhos em saúde pública. O Conselho Científico (CC) do INSA é o órgão responsável pela apreciação e acompanhamento das atividades de IC&DT, órgão de reflexão e debate das atividades científicas do INSA, e ...

Genómica funcional – a investigação do RNA mensageiro e da proteína dele result...

A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated ...

Eukaryotic cells possess surveillance mechanisms that detect and degrade defective transcripts. Aberrant transcripts include mRNAs with a premature termination codon (PTC), targeted by the nonsense-mediated decay (NMD) pathway, and mRNAs lacking a termination codon, targeted by the nonstop decay (NSD) pathway. The eukaryotic exosome, a ribonucleolytic complex, plays a crucial role in mRNA processing and turnove...

The role of UPF1 cap-independent translation in colorectal cancer

Colorectal cancer (CRC) is one of the deadliest diseases worldwide with projections pointing towards an increase for the next two decades. Translation dysregulation of many genes contributes to CRC development, and here we are studying the role of translation dysregulation of up-frameshift 1 (UPF1) in CRC. This protein is involved in many cellular mechanisms such as nonsense-mediated mRNA decay, cell cycle prog...

A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated ...

Eukaryotic cells possess surveillance mechanisms that detect and degrade defective transcripts. Aberrant transcripts include mRNAs with a premature termination codon (PTC), targeted by the nonsense-mediated decay (NMD) pathway, and mRNAs lacking a termination codon, targeted by the nonstop decay (NSD) pathway. The eukaryotic exosome, a ribonucleolytic complex, plays a crucial role in mRNA processing and turnove...

DIS3L2 knockdown impairs key oncogenic properties of colorectal cancer cells vi...

DIS3L2 degrades different types of RNAs in an exosome-independent manner including mRNAs and several types of non-coding RNAs. DIS3L2-mediated degradation is preceded by the addition of nontemplated uridines at the 3’end of its targets by the terminal uridylyl transferases 4 and 7. Most of the literature that concerns DIS3L2 characterizes its involvement in several RNA degradation pathways, however, there is so...

Non-canonical synthesis of UPF1 protein contributes to its oncogenic role in co...

Colorectal cancer (CRC) is the third leading cause worldwide and projections point towards an increase over the next two decades. Gene expression dysregulation of several genes involved in CRC contribute to disease development. The up-frameshift 1 (UPF1) protein plays important roles in several cellular mechanisms and acts as a tumour suppressor in most cancers. However, in CRC, this protein has been described ...

Internal ribosome entry site-mediated translation of UPF1 contributes to its on...

Colorectal cancer (CRC) is the third cause of death worldwide and projections point towards an increase for the next two decades. Many genes are misregulated in CRC, contributing to the development of the disease. Up-frameshift 1 (UPF1) is involved in many cellular mechanisms such as nonsense-mediated mRNA decay, cell cycle progression, or telomere maintenance and homeostasis. It also works as a tumour suppress...

The p53 short isoforms are activated by the integrated stress response to enhan...

Introduction: The full-length p53 (FLp53) protein is a transcriptional factor that mediates cellular stress responses. The appropriate response is modulated according to the nature and extent of the damage, which means that p53 may promote cell cycle arrest and DNA repair or apoptosis in different situations. In all cases, p53 manages the response by modulating the expression of a wide range of target genes, as...

Translational Regulation of the Human PERK by Upstream Open Reading Frames

Upstream open reading frames (uORFs) are cis-acting elements located within the 5’ leader sequence (5’UTR) of transcripts, which can regulate translation of the correspondent main open reading frame (mORF). During endoplasmic reticulum (ER) stress, the accumulation of unfolded proteins activates the ER-resident PKR-like ER kinase (PERK), which results in phosphorylation of eIF2α to inhibit global mRNA translati...